In order to reconstruct human bone, an experiment was done on the growth of osteoblasts(MC3T3-E1) by coating Crystalline hydroxyapatite (HAp) onto metal porous scaffold. The scaffold, with main components of nickel(Ni), for this experiment was of isotropic structure which was similar to human bone. ...
In order to reconstruct human bone, an experiment was done on the growth of osteoblasts(MC3T3-E1) by coating Crystalline hydroxyapatite (HAp) onto metal porous scaffold. The scaffold, with main components of nickel(Ni), for this experiment was of isotropic structure which was similar to human bone. The samples of the Ni porous scaffold were of 450㎛, 580㎛, 800㎛, 1,200㎛, and 3,000㎛. First, in order to plate the surface of Ni scaffold with gold, electric current of 0.1A and 5V was applied for 4 minutes. Second, the coating of Crystalline HAp onto the gold surface was done by Ca-EDTA chelate method. To prepare the HAp coating solution, each 0.5M of EDTA and Ca(NO3)2·4H2O was melt into KOH 3.5M. And, 0.3M of KH2PO4 was melt. This coating solution was diluted and used as 60%. Reaction temperature was 100℃, and reaction time was 12 hours. Crystalline HAp was coated onto the gold surface. The coated Crystalline HAp was synthesized as a rod type with 5㎛ of length and 0.5 ∼ 1 ㎛ of width. In the experiment of osteoblasts(MC3T3-E1), the cells didn't grow on the gold surface, but only grow on the HAp surface. The samples with pore size of 1,200㎛ and 3,000㎛ showed growing cells on the surface and inside of the scaffold. But, the samples with pore size of 450㎛, 580㎛, and 800㎛ showed growing cells only on the surface, not inside of the scaffold. This way, with metal scaffold which is most similar to the structure of human bone, through gold-plating which is harmless to human body, this experiment could find the coating technology of Crystalline HAp so as to increase the adhesive power of osteoblasts. It could find the fact that the pore size should be maintained at least 1mm or more, in order to make the osteoblasts permeate into the scaffold and grow in vivo. In the following experiments, through animal experiments, it will be continuously studied to increase the strong points of metal porous scaffold, by researching optimal conditions to derive perfect bone by filling the bone into the pore of scaffold with this material.
In order to reconstruct human bone, an experiment was done on the growth of osteoblasts(MC3T3-E1) by coating Crystalline hydroxyapatite (HAp) onto metal porous scaffold. The scaffold, with main components of nickel(Ni), for this experiment was of isotropic structure which was similar to human bone. The samples of the Ni porous scaffold were of 450㎛, 580㎛, 800㎛, 1,200㎛, and 3,000㎛. First, in order to plate the surface of Ni scaffold with gold, electric current of 0.1A and 5V was applied for 4 minutes. Second, the coating of Crystalline HAp onto the gold surface was done by Ca-EDTA chelate method. To prepare the HAp coating solution, each 0.5M of EDTA and Ca(NO3)2·4H2O was melt into KOH 3.5M. And, 0.3M of KH2PO4 was melt. This coating solution was diluted and used as 60%. Reaction temperature was 100℃, and reaction time was 12 hours. Crystalline HAp was coated onto the gold surface. The coated Crystalline HAp was synthesized as a rod type with 5㎛ of length and 0.5 ∼ 1 ㎛ of width. In the experiment of osteoblasts(MC3T3-E1), the cells didn't grow on the gold surface, but only grow on the HAp surface. The samples with pore size of 1,200㎛ and 3,000㎛ showed growing cells on the surface and inside of the scaffold. But, the samples with pore size of 450㎛, 580㎛, and 800㎛ showed growing cells only on the surface, not inside of the scaffold. This way, with metal scaffold which is most similar to the structure of human bone, through gold-plating which is harmless to human body, this experiment could find the coating technology of Crystalline HAp so as to increase the adhesive power of osteoblasts. It could find the fact that the pore size should be maintained at least 1mm or more, in order to make the osteoblasts permeate into the scaffold and grow in vivo. In the following experiments, through animal experiments, it will be continuously studied to increase the strong points of metal porous scaffold, by researching optimal conditions to derive perfect bone by filling the bone into the pore of scaffold with this material.
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