근권토양으로부터 항균활성이 강한 세균을 분리하였다. 이 세균은 16S rRNA gene서열 분석 결과 Lysobacter antibioticus로 동정되었고 L. antibioticus HS124로 명명하였다. HS124는 chitinase, β-1,3-glucanase, lipase, protease, siderophores와 같은 다양한 ...
근권토양으로부터 항균활성이 강한 세균을 분리하였다. 이 세균은 16S rRNA gene서열 분석 결과 Lysobacter antibioticus로 동정되었고 L. antibioticus HS124로 명명하였다. HS124는 chitinase, β-1,3-glucanase, lipase, protease, siderophores와 같은 다양한 이차대사산물을 분비할 뿐만 아니라, HPLC와 GC-MS분석을 통해 강력한 항생물질, 4-hydroxyphenylacetic acid를 생성함을 확인하였다. 또한 철 (FeCl₃·6H₂O, 100 μM)을 첨가한 배지에서 HS124를 배양한 결과 대조구에 비해 항균활성이 약 두 배 증가 하였다. 포트 재배에서 고추역병에 대한 HS124의 방제효과를 조사하기 위해 고추 정식 후 2, 3, 4, 5주째에 철을 첨가한 배양액(C1)과 첨가하지 않은 배양액(C2), 대조구로서 철을 넣은 배지(M1)와 넣지 않은 배지(M2)를 각각 30 ml씩 처리하였으며, 6주째에 P. capsici의 유주자(1×10^(6) zoospore ml^(-1)) 30 ml를 접종하였다. 병원균 접종 후 0, 2, 4, 6, 8일 실험기간 동안 생체중, 뿌리치사율, 병발생율, pathogenesis-related proteins (PRs)을 조사하였다. C1과 C2처리구는 M1과 M2에 비해 매우 높은 생체중과, 낮은 뿌리치사율을 보였으며, 특히 C1는 M2처리구에 비해 약 70%의 병방제율을 보였다. 또한 PRs를 조사한 결과, 처리구 모두 뿌리에서는 감소하였고 잎에서는 증가하는 경향을 보였으나, M1과 M2에서 그 변화폭이 훨씬 컸다. 따라서 강한 항균 활성능력을 지닌 L. antibioticus HS124는 고추역병에 생물학적 방제제로서 가치가 있다고 사료된다.
근권토양으로부터 항균활성이 강한 세균을 분리하였다. 이 세균은 16S rRNA gene서열 분석 결과 Lysobacter antibioticus로 동정되었고 L. antibioticus HS124로 명명하였다. HS124는 chitinase, β-1,3-glucanase, lipase, protease, siderophores와 같은 다양한 이차대사산물을 분비할 뿐만 아니라, HPLC와 GC-MS분석을 통해 강력한 항생물질, 4-hydroxyphenylacetic acid를 생성함을 확인하였다. 또한 철 (FeCl₃·6H₂O, 100 μM)을 첨가한 배지에서 HS124를 배양한 결과 대조구에 비해 항균활성이 약 두 배 증가 하였다. 포트 재배에서 고추역병에 대한 HS124의 방제효과를 조사하기 위해 고추 정식 후 2, 3, 4, 5주째에 철을 첨가한 배양액(C1)과 첨가하지 않은 배양액(C2), 대조구로서 철을 넣은 배지(M1)와 넣지 않은 배지(M2)를 각각 30 ml씩 처리하였으며, 6주째에 P. capsici의 유주자(1×10^(6) zoospore ml^(-1)) 30 ml를 접종하였다. 병원균 접종 후 0, 2, 4, 6, 8일 실험기간 동안 생체중, 뿌리치사율, 병발생율, pathogenesis-related proteins (PRs)을 조사하였다. C1과 C2처리구는 M1과 M2에 비해 매우 높은 생체중과, 낮은 뿌리치사율을 보였으며, 특히 C1는 M2처리구에 비해 약 70%의 병방제율을 보였다. 또한 PRs를 조사한 결과, 처리구 모두 뿌리에서는 감소하였고 잎에서는 증가하는 경향을 보였으나, M1과 M2에서 그 변화폭이 훨씬 컸다. 따라서 강한 항균 활성능력을 지닌 L. antibioticus HS124는 고추역병에 생물학적 방제제로서 가치가 있다고 사료된다.
An antagonistic bacterium having a broad spectrum activity against several fungal pathogens was isolated from rhizosphere soil and identified as Lysobacter antibioticus (named as HS124) on the basis of 16S rRNA gene sequence. This strain HS124 produced a number of secondary metabolites such as chiti...
An antagonistic bacterium having a broad spectrum activity against several fungal pathogens was isolated from rhizosphere soil and identified as Lysobacter antibioticus (named as HS124) on the basis of 16S rRNA gene sequence. This strain HS124 produced a number of secondary metabolites such as chitinase, β-1,3-glucanase, lipase, protease and siderophores. Also, the bacterium released an antibiotic compound, 4-hydroxyphenylacetic acid which was identified using HPLC, GC-MS. When HS124 was grown on broth supplemented with Fe(III) 100 ?M, their antifungal activity against Phytophthora capsici was approximately two times higher than broth without Fe(III). To investigate the protective effect of HS124 against phytophthora blight in pepper caused by P. capsici, four weeks old seedlings were transplanted into the pots. At 2, 3, 4 and 5 weeks after transplanting, pepper plants were treated with 30 ml of each culture with (C1) and without (C2) or only medium with (M1) and without Fe(III) 100 ?M (M2), and then 30 ml of the zoospore suspension (1×10^(6) zoospore ml^(-1)) of P. capsici were infected to each treatment at 6 week after transplanting. During the experiment periods, growth of peppers treated with bacterial cultures (C1 and C2) was gradually enhanced, finally resulting in great protection from a disease compared to controls (M1 and M2); however, great growth and protection was found in C1 supplemented with Fe(III) 100 ?M. On the other hand, the activities of pathogenesis-related proteins such as chitinase and β-1,3-glucanase decreased in roots, but increased in leaves with time where the change was much greater in M1 and M2 treatments than in C1 and C2 treatments. In conclusion, we suggest that L. antibioticus HS124 producing the secondary metabolites can be a good candidate in biocontrol of P. capsici.
An antagonistic bacterium having a broad spectrum activity against several fungal pathogens was isolated from rhizosphere soil and identified as Lysobacter antibioticus (named as HS124) on the basis of 16S rRNA gene sequence. This strain HS124 produced a number of secondary metabolites such as chitinase, β-1,3-glucanase, lipase, protease and siderophores. Also, the bacterium released an antibiotic compound, 4-hydroxyphenylacetic acid which was identified using HPLC, GC-MS. When HS124 was grown on broth supplemented with Fe(III) 100 ?M, their antifungal activity against Phytophthora capsici was approximately two times higher than broth without Fe(III). To investigate the protective effect of HS124 against phytophthora blight in pepper caused by P. capsici, four weeks old seedlings were transplanted into the pots. At 2, 3, 4 and 5 weeks after transplanting, pepper plants were treated with 30 ml of each culture with (C1) and without (C2) or only medium with (M1) and without Fe(III) 100 ?M (M2), and then 30 ml of the zoospore suspension (1×10^(6) zoospore ml^(-1)) of P. capsici were infected to each treatment at 6 week after transplanting. During the experiment periods, growth of peppers treated with bacterial cultures (C1 and C2) was gradually enhanced, finally resulting in great protection from a disease compared to controls (M1 and M2); however, great growth and protection was found in C1 supplemented with Fe(III) 100 ?M. On the other hand, the activities of pathogenesis-related proteins such as chitinase and β-1,3-glucanase decreased in roots, but increased in leaves with time where the change was much greater in M1 and M2 treatments than in C1 and C2 treatments. In conclusion, we suggest that L. antibioticus HS124 producing the secondary metabolites can be a good candidate in biocontrol of P. capsici.
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