To access T helper 2 cell differentiation effects of IRE on asthma, we administrated the IRE to the OVA-induced asthma model for 4 weeks and examined airway hypersensitivity, concentration of OVA-specific IgE, tissue damage by eosinophile infiltration into the lung, cytokines expression level and th...
To access T helper 2 cell differentiation effects of IRE on asthma, we administrated the IRE to the OVA-induced asthma model for 4 weeks and examined airway hypersensitivity, concentration of OVA-specific IgE, tissue damage by eosinophile infiltration into the lung, cytokines expression level and the changes of immune cell number in Lung, BAL and MLN. Results of this study is as followed; 1. Translocation of GATA-3 prtein, a key transcription factor for Th2 cell differentiation, is reduced by IRE treatment at conc-dependent manner, but not AP-1, NF-kB, and NFAT. 2. In splenocytes, Th2 cytokines such as IL-4, IL-5 and IL-13 were significantly reduced in the IRE-treated groups compare with control groups. 3. Penh values were improved in the all IRE-treated groups compared with control groups and especially showed significant at 50mg/mL. 4. Cell number of eosinophile was significantly reduced in the IRE-treated group compared with control groups, but ratio of monocyte and basophile did not show difference in blood test. 5. Total cell numbers of BAL, MLN, lung and eosinophile number in BAL were significantly reduced in the IRE-treated group compared with control groups. 6. In serum, OVA specific-IgE was significantly reduced in the IRE-treated group compared with control groups. 7. Transcription level of IL-4, IL-13, CCR3 mRNA were reduced in the IRE-treated group compared with control groups. 8. CD4+, CD8+, CD3+/CD69+ lymphocyte numbers in lung, BAL and MLN were significantly reduced in the IRE-treated group compared with control groups, and B220+/CD23+ cells in MLN was also significantly reduced. 9. Histological analysis showed that infiltration of lymphocytes and formation of goblet cells were reduced in the IRE-treated group compared with control groups. In summary, these data represent that IRE potentiates therapeutic activities to the allergic diseases such as asthma by regulating Th2 cell differentiation; Therefor additional study is to need for development of anti-asthma therapeutics based on IRE through multiple and detailed approaches.
To access T helper 2 cell differentiation effects of IRE on asthma, we administrated the IRE to the OVA-induced asthma model for 4 weeks and examined airway hypersensitivity, concentration of OVA-specific IgE, tissue damage by eosinophile infiltration into the lung, cytokines expression level and the changes of immune cell number in Lung, BAL and MLN. Results of this study is as followed; 1. Translocation of GATA-3 prtein, a key transcription factor for Th2 cell differentiation, is reduced by IRE treatment at conc-dependent manner, but not AP-1, NF-kB, and NFAT. 2. In splenocytes, Th2 cytokines such as IL-4, IL-5 and IL-13 were significantly reduced in the IRE-treated groups compare with control groups. 3. Penh values were improved in the all IRE-treated groups compared with control groups and especially showed significant at 50mg/mL. 4. Cell number of eosinophile was significantly reduced in the IRE-treated group compared with control groups, but ratio of monocyte and basophile did not show difference in blood test. 5. Total cell numbers of BAL, MLN, lung and eosinophile number in BAL were significantly reduced in the IRE-treated group compared with control groups. 6. In serum, OVA specific-IgE was significantly reduced in the IRE-treated group compared with control groups. 7. Transcription level of IL-4, IL-13, CCR3 mRNA were reduced in the IRE-treated group compared with control groups. 8. CD4+, CD8+, CD3+/CD69+ lymphocyte numbers in lung, BAL and MLN were significantly reduced in the IRE-treated group compared with control groups, and B220+/CD23+ cells in MLN was also significantly reduced. 9. Histological analysis showed that infiltration of lymphocytes and formation of goblet cells were reduced in the IRE-treated group compared with control groups. In summary, these data represent that IRE potentiates therapeutic activities to the allergic diseases such as asthma by regulating Th2 cell differentiation; Therefor additional study is to need for development of anti-asthma therapeutics based on IRE through multiple and detailed approaches.
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