Chitosan coated nano size liposomes have been used as carriers for hydrophilic and hydrophobic ingredients. The nanoliposomes have been prepared from phosphatidylcholine (pc), cholesterol (chol) and chitosan by sonication method. As hydrophilic carrier for vitamin C (VC), liposomes which were prepar...
Chitosan coated nano size liposomes have been used as carriers for hydrophilic and hydrophobic ingredients. The nanoliposomes have been prepared from phosphatidylcholine (pc), cholesterol (chol) and chitosan by sonication method. As hydrophilic carrier for vitamin C (VC), liposomes which were prepared by direct injection method using ethanol as solvent with 40:60 and 60:40 ratio of pc and chol has smaller mean diameter about 97.4nm and 95.8nm respectively. The highest loading efficiency and payload of VC in the liposomes were 96.5% and 46.82%. When liposomes were prepared with 100mg initial mass of VC, the highest loading efficiency could be obtained. Furthermore with the increasing of initial mass of VC, the payload was increased. VC loaded liposomes was stability during 15 weeks storage that can protect over 85% of VC against oxidation. As hydrophobic carrier of vitamin E (VE), at the 40:60 ratios of pc and cholesterol with 12 min sonication, the smallest liposomes, with the mean diameter about 82nm, were obtained. After VE was loaded to the liposomes, the size of chitosan-coated liposomes increased to 144nm. The loading efficiency and payload of VE were investigated by loading different amount of VE to the liposomes. Chitosan-coated nano-size liposomes appeared to be promising VE carrier with highest loading efficiency over 99% and payload over 27%. The stability of VE loaded liposomes suspension during the 8 weeks storage is over 90% under 4℃. A new multiplex carrier, chitosan coated nanoliposomes, have been used for both hydrophobic and hydrophilic ingredients together. The multi layers of nanoliposomes have been prove by Cryo-TEM. Chitosan coated nanoliposomes prepared by 0.1% chitosan solution,pc and chol with the ratio of 60 and 40, have sharp size distribution with the average size of 82nm and positive charge of zeta potential proven by quaselastic laser light scattering. With increasing the concentration of chitosan solution, the size of the nanoliposomes increased and got more positive charge to the surface of nanoliposomes. Incorporation of chemically labile active ingredients into chitosan coated nanoliposomes against chemical degradation, which are shown for VE and VC have as the model drug of hydrophobic and hydrophilic ingredients respectively. Because of the hydrophobic property, VE can be loaded to the lipid layers of chitosan coated nanoliposomes. VC is hydrophilic drug, so it can be encapsulated to the aqueous medium between the lipid layers of nanoliposomes. After the drugs have been loaded to the nanoliposomes, size of nanoliposomes increased to 134nm. The loading efficiencies of VE and VC are 97% and 56% respectively measured by HPLC. During storage the presents of VE improve the stability of VC in the nanoliposomes compared with the nanoliposomes which has only Vitamin C loaded. The coating material chitosan give the special character to the nanoliposomes to make it more stability and can protect the ingredients in the nanoliposomes during storage compare with non coated liposomes. Chitosan-coated nanoliposomes have been used as antitumor drug carrier by direct injection and film loading methods. Liposomes prepared by the direct injection method using ethanol as the solvent have smaller mean diameters (106 nm) than those prepared using the film loading method. After camptothecin (CPT)-loaded, the size of the liposomes prepared using direct injection with ethanol as the solvent increased to 193nm. The loading efficiency and payload of CPT were investigated by HPLC. Chitosan-coated nanoliposomes prepared by direct injection with ethanol as the solvent and a 60:40 ratio of pc:chol were promising CPT carriers since they have the highest loading efficiency (93.60%) and payload (37.20%). The liposomes have higher loading efficiency and payload when prepared using direct injection compared to those liposomes prepared using the film loading method. The in vitro test was performed using PBS pH 7.4. It was determined that CPT was slowly released and that 86% of the CPT was released in 72 hours.
Chitosan coated nano size liposomes have been used as carriers for hydrophilic and hydrophobic ingredients. The nanoliposomes have been prepared from phosphatidylcholine (pc), cholesterol (chol) and chitosan by sonication method. As hydrophilic carrier for vitamin C (VC), liposomes which were prepared by direct injection method using ethanol as solvent with 40:60 and 60:40 ratio of pc and chol has smaller mean diameter about 97.4nm and 95.8nm respectively. The highest loading efficiency and payload of VC in the liposomes were 96.5% and 46.82%. When liposomes were prepared with 100mg initial mass of VC, the highest loading efficiency could be obtained. Furthermore with the increasing of initial mass of VC, the payload was increased. VC loaded liposomes was stability during 15 weeks storage that can protect over 85% of VC against oxidation. As hydrophobic carrier of vitamin E (VE), at the 40:60 ratios of pc and cholesterol with 12 min sonication, the smallest liposomes, with the mean diameter about 82nm, were obtained. After VE was loaded to the liposomes, the size of chitosan-coated liposomes increased to 144nm. The loading efficiency and payload of VE were investigated by loading different amount of VE to the liposomes. Chitosan-coated nano-size liposomes appeared to be promising VE carrier with highest loading efficiency over 99% and payload over 27%. The stability of VE loaded liposomes suspension during the 8 weeks storage is over 90% under 4℃. A new multiplex carrier, chitosan coated nanoliposomes, have been used for both hydrophobic and hydrophilic ingredients together. The multi layers of nanoliposomes have been prove by Cryo-TEM. Chitosan coated nanoliposomes prepared by 0.1% chitosan solution,pc and chol with the ratio of 60 and 40, have sharp size distribution with the average size of 82nm and positive charge of zeta potential proven by quaselastic laser light scattering. With increasing the concentration of chitosan solution, the size of the nanoliposomes increased and got more positive charge to the surface of nanoliposomes. Incorporation of chemically labile active ingredients into chitosan coated nanoliposomes against chemical degradation, which are shown for VE and VC have as the model drug of hydrophobic and hydrophilic ingredients respectively. Because of the hydrophobic property, VE can be loaded to the lipid layers of chitosan coated nanoliposomes. VC is hydrophilic drug, so it can be encapsulated to the aqueous medium between the lipid layers of nanoliposomes. After the drugs have been loaded to the nanoliposomes, size of nanoliposomes increased to 134nm. The loading efficiencies of VE and VC are 97% and 56% respectively measured by HPLC. During storage the presents of VE improve the stability of VC in the nanoliposomes compared with the nanoliposomes which has only Vitamin C loaded. The coating material chitosan give the special character to the nanoliposomes to make it more stability and can protect the ingredients in the nanoliposomes during storage compare with non coated liposomes. Chitosan-coated nanoliposomes have been used as antitumor drug carrier by direct injection and film loading methods. Liposomes prepared by the direct injection method using ethanol as the solvent have smaller mean diameters (106 nm) than those prepared using the film loading method. After camptothecin (CPT)-loaded, the size of the liposomes prepared using direct injection with ethanol as the solvent increased to 193nm. The loading efficiency and payload of CPT were investigated by HPLC. Chitosan-coated nanoliposomes prepared by direct injection with ethanol as the solvent and a 60:40 ratio of pc:chol were promising CPT carriers since they have the highest loading efficiency (93.60%) and payload (37.20%). The liposomes have higher loading efficiency and payload when prepared using direct injection compared to those liposomes prepared using the film loading method. The in vitro test was performed using PBS pH 7.4. It was determined that CPT was slowly released and that 86% of the CPT was released in 72 hours.
주제어
#liposome
학위논문 정보
저자
劉枏
학위수여기관
Graduate School of Life Sciences and Biotechnology, Korea University
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