초록 ▼

용어보기논문에서 용어와 풀이말을 자동 추출한 결과로, 시범 서비스 중입니다. AI-Helper

건조된 붉나무 잎을 7일 동안 실온에서 메탄올로 추출한다. 획득된 유기물을 갑압 증류한 후 물에 녹인 다음에 n-hexane, ethyl ether, ethyl acetate and BuOH 로 분리한다. ethyl acetate 부분을 반복하여 ...

건조된 붉나무 잎을 7일 동안 실온에서 메탄올로 추출한다. 획득된 유기물을 갑압 증류한 후 물에 녹인 다음에 n-hexane, ethyl ether, ethyl acetate and BuOH 로 분리한다. ethyl acetate 부분을 반복하여 column chromatography 에서 분리 하여 quercitrin 과 afzelin 을 얻었다. 분리된 quercitrin 을 이용하여 본 연구자는 ester 유도체를 합성 하였다. 두 천연물과 합성 화합물 구조는 Mass spectroscopy 와 광범위하게 사용하는 NMR (¹H, ^(13)C and 2D-NMR) 을 사용하여 확인하였다. 대식세포 (RAW 264.7 cells)를 lipopolysaccharide 로 자극 하여 nitric oxide (NO)의 생성과 iNOS 등을 억제 시키는 두 천연물과 합성 화합물에서 본 연구자는 검토 하고자 한다.

Abstract ▼ AI-Helper

Dried and grinded leaves of Rhus chinensis was extracted with methanol at room temperature for 7 days. The obtained organic phase was evaporated at reduced pressure. It was then dissolved in water and partitioned successvely with n-hexane, ethyl ether, ethyl acetate and BuOH. The ethyl acetate fract...

Dried and grinded leaves of Rhus chinensis was extracted with methanol at room temperature for 7 days. The obtained organic phase was evaporated at reduced pressure. It was then dissolved in water and partitioned successvely with n-hexane, ethyl ether, ethyl acetate and BuOH. The ethyl acetate fraction was fractionated by using repeated column chromatography and it gave rise quercitrin and afzelin. Using isolated quercitrin we synthesized ester derivatives. Structure of these two natural compounds and two synthesis compounds was confirmed through Mass spectroscopy and extensive NMR (¹H, ^(13)C and 2D NMR) studies. In murine cells (RAW 264.7 cells), processed by lipopolysaccharide, these two natural compounds and two synthesis compounds inhibited the creation of NO and PGE2 that would cause over expression of COX-2 and i NOS.