구절초 추출물의 nitric oxide와 inducible nitric oxide synthase의 발현 억제 효과 Suppressive Effects of Chrysanthemum zawadskii var. latilobum on Nitric Oxide Production and Inducible Nitric Oxide Synthase Expression원문보기
The activation of iNOS induces the formation of large amount of nitric oxide (NO), which plays a key role in the pathogenesis of a variety of inflammatory diseases. Therefore, agents that inhibit iNOS expression and/or enzyme activity resulting in decreased NO generation may have therapeutic benefic...
The activation of iNOS induces the formation of large amount of nitric oxide (NO), which plays a key role in the pathogenesis of a variety of inflammatory diseases. Therefore, agents that inhibit iNOS expression and/or enzyme activity resulting in decreased NO generation may have therapeutic beneficial effect in the treatment of inflammatory diseases. Activation of NF-κB and mitogen-activated protein kinase (MAPK) is critical in the induction of iNOS in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Chrysanthemum zawadskii var. latilobum (Compositae), commonly known as Gu-Jul-Cho in Korea, is used in traditional medicine to treat pneumonia, bronchitis, cough, common cold, pharyngitis, bladder-related disorders, gastroenteric disorders, and hypertension. Although the anti-cancer, antioxidant, and anti-microbial effects of C. zawadskii extract have been reported, anti-inflammatory effect has not yet to be studied. Therefore, we investigated the effect of C. zawadskii extract on NO production, iNOS expression, and NF-κB activity in LPS-induced RAW 264.7 macrophage cells. The results show that C. zawadskii extract (5-50 μg/mL) significantly inhibited LPS-induced NO production in a concentration-dependent manner. Consistent with the inhibitory effect on NO production, C. zawadskii extract inhibited the expression of iNOS and mRNA expression. Furthermore, C. zawadskii extract suppressed NF-κB (p50) translocation to the nucleus as well as I-κB release resulting in the inhibition of the DNA binding activity of the NF-κB. C. zawadskii extract also inhibited extracellular signal-regulated kinase (ERK) signal, which is the most significant signal in NO production and NF-κB activation. Flower and leaf extracts of C. zawadskii showed a strong anti-inflammatory activity. At the same concentrations, the flower extract of C. zawadskii have stronger anti-inflammatory activity than leaf extract of C. zawadskii. The present results suggest that C. zawadskii extract has an inhibitory effect on NO production through the inhibition of NF-κB/MAPK activity, and thus can be used as an anti-inflammatory agent.
The activation of iNOS induces the formation of large amount of nitric oxide (NO), which plays a key role in the pathogenesis of a variety of inflammatory diseases. Therefore, agents that inhibit iNOS expression and/or enzyme activity resulting in decreased NO generation may have therapeutic beneficial effect in the treatment of inflammatory diseases. Activation of NF-κB and mitogen-activated protein kinase (MAPK) is critical in the induction of iNOS in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Chrysanthemum zawadskii var. latilobum (Compositae), commonly known as Gu-Jul-Cho in Korea, is used in traditional medicine to treat pneumonia, bronchitis, cough, common cold, pharyngitis, bladder-related disorders, gastroenteric disorders, and hypertension. Although the anti-cancer, antioxidant, and anti-microbial effects of C. zawadskii extract have been reported, anti-inflammatory effect has not yet to be studied. Therefore, we investigated the effect of C. zawadskii extract on NO production, iNOS expression, and NF-κB activity in LPS-induced RAW 264.7 macrophage cells. The results show that C. zawadskii extract (5-50 μg/mL) significantly inhibited LPS-induced NO production in a concentration-dependent manner. Consistent with the inhibitory effect on NO production, C. zawadskii extract inhibited the expression of iNOS and mRNA expression. Furthermore, C. zawadskii extract suppressed NF-κB (p50) translocation to the nucleus as well as I-κB release resulting in the inhibition of the DNA binding activity of the NF-κB. C. zawadskii extract also inhibited extracellular signal-regulated kinase (ERK) signal, which is the most significant signal in NO production and NF-κB activation. Flower and leaf extracts of C. zawadskii showed a strong anti-inflammatory activity. At the same concentrations, the flower extract of C. zawadskii have stronger anti-inflammatory activity than leaf extract of C. zawadskii. The present results suggest that C. zawadskii extract has an inhibitory effect on NO production through the inhibition of NF-κB/MAPK activity, and thus can be used as an anti-inflammatory agent.
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