개똥쑥(Artemisia annua L.)은 잔잎쑥, 개땅쑥으로도 불리우며 길가나 빈터, 강가 등에서 자라고 높이가 약 1 m 정도에 이른다. 6～8월에 녹황색의 꽃이 피며 우리나라의 경기, 제주, 평북, 함남 지방이나 아시아 및 유럽 등지에 널리 분포되어 있다. 중국에서는 예로부터 말라리아 치료를 위한 약초로 사용되어 왔는데, sesquiterpene의 주성분인 artemisinin은 강력한 항말라리아 효능을 지니므로 현재 의약품으로 이용되고 있다. 개똥쑥의 정유성분으로는 ...
개똥쑥(Artemisia annua L.)은 잔잎쑥, 개땅쑥으로도 불리우며 길가나 빈터, 강가 등에서 자라고 높이가 약 1 m 정도에 이른다. 6～8월에 녹황색의 꽃이 피며 우리나라의 경기, 제주, 평북, 함남 지방이나 아시아 및 유럽 등지에 널리 분포되어 있다. 중국에서는 예로부터 말라리아 치료를 위한 약초로 사용되어 왔는데, sesquiterpene의 주성분인 artemisinin은 강력한 항말라리아 효능을 지니므로 현재 의약품으로 이용되고 있다. 개똥쑥의 정유성분으로는 linalool, 1,8-cineol, ρ-cymene, thujone, camphor 등이 존재하는 것으로 알려져 있으나, 국내에서 개똥쑥에 관한 연구는 거의 전무한 실정이다. 본 연구에서는 개똥쑥의 영양성분을 조사하였고, 생리활성에 관한 기초 연구로서 잎과 줄기로 구분한 후 각각 물 및 80% 에탄올로 추출하여 in vitro에서 항산화 활성 및 생리활성을 시험하였으며, 이와 관련된 성분인 페놀 화합물은 HPLC를 이용하여 분석하였다. 1. 개똥쑥 잎과 줄기의 일반성분을 정량한 결과 수분, 조지방 및 조단백질 함량은 잎에서 높았으나 회분 및 조섬유의 함량은 줄기에서 유의적으로 높았다. 무기물은 잎에서 9종, 줄기에서 6종이 검출되었으며, K 및 Ca의 함량이 가장 많았다. 구성아미노산은 잎에서 2356.10 mg%, 줄기는 996.34 mg%로 잎이 약 2배 이상 높았으며, glutamic acid의 함량이 가장 많았다. 유리아미노산 함량은 구성아미노산 함량과 유사한 경향으로 잎에서 약 1.4배 정도 높았으며, proline 함량이 가장 높아 총 아미노산의 50% 이상을 차지하였다. 2. 개똥쑥 잎과 줄기의 물 및 80%에탄올 추출물 중 총 페놀 함량은 잎의 물 및 에탄올 추출물에서 각각 88.19 mg/g과 99.98 mg/g였으며, 줄기에서는 각각 45.03 mg/g, 41.44 mg/g으로 줄기에 비해 잎에서 약 2배 정도 높은 함량이었다. 플라보노이드 함량은 총 페놀 함량과 유사한 경향으로 잎의 에탄올 추출물에서 51.86 mg/g으로 가장 높았다. 3. 개똥쑥 잎과 줄기의 추출물의 항산화 활성 및 생리활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl)․ABTS [2,2'-azinobis-(3-ethylbenzo-thiazoline-6 -sulfonate)]․NO (nitric oxide)라디칼 소거능, 환원력, FRAP (ferric-reducing antioxidantpotential ability)assay, Fe2+ chelating 활성, tyrosinase 및 α-glucosidase 저해활성을 시험하였다. 농도 의존적으로 그 활성이 증가하였으며, 이들의 활성은 대부분 줄기보다 잎에서 더 높은 경향을 보였다. 특히, DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 잎이 줄기에 비해 유의적으로 높았으나, 의외로 α-glucosidase 저해활성은 줄기에서 더 높았다. 4. 개똥쑥 추출물의 인체 유방암 세포인 MCF-7, 자궁경부 상피암 세포인 HeLa 및 위암세포인 AGS에 대한 증식억제 활성을 측정하였다. 시료의 첨가농도가 증가함에 따라 암세포 증식억제 활성이 유의적으로 상승되었으며 줄기보다 잎 추출물에서, 물 보다 에탄올 추출물에서 유의적으로 그 활성이 높았다. 특히, 잎 에탄올 추출물의 MCF-7, HeLa 및 AGS 세포에 대한 증식억제 활성은 250 μg/mL농도에서 각각 76.26%, 54.59% 및 43.52%로 추출물 중 가장 높은 활성을 보였다. 5. 개똥쑥 추출물로부터 HPLC를 이용하여 phenolic acid, flavonol 및 catechin을 분석한 결과, phenolic acid는 잎의 물 및 에탄올 추출물에서 각각 10종 및 9종, 줄기 추출물에서 각각 8종 및 6종이 동정되었다. Flavonol류는 잎에서만 동정되었으며, rutin 과 kaempferol은 잎 에탄올 추출물에서만 동정되었다. Catechin류는 줄기에 비해 잎에서 거의 모든 화합물이 훨씬 높게 정량되었으며 특히, 잎 에탄올 추출물은 catechin, gallocatechin gallate, epicatechin gallate 및 catechin gallate의 함량이 월등히 높았다.
개똥쑥(Artemisia annua L.)은 잔잎쑥, 개땅쑥으로도 불리우며 길가나 빈터, 강가 등에서 자라고 높이가 약 1 m 정도에 이른다. 6～8월에 녹황색의 꽃이 피며 우리나라의 경기, 제주, 평북, 함남 지방이나 아시아 및 유럽 등지에 널리 분포되어 있다. 중국에서는 예로부터 말라리아 치료를 위한 약초로 사용되어 왔는데, sesquiterpene의 주성분인 artemisinin은 강력한 항말라리아 효능을 지니므로 현재 의약품으로 이용되고 있다. 개똥쑥의 정유성분으로는 linalool, 1,8-cineol, ρ-cymene, thujone, camphor 등이 존재하는 것으로 알려져 있으나, 국내에서 개똥쑥에 관한 연구는 거의 전무한 실정이다. 본 연구에서는 개똥쑥의 영양성분을 조사하였고, 생리활성에 관한 기초 연구로서 잎과 줄기로 구분한 후 각각 물 및 80% 에탄올로 추출하여 in vitro에서 항산화 활성 및 생리활성을 시험하였으며, 이와 관련된 성분인 페놀 화합물은 HPLC를 이용하여 분석하였다. 1. 개똥쑥 잎과 줄기의 일반성분을 정량한 결과 수분, 조지방 및 조단백질 함량은 잎에서 높았으나 회분 및 조섬유의 함량은 줄기에서 유의적으로 높았다. 무기물은 잎에서 9종, 줄기에서 6종이 검출되었으며, K 및 Ca의 함량이 가장 많았다. 구성아미노산은 잎에서 2356.10 mg%, 줄기는 996.34 mg%로 잎이 약 2배 이상 높았으며, glutamic acid의 함량이 가장 많았다. 유리아미노산 함량은 구성아미노산 함량과 유사한 경향으로 잎에서 약 1.4배 정도 높았으며, proline 함량이 가장 높아 총 아미노산의 50% 이상을 차지하였다. 2. 개똥쑥 잎과 줄기의 물 및 80%에탄올 추출물 중 총 페놀 함량은 잎의 물 및 에탄올 추출물에서 각각 88.19 mg/g과 99.98 mg/g였으며, 줄기에서는 각각 45.03 mg/g, 41.44 mg/g으로 줄기에 비해 잎에서 약 2배 정도 높은 함량이었다. 플라보노이드 함량은 총 페놀 함량과 유사한 경향으로 잎의 에탄올 추출물에서 51.86 mg/g으로 가장 높았다. 3. 개똥쑥 잎과 줄기의 추출물의 항산화 활성 및 생리활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl)․ABTS [2,2'-azinobis-(3-ethylbenzo-thiazoline-6 -sulfonate)]․NO (nitric oxide)라디칼 소거능, 환원력, FRAP (ferric-reducing antioxidant potential ability)assay, Fe2+ chelating 활성, tyrosinase 및 α-glucosidase 저해활성을 시험하였다. 농도 의존적으로 그 활성이 증가하였으며, 이들의 활성은 대부분 줄기보다 잎에서 더 높은 경향을 보였다. 특히, DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 잎이 줄기에 비해 유의적으로 높았으나, 의외로 α-glucosidase 저해활성은 줄기에서 더 높았다. 4. 개똥쑥 추출물의 인체 유방암 세포인 MCF-7, 자궁경부 상피암 세포인 HeLa 및 위암세포인 AGS에 대한 증식억제 활성을 측정하였다. 시료의 첨가농도가 증가함에 따라 암세포 증식억제 활성이 유의적으로 상승되었으며 줄기보다 잎 추출물에서, 물 보다 에탄올 추출물에서 유의적으로 그 활성이 높았다. 특히, 잎 에탄올 추출물의 MCF-7, HeLa 및 AGS 세포에 대한 증식억제 활성은 250 μg/mL농도에서 각각 76.26%, 54.59% 및 43.52%로 추출물 중 가장 높은 활성을 보였다. 5. 개똥쑥 추출물로부터 HPLC를 이용하여 phenolic acid, flavonol 및 catechin을 분석한 결과, phenolic acid는 잎의 물 및 에탄올 추출물에서 각각 10종 및 9종, 줄기 추출물에서 각각 8종 및 6종이 동정되었다. Flavonol류는 잎에서만 동정되었으며, rutin 과 kaempferol은 잎 에탄올 추출물에서만 동정되었다. Catechin류는 줄기에 비해 잎에서 거의 모든 화합물이 훨씬 높게 정량되었으며 특히, 잎 에탄올 추출물은 catechin, gallocatechin gallate, epicatechin gallate 및 catechin gallate의 함량이 월등히 높았다.
Gaeddongssuk (Artemisia annua L.), also called Janipssuk grows by the roadside, in the empty space, and on the riverside, etc. and reaches up to about 1 meter in height. Gaeddongssuk, which has greenish yellow flowers from June to August, is widely distributed in China, Europe and America as well as...
Gaeddongssuk (Artemisia annua L.), also called Janipssuk grows by the roadside, in the empty space, and on the riverside, etc. and reaches up to about 1 meter in height. Gaeddongssuk, which has greenish yellow flowers from June to August, is widely distributed in China, Europe and America as well as in Korea (especially in Kyeonggi, Cheju, Pyeongbuk, and Hamnam Province). In China, Gaeddongssuk has been considered medicinal herb for malaria. As artemisinin, a major compound of sesquiterpene, has strong antimalarial effect, it is used as medicines nowadays. The essential oils of Gaeddongssuk are known to be linalool, 1,8-cineol, ρ-cymene, thuione, and camphor, etc., while very few investigations on Gaeddongssuk have been done in our country. The nutritional properties and biological activities of leaves and stems from Gaeddongssuk were investigated. Water and 80% ethanol were extracted from both its leaves and stems. Antioxidant and biological activity of 4 kinds of extracts were measured in vitro. In addition, phenolic compounds from Gaeddongssuk were analyzed through quantitive analysis by HPLC. 1. The content of moisture, crude lipid and crude protein was significantly higher in the leaves, and that of ash and crude fiber was shown significantly higher in the stems of Gaeddongssuk. Nine kinds of minerals were detected in the leaves and six in the stems, and content of K and Ca was highest among the detected minerals. The composition amino acids contents of leaves and stems were 2356.10 mg% and 996.34 mg%, respectively. In other words, composition amino acids content of leaves was over 2 times higher than that of stems. And glutamic acid content was highest of all in the composition amino acids. Free amino acids content of leaves was about 1.4 times higher than that of stems similarly to composition amino acids content. And proline content occupied 50% of total free amino acids. 2. Total phenol content of water and 80% ethanol extract of leaves from Gaeddongssuk was 88.19 mg/g and 99.98 mg/g, and that of water and 80% ethanol extract of stems was 45.03 mg/g and 41.44 mg/g, respectively. Total phenol content of leaves was about 2 times higher than that of stems. The content of flavonoid in ethanol extract made from leaves in Gaeddongssuk was highest with the values of 51.86 mg/g. 3. The antioxidant and biological activities of water and ethanol extracts made from leaves and stems of gaeddongssuk were tested by measuring DPPH (1,1-diphenyl-2-picrylhydrazyl)․ABTS [2,2'-azinobis-(3-ethylbenzo- thiazoline-6-sulfonate)]․NO (nitric oxide) radical scavenging activity, reducing power, FRAP (ferric-reducing antioxidant potential ability) assay, Fe2+ chelating activity, tyrosinase inhibition activity and α-glucosidase inhibition activity. Antioxidant and biological activities were significantly increased in a does-dependent manner, and those of water and ethanol extract of leaves were stronger than those of stems. Especially, DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power and tyrosinase inhibition activity were significantly stronger in leaves extracts than in stems extracts of Gaeddongssuk but unexpectedly, α-glucosidase inhibition activity was stronger in stems extracts. 4. The growth inhibition activity in leaves and stems extracts of Gaeddongssuk on the three human cancer cell lines such as human breast adenocarcinoma cell line MCF-7, human cervix epitheloid carcinoma cell line HeLa and human stomach adenocarcinoma cell line AGS was measured. Growth inhibition activity on human cancer cells was significantly increased in a does-dependent manner. Then leaves and ethanol extracts had stronger activity than stems and water extracts, respectively. Especially, the rates of growth inhibition in ethanol extract of leaves were 76.26%, 54.59% and 43.52% on MCF-7, HeLa and AGS cells, at the concentration of 250 μg/mL, respectively, which were highest of all extracts. 5. The phenolic compounds such as phenolic acid, flavonol and catechin from gaeddongssuk extracts were determined by HPLC. Water and ethanol extract of leaves were identified to have 10 kinds and 9 kinds of phenolic acid, respectively. Water and ethanol extract of stems were identified to have 8 and 6 kinds of phenolic acid, respectively. Flavonoid was identified only in leaves, and rutin and kaempferol were identified only in ethanol extract of leaves. Catechin content of leaves extract was detected higher than that of stems extract. Especially, the contents of catechin, gallocatechin gallate, epicatechin gallate and catechin gallate were significantly higher in ethanol extract of leaves.
Gaeddongssuk (Artemisia annua L.), also called Janipssuk grows by the roadside, in the empty space, and on the riverside, etc. and reaches up to about 1 meter in height. Gaeddongssuk, which has greenish yellow flowers from June to August, is widely distributed in China, Europe and America as well as in Korea (especially in Kyeonggi, Cheju, Pyeongbuk, and Hamnam Province). In China, Gaeddongssuk has been considered medicinal herb for malaria. As artemisinin, a major compound of sesquiterpene, has strong antimalarial effect, it is used as medicines nowadays. The essential oils of Gaeddongssuk are known to be linalool, 1,8-cineol, ρ-cymene, thuione, and camphor, etc., while very few investigations on Gaeddongssuk have been done in our country. The nutritional properties and biological activities of leaves and stems from Gaeddongssuk were investigated. Water and 80% ethanol were extracted from both its leaves and stems. Antioxidant and biological activity of 4 kinds of extracts were measured in vitro. In addition, phenolic compounds from Gaeddongssuk were analyzed through quantitive analysis by HPLC. 1. The content of moisture, crude lipid and crude protein was significantly higher in the leaves, and that of ash and crude fiber was shown significantly higher in the stems of Gaeddongssuk. Nine kinds of minerals were detected in the leaves and six in the stems, and content of K and Ca was highest among the detected minerals. The composition amino acids contents of leaves and stems were 2356.10 mg% and 996.34 mg%, respectively. In other words, composition amino acids content of leaves was over 2 times higher than that of stems. And glutamic acid content was highest of all in the composition amino acids. Free amino acids content of leaves was about 1.4 times higher than that of stems similarly to composition amino acids content. And proline content occupied 50% of total free amino acids. 2. Total phenol content of water and 80% ethanol extract of leaves from Gaeddongssuk was 88.19 mg/g and 99.98 mg/g, and that of water and 80% ethanol extract of stems was 45.03 mg/g and 41.44 mg/g, respectively. Total phenol content of leaves was about 2 times higher than that of stems. The content of flavonoid in ethanol extract made from leaves in Gaeddongssuk was highest with the values of 51.86 mg/g. 3. The antioxidant and biological activities of water and ethanol extracts made from leaves and stems of gaeddongssuk were tested by measuring DPPH (1,1-diphenyl-2-picrylhydrazyl)․ABTS [2,2'-azinobis-(3-ethylbenzo- thiazoline-6-sulfonate)]․NO (nitric oxide) radical scavenging activity, reducing power, FRAP (ferric-reducing antioxidant potential ability) assay, Fe2+ chelating activity, tyrosinase inhibition activity and α-glucosidase inhibition activity. Antioxidant and biological activities were significantly increased in a does-dependent manner, and those of water and ethanol extract of leaves were stronger than those of stems. Especially, DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power and tyrosinase inhibition activity were significantly stronger in leaves extracts than in stems extracts of Gaeddongssuk but unexpectedly, α-glucosidase inhibition activity was stronger in stems extracts. 4. The growth inhibition activity in leaves and stems extracts of Gaeddongssuk on the three human cancer cell lines such as human breast adenocarcinoma cell line MCF-7, human cervix epitheloid carcinoma cell line HeLa and human stomach adenocarcinoma cell line AGS was measured. Growth inhibition activity on human cancer cells was significantly increased in a does-dependent manner. Then leaves and ethanol extracts had stronger activity than stems and water extracts, respectively. Especially, the rates of growth inhibition in ethanol extract of leaves were 76.26%, 54.59% and 43.52% on MCF-7, HeLa and AGS cells, at the concentration of 250 μg/mL, respectively, which were highest of all extracts. 5. The phenolic compounds such as phenolic acid, flavonol and catechin from gaeddongssuk extracts were determined by HPLC. Water and ethanol extract of leaves were identified to have 10 kinds and 9 kinds of phenolic acid, respectively. Water and ethanol extract of stems were identified to have 8 and 6 kinds of phenolic acid, respectively. Flavonoid was identified only in leaves, and rutin and kaempferol were identified only in ethanol extract of leaves. Catechin content of leaves extract was detected higher than that of stems extract. Especially, the contents of catechin, gallocatechin gallate, epicatechin gallate and catechin gallate were significantly higher in ethanol extract of leaves.
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.