The overproduction of free radicals can cause extensive damage to tissues and biomolecules, leading to various pathological conditions and degenerative diseases. Many synthetic drugs with antioxidative effect can protect against oxidative damage, but the various kinds of adverse side effects are bec...
The overproduction of free radicals can cause extensive damage to tissues and biomolecules, leading to various pathological conditions and degenerative diseases. Many synthetic drugs with antioxidative effect can protect against oxidative damage, but the various kinds of adverse side effects are becoming serious issues. So dietary antioxidant intake may be an important strategy for inhibiting or delaying the oxidation of susceptible cellular substrates by overproduction of free radicals, and is thus relevant to disease prevention in many paradigms. This study was designed to investigate the anti-inflammation, anti-aging and protective effect of the neuronal cell by regulation of oxidative stress of perilla frutescens and rosmarinic acid.
The protective effect against 3-morpholinosydnonimine (SIN-1)-induced oxidative stress was investigated using LLC-PK1 porcine renal epithelial cell. The result showed that perilla frutescens and rosmarinic acid increased significantly the cell viability and inhibited lipid peroxidation as does-dependent manner. Moreover, perilla frutescens and rosmarinic acid also have the strong scavenging activity of nitric oxide (NO) generated by SIN-1. The results suggest that perilla frutescens and its active component, rosmarinic acid, would have the protective role against oxidative stress induced by free radical.
In order to investigate the anti-inflammatory activity of perilla frutescens and rosmarinic acid, we employed NO-induced cellular inflammatory system using Raw 264.7 macrophage cell. The results showed that perilla frutescens and rosmarinic acid can scavenge NO generated by both exotoxin sodiumnitroprusside (SNP) and endotoxin lipopolysaccharide (LPS) & interferon-gamma (IFN-γ). In addition, they inhibited lipid peroxidation significantly as does-dependent manner. The results suggest that perilla frutescens would have the protective role against inflammation induced by exotoxin and endotoxin.
In addition, the aging model of H2O2-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of perilla frutescens and rosmarinic acid. H2O2-treated WI-38 cells showed the loss of cell viability, the increase of lipid peroxidation. However, after-treatment with perilla frutescens and rosmarinic acid to WI-38 cells under premature senescence by both acute and chronic oxidative stress could delay the cellular aging process and prevent lipid peroxidation against oxidative stress.
Furthermore, the protective role of perilla frutescens and rosmarinic acid from oxidative stress-induced neuronal cell damage was also studied. The treatment of H2O2 to C6 glial cell induced oxidative stress, however perilla frutescens and rosmarinic acid increased significantly the cell viability and inhibited lipid peroxidation as does-dependent manner. Moreover, perilla frutescens and rosmarinic acid also have the strong scavenging activity of NO generated by LPS & IFN-γ. The results suggest that perilla frutescens and rosmarinic acid would have the protective role of neuronal cell induced by free radical and neuronal toxin.
To study the protective mechanisims from inflammation and oxidative stress of perilla frutescens and rosmarinic acid, the expressions of mRNA and protein, inhibitor κB (IκB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) under cellular oxidative model was investigated. The results showed that LLC-PK1 cell, Raw 264.7 cell and C6 glial cell under oxidative stress increased the expressions of protein and mRNA related to inflammation and oxidative stress. However, the treatments of perilla frutescens and rosmarinic acid treatment led to reduction of protein and mRNA expression of IκB, COX-2 and iNOS. In conclusion, perilla frutescens and rosmarinic acid have anti-inflammation, anti-aging and protective role of neuronal cell through alleviating oxidative stress.
The overproduction of free radicals can cause extensive damage to tissues and biomolecules, leading to various pathological conditions and degenerative diseases. Many synthetic drugs with antioxidative effect can protect against oxidative damage, but the various kinds of adverse side effects are becoming serious issues. So dietary antioxidant intake may be an important strategy for inhibiting or delaying the oxidation of susceptible cellular substrates by overproduction of free radicals, and is thus relevant to disease prevention in many paradigms. This study was designed to investigate the anti-inflammation, anti-aging and protective effect of the neuronal cell by regulation of oxidative stress of perilla frutescens and rosmarinic acid.
The protective effect against 3-morpholinosydnonimine (SIN-1)-induced oxidative stress was investigated using LLC-PK1 porcine renal epithelial cell. The result showed that perilla frutescens and rosmarinic acid increased significantly the cell viability and inhibited lipid peroxidation as does-dependent manner. Moreover, perilla frutescens and rosmarinic acid also have the strong scavenging activity of nitric oxide (NO) generated by SIN-1. The results suggest that perilla frutescens and its active component, rosmarinic acid, would have the protective role against oxidative stress induced by free radical.
In order to investigate the anti-inflammatory activity of perilla frutescens and rosmarinic acid, we employed NO-induced cellular inflammatory system using Raw 264.7 macrophage cell. The results showed that perilla frutescens and rosmarinic acid can scavenge NO generated by both exotoxin sodiumnitroprusside (SNP) and endotoxin lipopolysaccharide (LPS) & interferon-gamma (IFN-γ). In addition, they inhibited lipid peroxidation significantly as does-dependent manner. The results suggest that perilla frutescens would have the protective role against inflammation induced by exotoxin and endotoxin.
In addition, the aging model of H2O2-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of perilla frutescens and rosmarinic acid. H2O2-treated WI-38 cells showed the loss of cell viability, the increase of lipid peroxidation. However, after-treatment with perilla frutescens and rosmarinic acid to WI-38 cells under premature senescence by both acute and chronic oxidative stress could delay the cellular aging process and prevent lipid peroxidation against oxidative stress.
Furthermore, the protective role of perilla frutescens and rosmarinic acid from oxidative stress-induced neuronal cell damage was also studied. The treatment of H2O2 to C6 glial cell induced oxidative stress, however perilla frutescens and rosmarinic acid increased significantly the cell viability and inhibited lipid peroxidation as does-dependent manner. Moreover, perilla frutescens and rosmarinic acid also have the strong scavenging activity of NO generated by LPS & IFN-γ. The results suggest that perilla frutescens and rosmarinic acid would have the protective role of neuronal cell induced by free radical and neuronal toxin.
To study the protective mechanisims from inflammation and oxidative stress of perilla frutescens and rosmarinic acid, the expressions of mRNA and protein, inhibitor κB (IκB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) under cellular oxidative model was investigated. The results showed that LLC-PK1 cell, Raw 264.7 cell and C6 glial cell under oxidative stress increased the expressions of protein and mRNA related to inflammation and oxidative stress. However, the treatments of perilla frutescens and rosmarinic acid treatment led to reduction of protein and mRNA expression of IκB, COX-2 and iNOS. In conclusion, perilla frutescens and rosmarinic acid have anti-inflammation, anti-aging and protective role of neuronal cell through alleviating oxidative stress.
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