This aims of this study is to develop Taeksa (Alisma canaliculatum) probiotics as an antibiotic substitutes in the livestock feed. Proper microbial strains were selected in the in vitro trial and in the in vivo trial on pig and Broiler performances were tested. The experimental results can be summar...
This aims of this study is to develop Taeksa (Alisma canaliculatum) probiotics as an antibiotic substitutes in the livestock feed. Proper microbial strains were selected in the in vitro trial and in the in vivo trial on pig and Broiler performances were tested. The experimental results can be summarized as follows. Experiment 1. A Study of the Manufacture of Eco- friendly Feed Additive Using Taeksa for Livestock The present study was conducted to select appropriate microbial strains for Taeksa (Alisma canaliculatum) probiotic production and examine their tolerance to acid, bile, and heat. A total of 16 strains of Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecium, Bacillus subtilis, Bacillus coagulans, and Saccharomyces cerevisiae from Korean Collection for Type Cultures (KCTC) were evaluated in this experiment. The strains were first tested for acid and bile tolerance and the higher tolerance strains were subsequently analyzed for heat tolerance. Among the tested strains, L. acidophilus (KCTC 3111), L. acidophilus (KCTC 3146), L. acidophilus (KCTC 3150), L. plantarum (KCTC 3104), E. faecium (KCTC 2022), B. subtilis (KCTC 3239), B. coagulans (KCTC 1015), S. cerevisiae (KCTC 7915) and S. cerevisiae (KCTC 7928) which displayed a high tolerance to acid and bile were selected for heat tolerance test. L. acidophilus (KCTC 3111), E. faecium (KCTC 2022), B. subtilis (KCTC 3239) and S. cerevisiae (KCTC 7928) which had higher tolerance in acid, bile and heat were finally selected for making fermented Taeksa probiotics. Associative inoculation of Taeksa probiotics with E. coli S93 F5 and S99 LT showed that the numbers of E. coli were increased after 3 hours of inoculation and then decreased gradually compared with the control group of E. coli. In conclusion, the multi-strain Taeksa probiotics tested exhibited a high tolerance to acid, bile, and heat, and had an inhibitory effect on E. coli proliferation. Therefore, it is expected that Taeksa probiotics may provide a viable substitute for antibiotics in livestock feed.
This aims of this study is to develop Taeksa (Alisma canaliculatum) probiotics as an antibiotic substitutes in the livestock feed. Proper microbial strains were selected in the in vitro trial and in the in vivo trial on pig and Broiler performances were tested. The experimental results can be summarized as follows. Experiment 1. A Study of the Manufacture of Eco- friendly Feed Additive Using Taeksa for Livestock The present study was conducted to select appropriate microbial strains for Taeksa (Alisma canaliculatum) probiotic production and examine their tolerance to acid, bile, and heat. A total of 16 strains of Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecium, Bacillus subtilis, Bacillus coagulans, and Saccharomyces cerevisiae from Korean Collection for Type Cultures (KCTC) were evaluated in this experiment. The strains were first tested for acid and bile tolerance and the higher tolerance strains were subsequently analyzed for heat tolerance. Among the tested strains, L. acidophilus (KCTC 3111), L. acidophilus (KCTC 3146), L. acidophilus (KCTC 3150), L. plantarum (KCTC 3104), E. faecium (KCTC 2022), B. subtilis (KCTC 3239), B. coagulans (KCTC 1015), S. cerevisiae (KCTC 7915) and S. cerevisiae (KCTC 7928) which displayed a high tolerance to acid and bile were selected for heat tolerance test. L. acidophilus (KCTC 3111), E. faecium (KCTC 2022), B. subtilis (KCTC 3239) and S. cerevisiae (KCTC 7928) which had higher tolerance in acid, bile and heat were finally selected for making fermented Taeksa probiotics. Associative inoculation of Taeksa probiotics with E. coli S93 F5 and S99 LT showed that the numbers of E. coli were increased after 3 hours of inoculation and then decreased gradually compared with the control group of E. coli. In conclusion, the multi-strain Taeksa probiotics tested exhibited a high tolerance to acid, bile, and heat, and had an inhibitory effect on E. coli proliferation. Therefore, it is expected that Taeksa probiotics may provide a viable substitute for antibiotics in livestock feed.
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