소리쟁이 뿌리의 물 추출물과 여러 유기용매의 추출 분획물을 제조하고, DPPH radical, hydroxyl radical, superoxide radical 소거활성, ferric reducing ...
소리쟁이 뿌리의 물 추출물과 여러 유기용매의 추출 분획물을 제조하고, DPPH radical, hydroxyl radical, superoxide radical 소거활성, ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), 세포 내 활성산소종(reactive oxygen species, ROS) 소거 활성, quinone reductase (QR) 유도활성을 측정하여 항산화 활성을 조사하였다. 총 페놀 함량은 ethyl acetate fraction(EAF)이 83.26 mg gallic acid equivalent/g (mg GAE/g)으로 유의적으로 가장 높았으며, dichloromethane fraction(DCMF, 8.16 mg GAE/g), ethanol extract(EE, 21.84 mg GAE/g), aqueous fraction(AF, 19.79 mg GAE/g), butanol fraction(BF, 19.03 mg GAE/g), 물 추출물(WE, 17.25mg GAE/g), n-hexane fraction (HF, 10.68 mg GAE/g)의 순으로 높았다. 소리쟁이 뿌리의 황산화 활성은 총 페놀 함량과 비례적으로 항산화 활성이 높았다. DPPH 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고, EAF가 유의적으로 가장 높았으며 AF, EE, BF, WE, DCMF, HF 순으로 높았다. Superoxide radical 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고 EAF, DCMF, EE, BF, WE, AF 순으로 높았으며 HF와 ascorbic acid이 가장 낮았다. Hydroxyl radical 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고, DCMF, EAF가 유의적으로 가장 높았으며, BF, EE, WE, AF, HF 순으로 높았다. FRAP은 EAF가 유의적으로 가장 높았으며, EE, AF, DCMF, WE, BF, HF 순으로 높았다. TEAC는 EAF가 유의적으로 가장 높았고, DCMF, EE, AF, BF, WE, HF 순으로 높았다. ORAC는 EAF가 유의적으로 가장 높고 DCMF, EE, WE, HF, AF, BF 순으로 높았다. HepG2 세포에서의 ROS 소거활성은 EAF이 유의적으로 가장 높았으며, HF, BF, AF, DCMF, WE, EE 순으로 높았다. 소리쟁이 뿌리의 추출물과 분획물의 QR 유도활성은 소리쟁이 뿌리 추출물과 분획물에 존재하는 다양한 페놀성 화합물 및 anthraquinone계열 화합물들에 기인하는 것으로 생각된다. 이와 같이 소리쟁이 뿌리 추출물과 분획물은 in-vitro 항산화 방법에서뿐 아니라 HepG2 세포에서도 ROS를 유의적으로 감소시켜 산화적 스트레스를 효과적으로 감소시키는 것으로 확인 되었다.
소리쟁이 뿌리의 물 추출물과 여러 유기용매의 추출 분획물을 제조하고, DPPH radical, hydroxyl radical, superoxide radical 소거활성, ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), 세포 내 활성산소종(reactive oxygen species, ROS) 소거 활성, quinone reductase (QR) 유도활성을 측정하여 항산화 활성을 조사하였다. 총 페놀 함량은 ethyl acetate fraction(EAF)이 83.26 mg gallic acid equivalent/g (mg GAE/g)으로 유의적으로 가장 높았으며, dichloromethane fraction(DCMF, 8.16 mg GAE/g), ethanol extract(EE, 21.84 mg GAE/g), aqueous fraction(AF, 19.79 mg GAE/g), butanol fraction(BF, 19.03 mg GAE/g), 물 추출물(WE, 17.25mg GAE/g), n-hexane fraction (HF, 10.68 mg GAE/g)의 순으로 높았다. 소리쟁이 뿌리의 황산화 활성은 총 페놀 함량과 비례적으로 항산화 활성이 높았다. DPPH 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고, EAF가 유의적으로 가장 높았으며 AF, EE, BF, WE, DCMF, HF 순으로 높았다. Superoxide radical 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고 EAF, DCMF, EE, BF, WE, AF 순으로 높았으며 HF와 ascorbic acid이 가장 낮았다. Hydroxyl radical 소거활성은 추출물과 분획물의 농도에 비례하여 증가하였고, DCMF, EAF가 유의적으로 가장 높았으며, BF, EE, WE, AF, HF 순으로 높았다. FRAP은 EAF가 유의적으로 가장 높았으며, EE, AF, DCMF, WE, BF, HF 순으로 높았다. TEAC는 EAF가 유의적으로 가장 높았고, DCMF, EE, AF, BF, WE, HF 순으로 높았다. ORAC는 EAF가 유의적으로 가장 높고 DCMF, EE, WE, HF, AF, BF 순으로 높았다. HepG2 세포에서의 ROS 소거활성은 EAF이 유의적으로 가장 높았으며, HF, BF, AF, DCMF, WE, EE 순으로 높았다. 소리쟁이 뿌리의 추출물과 분획물의 QR 유도활성은 소리쟁이 뿌리 추출물과 분획물에 존재하는 다양한 페놀성 화합물 및 anthraquinone계열 화합물들에 기인하는 것으로 생각된다. 이와 같이 소리쟁이 뿌리 추출물과 분획물은 in-vitro 항산화 방법에서뿐 아니라 HepG2 세포에서도 ROS를 유의적으로 감소시켜 산화적 스트레스를 효과적으로 감소시키는 것으로 확인 되었다.
This study was carried out to compare the antioxidative effects of various Rumex crispus L. root extracts and organic fractions, including n-hexane (HF), dichloromethane (DCMF), ethyl acetate (EAF), n-butanol (BF), and aqueous fractions (AF), which were obtained from the ethanol extracts of Rumex cr...
This study was carried out to compare the antioxidative effects of various Rumex crispus L. root extracts and organic fractions, including n-hexane (HF), dichloromethane (DCMF), ethyl acetate (EAF), n-butanol (BF), and aqueous fractions (AF), which were obtained from the ethanol extracts of Rumex crispus L. root. Antioxidant activities were measured by the assays of DPPH radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, ferric reducing antioxidant power (FRAP), oxygen radical antioxidant capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), intercellular ROS scavenging, and quinone reductase (QR) inducing activity. Total phenolic contents were the highest with 83.26 mg gallic acid equivalent/g (mg GAE/g)in the EAF and showed in the order of DCMF (8.16 mg GAE/g), EE (21.84 mg GAE/g), AF (19.79 mg GAE/g), BF (19.03 mg GAE/g), WE (17.25mg GAE/g), HF (10.68 mg GAE/g). Strong antioxidant effects of Rumex crispus L. root extract seem to be caused by the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents. DPPH scavenging increased proportional to the concentration of extracts and fractions. EAF showed the highest increase and the rest increased in the order of AF, EE, BF, WE, DCMF, and HF. Superoxide radical scavenging increased proportional to the concentration of extracts and fractions. The scavenging activity increased in the order of EAF, DCMF, EE, BF, WE, AF. HF and ascorbic acid showed the least increase. Hydroxyl radical scavenging increased proportional to the concentration of extracts and fractions. DCMF and EAF showed the highest increase and the rest increased in the order of BF, EE, WE, AF, and HF. EAF showed the highest increase in reducing power and the rest increased in the order of EE, AF, DCMF, WE, BF, and HF. In TEAC, EAF showed the highest increase and the rest increased in the order of DCMF, EE, AF, BF, WE, and HF. In ORAC, EAF showed the highest increase and the rest increased in the order of DCMF, EE, WE, HF, AF, and BF. All extracts and fractions from Rumex crispus L. root efficiently scavenged radicals. Especially, EAF exhibited considerable antioxidant potentials showing strong free radical scavenging activity. In addition, intracellular ROS accumulation resulting from H2O2 treatment of HepG2 cell was significantly reduced when EAF was present in the medium compared to HepG2 cell treated with H2O2. QR inducing activity of extracts and fractions of Rumex crispus L. root was supposed to be due to the phenolic hydroxyl group and anthraquinone with potent QR inducing activity. Thus, antioxidant activities of Rumex crispus L. root extracts and fractions were confirmed that not only the in-vitro anti-oxidation assay, it significantly reduced the ROS in HepG2 cells, thereby effectively reducing the oxidative stress.
This study was carried out to compare the antioxidative effects of various Rumex crispus L. root extracts and organic fractions, including n-hexane (HF), dichloromethane (DCMF), ethyl acetate (EAF), n-butanol (BF), and aqueous fractions (AF), which were obtained from the ethanol extracts of Rumex crispus L. root. Antioxidant activities were measured by the assays of DPPH radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, ferric reducing antioxidant power (FRAP), oxygen radical antioxidant capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), intercellular ROS scavenging, and quinone reductase (QR) inducing activity. Total phenolic contents were the highest with 83.26 mg gallic acid equivalent/g (mg GAE/g)in the EAF and showed in the order of DCMF (8.16 mg GAE/g), EE (21.84 mg GAE/g), AF (19.79 mg GAE/g), BF (19.03 mg GAE/g), WE (17.25mg GAE/g), HF (10.68 mg GAE/g). Strong antioxidant effects of Rumex crispus L. root extract seem to be caused by the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents. DPPH scavenging increased proportional to the concentration of extracts and fractions. EAF showed the highest increase and the rest increased in the order of AF, EE, BF, WE, DCMF, and HF. Superoxide radical scavenging increased proportional to the concentration of extracts and fractions. The scavenging activity increased in the order of EAF, DCMF, EE, BF, WE, AF. HF and ascorbic acid showed the least increase. Hydroxyl radical scavenging increased proportional to the concentration of extracts and fractions. DCMF and EAF showed the highest increase and the rest increased in the order of BF, EE, WE, AF, and HF. EAF showed the highest increase in reducing power and the rest increased in the order of EE, AF, DCMF, WE, BF, and HF. In TEAC, EAF showed the highest increase and the rest increased in the order of DCMF, EE, AF, BF, WE, and HF. In ORAC, EAF showed the highest increase and the rest increased in the order of DCMF, EE, WE, HF, AF, and BF. All extracts and fractions from Rumex crispus L. root efficiently scavenged radicals. Especially, EAF exhibited considerable antioxidant potentials showing strong free radical scavenging activity. In addition, intracellular ROS accumulation resulting from H2O2 treatment of HepG2 cell was significantly reduced when EAF was present in the medium compared to HepG2 cell treated with H2O2. QR inducing activity of extracts and fractions of Rumex crispus L. root was supposed to be due to the phenolic hydroxyl group and anthraquinone with potent QR inducing activity. Thus, antioxidant activities of Rumex crispus L. root extracts and fractions were confirmed that not only the in-vitro anti-oxidation assay, it significantly reduced the ROS in HepG2 cells, thereby effectively reducing the oxidative stress.
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