To investigate the effects of growth regulating substances on the callus tissues of tree-wounds, the twig segments of 5 kinds of trees including Zelkova serrata, Ziziphus jujuba var. inermis, Pinus koraiensis, Ginkgo biloba. and Aesculus turbinata were cultured on MS-agar medium which were amended w...
To investigate the effects of growth regulating substances on the callus tissues of tree-wounds, the twig segments of 5 kinds of trees including Zelkova serrata, Ziziphus jujuba var. inermis, Pinus koraiensis, Ginkgo biloba. and Aesculus turbinata were cultured on MS-agar medium which were amended with certain concentrations of plant hormones and callus formation was evaluated. From this experiment, some kinds of auxins and cytokinins were selected and the callus inducing agents were formulated. The callus inducing agents were pasted on the artificial-wounds on stems of 4 kinds of trees including Zelkova serrata, Ziziphus jujuba var. inermis, Pinus koraiensis, Ginkgo biloba and the sizes of newly formed callus tissues and the electrical resistance of cambial area around the wound were measured periodically. As a result of inoculating auxin and cytokinin hormone-ameded MS medium to the stem section of each tree, callus was induced in the formation layer of Zelkova serrata and Pinus densiflora from 4 weeks after inoculation. As results of conducting a 4-week dark-culture with the MS medium processed with auxin and cytokinin of five concentration levels, 0.5, 1, 2, 4 and 8 mg/L, Zelkova serrata formed callus by 2,4-D and NAA of auxin, and the highest callus-formation rate was recorded in NAA 2mg/L. It was also found that the most appropriate hormone for the callus induction of Pinus densiflora was 2,4-D 1mg/L and NAA 8mg/L. As a result of applying the callus inducing agent to two kinds of broad-leaved trees, Zelkova serrata and Acer palmatum at different concentration rates of plant hormones, according to the result of in-vitro callus induction, Zelkova serrata was thought to be more sensitive because it showed 14.56 ㎜ of callus formation during 12 weeks in the Topsin-paste treatment, used as a control group. Acer palmatum showed 10.88 ㎜, the highest formation rate at 2,4-D 2 mg/L. The results of the callus inducing agent applicationss to two kinds of needle-leaved trees, Pinus koraiensis and Ginkgo biloba, at different hormone concentration rates were as follows. Pinus koraiensis showed 10.88 ㎜, the highest formation rate at NAA 8 mg/L. On the other hand, Ginkgo biloba showed 4.51 ㎜ at IBA 1 mg/L, which was the lowest formation rate out of all treatments among the trees. It was found that all the four kinds of trees had decay in the wound area treated with vaseline, and it was also found that there remained oily residue in the applied area till 10 weeks after application. Electric resistance of cambial area around the wounds changed variously according to the season and the kinds of host plants. Broad-leaved trees showed a large degree of variations in each season, while Pinus koraiensis showed just a small range of variations in its growth period from July or August to October. The callus tissues were formed faster and better in the wounds in the tissues showing more vitality since the electric resistance of cambial area was low. In the vaseline treatment which the wounded area was decayed, there was an extremely low electrical resistance measurement. In addition, in the treatment that the wounded area was dried up and the callus tissue was not formed, high electrical resistances were measured. It could be said that the callus was not likely to be formed due to the low vital degree. As results of these researches, 2,4-D and NAA showed strong effects on callus formation and they could be developed to the callus inducing agents.
To investigate the effects of growth regulating substances on the callus tissues of tree-wounds, the twig segments of 5 kinds of trees including Zelkova serrata, Ziziphus jujuba var. inermis, Pinus koraiensis, Ginkgo biloba. and Aesculus turbinata were cultured on MS-agar medium which were amended with certain concentrations of plant hormones and callus formation was evaluated. From this experiment, some kinds of auxins and cytokinins were selected and the callus inducing agents were formulated. The callus inducing agents were pasted on the artificial-wounds on stems of 4 kinds of trees including Zelkova serrata, Ziziphus jujuba var. inermis, Pinus koraiensis, Ginkgo biloba and the sizes of newly formed callus tissues and the electrical resistance of cambial area around the wound were measured periodically. As a result of inoculating auxin and cytokinin hormone-ameded MS medium to the stem section of each tree, callus was induced in the formation layer of Zelkova serrata and Pinus densiflora from 4 weeks after inoculation. As results of conducting a 4-week dark-culture with the MS medium processed with auxin and cytokinin of five concentration levels, 0.5, 1, 2, 4 and 8 mg/L, Zelkova serrata formed callus by 2,4-D and NAA of auxin, and the highest callus-formation rate was recorded in NAA 2mg/L. It was also found that the most appropriate hormone for the callus induction of Pinus densiflora was 2,4-D 1mg/L and NAA 8mg/L. As a result of applying the callus inducing agent to two kinds of broad-leaved trees, Zelkova serrata and Acer palmatum at different concentration rates of plant hormones, according to the result of in-vitro callus induction, Zelkova serrata was thought to be more sensitive because it showed 14.56 ㎜ of callus formation during 12 weeks in the Topsin-paste treatment, used as a control group. Acer palmatum showed 10.88 ㎜, the highest formation rate at 2,4-D 2 mg/L. The results of the callus inducing agent applicationss to two kinds of needle-leaved trees, Pinus koraiensis and Ginkgo biloba, at different hormone concentration rates were as follows. Pinus koraiensis showed 10.88 ㎜, the highest formation rate at NAA 8 mg/L. On the other hand, Ginkgo biloba showed 4.51 ㎜ at IBA 1 mg/L, which was the lowest formation rate out of all treatments among the trees. It was found that all the four kinds of trees had decay in the wound area treated with vaseline, and it was also found that there remained oily residue in the applied area till 10 weeks after application. Electric resistance of cambial area around the wounds changed variously according to the season and the kinds of host plants. Broad-leaved trees showed a large degree of variations in each season, while Pinus koraiensis showed just a small range of variations in its growth period from July or August to October. The callus tissues were formed faster and better in the wounds in the tissues showing more vitality since the electric resistance of cambial area was low. In the vaseline treatment which the wounded area was decayed, there was an extremely low electrical resistance measurement. In addition, in the treatment that the wounded area was dried up and the callus tissue was not formed, high electrical resistances were measured. It could be said that the callus was not likely to be formed due to the low vital degree. As results of these researches, 2,4-D and NAA showed strong effects on callus formation and they could be developed to the callus inducing agents.
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