이 논문은 새로운 미생물제제인 Bacillus subtilis HSB904의 개발 과정과 적용 결과를 보고한 것이다. 먼저 전국의 21개 지역에서 359개의 시료를 채취 하여 1차로 539 균주를 선발하고, 다시 전분, 단백질, 지질을 첨가한 배지에서 배양하여 유기물 분해능이 있는 129 균주를 2차로 선발하였다. 아울러 시들음병균, 탄저병균 외 수종의 식물병원균에 길항능이 있는 균주를 선발하였다. 최종적으로는 유기물 분해능과 길항능을 동시에 갖는 유용 기능성 미생물 균주로 SA-15균주를 선발할 수 있었다. 이 균주는 염기서열분석, 당이용성 분석, 균체지방산 조성 분석결과 Bacillus subtilis 임을 확인 동정하고 HSB904로 명명하였다. 이러한 과정으로 선발된 HSB904 균주를 대상으로 최적 배양조건을 조사하였는데 온도는 30∼35 ℃ pH는 5.5∼7.0가 최적조건임을 알게 되었다. 그리고 이 균주를 이용한 미생물제제를 만들기 위해 필요한 증량제와 ...
이 논문은 새로운 미생물제제인 Bacillus subtilis HSB904의 개발 과정과 적용 결과를 보고한 것이다. 먼저 전국의 21개 지역에서 359개의 시료를 채취 하여 1차로 539 균주를 선발하고, 다시 전분, 단백질, 지질을 첨가한 배지에서 배양하여 유기물 분해능이 있는 129 균주를 2차로 선발하였다. 아울러 시들음병균, 탄저병균 외 수종의 식물병원균에 길항능이 있는 균주를 선발하였다. 최종적으로는 유기물 분해능과 길항능을 동시에 갖는 유용 기능성 미생물 균주로 SA-15균주를 선발할 수 있었다. 이 균주는 염기서열분석, 당이용성 분석, 균체지방산 조성 분석결과 Bacillus subtilis 임을 확인 동정하고 HSB904로 명명하였다. 이러한 과정으로 선발된 HSB904 균주를 대상으로 최적 배양조건을 조사하였는데 온도는 30∼35 ℃ pH는 5.5∼7.0가 최적조건임을 알게 되었다. 그리고 이 균주를 이용한 미생물제제를 만들기 위해 필요한 증량제와 계면 활성제를 결정하고 제형화시 균주의 보존력과 발아시험을 실시하여 최종적인 제형조건을 제시하였다. 완성된 제형물 미생물제제 HSB904를 벼와 상추 포장에 각각 적용해 본 결과 재배토양의 물리화학성에는 큰 변화를 주지 않았고 토양 미생물상 변화에도 거의 영향을 미치지 않았다. 그러면서 작물의 생육과 수량증대에 영향을 미쳐 상추의 경우 수량이 10∼15% 정도 증가하는 것으로 나타났다. 이 연구에서 개발된 HSB904는 기능성 미생물제제로 토양 유기물분해능과 식물병원균에 대한 길항력을 갖고 있다. 따라서 유기질비료와 함께 시용하면 효과적일 뿐만 아니라 화학비료의 사용량을 줄일 수 있고, 동시에 토양전염성 병원균의 증식을 억제할 수 있다는 점에서 친환경농자재로 쓰일 수 있을 것으로 보이며, 금후 농가소득 증대와 친환경농업 생산시스템 확립에 크게 기여할 것으로 기대된다.
이 논문은 새로운 미생물제제인 Bacillus subtilis HSB904의 개발 과정과 적용 결과를 보고한 것이다. 먼저 전국의 21개 지역에서 359개의 시료를 채취 하여 1차로 539 균주를 선발하고, 다시 전분, 단백질, 지질을 첨가한 배지에서 배양하여 유기물 분해능이 있는 129 균주를 2차로 선발하였다. 아울러 시들음병균, 탄저병균 외 수종의 식물병원균에 길항능이 있는 균주를 선발하였다. 최종적으로는 유기물 분해능과 길항능을 동시에 갖는 유용 기능성 미생물 균주로 SA-15균주를 선발할 수 있었다. 이 균주는 염기서열분석, 당이용성 분석, 균체지방산 조성 분석결과 Bacillus subtilis 임을 확인 동정하고 HSB904로 명명하였다. 이러한 과정으로 선발된 HSB904 균주를 대상으로 최적 배양조건을 조사하였는데 온도는 30∼35 ℃ pH는 5.5∼7.0가 최적조건임을 알게 되었다. 그리고 이 균주를 이용한 미생물제제를 만들기 위해 필요한 증량제와 계면 활성제를 결정하고 제형화시 균주의 보존력과 발아시험을 실시하여 최종적인 제형조건을 제시하였다. 완성된 제형물 미생물제제 HSB904를 벼와 상추 포장에 각각 적용해 본 결과 재배토양의 물리화학성에는 큰 변화를 주지 않았고 토양 미생물상 변화에도 거의 영향을 미치지 않았다. 그러면서 작물의 생육과 수량증대에 영향을 미쳐 상추의 경우 수량이 10∼15% 정도 증가하는 것으로 나타났다. 이 연구에서 개발된 HSB904는 기능성 미생물제제로 토양 유기물분해능과 식물병원균에 대한 길항력을 갖고 있다. 따라서 유기질비료와 함께 시용하면 효과적일 뿐만 아니라 화학비료의 사용량을 줄일 수 있고, 동시에 토양전염성 병원균의 증식을 억제할 수 있다는 점에서 친환경농자재로 쓰일 수 있을 것으로 보이며, 금후 농가소득 증대와 친환경농업 생산시스템 확립에 크게 기여할 것으로 기대된다.
The study has utilized functions, in which accelerate the degradation of organic matters and have the antagonism against the crops diseases, of useful microbe resources for establishing a dosage formation of eco-friendly microbial agents with not only the acceleration of the crops growth but also th...
The study has utilized functions, in which accelerate the degradation of organic matters and have the antagonism against the crops diseases, of useful microbe resources for establishing a dosage formation of eco-friendly microbial agents with not only the acceleration of the crops growth but also the improvement of the soil ecosystem. Furthermore, the study shows that results of effects on crops and soils by using eco-friendly microbial agents are as follows. 1. The sum of 359 samples had been collected from 21 regions, and 129 strains had shown the degradability of organic compounds out of selected and isolated 539 strains as of the first selection. Among the first selected microbial strains with the degradability of organic matters, the second strains had been selected from the first selected microbial strains with the degradability of organic matters by using mediums with starch, protein, and lipid respectively. 2. The degradability of organic matters has been examined and selected for the production of protease, cellulase, xylanase, and amylase, and for the transition of mineral nitrogen to organonitrogen, and the antagonism has been examined against crop diseases such as Fusarium oxysporum, F. solani, Botrytis cinerea, Phytophthora capsici, P. cactorum, Colletotrichum gloeosporioides, Rhizoctonia solani, Scleotinia sclerotiorum, Cylindrocarpon destructans, Pythium ultimum. 3. The final selected strains, which has shown excellent abilities in the degradability of organic matters and the antagonism, is a SA-15 strain, and it has identified as Bacillus subtilis and named as "HSB904" based on the analysis of 16S rRNA base sequence, cellular fatty acid Methyl Esters, and saccharine availability. 4. In order to cultivate a selected HSB904 strain, the optimal medium conditions have been identified, and the optimal temperature and PH have been searched as 30～35℃, 5.5～7.0 respectively. 5. When used in the surfactant at the time of selection microbial culture combination of the NK-NX150 of excellent wettability and NK-VN of excellent dispersible, the first powder formulation raw material was used as selecting. Because extenders and carrier can quickly and evenly distributed. Granular formulation and wettable powders that satisfy the condition of kaolin and bentonite, and materials for the final formulation was confirmed by machine for preparing microbial products and operating conditions are equally and uniformly applied to produce the final parent material, When a mixture of 65% kaolin and 35% bentonite were able to get the best formulation. Finally, 15% to 20% of the molasses solution was the maximum production conditions of granular formulation and the preservation and plant germination tests have been conducted on a dosage formation to establish the final dosage formation conditions. 6. After the final completion of HSB904 strain, the result of packing cultivation test on rice and lettuce has shown that the effects on physico-chemical is moderate. However, it has shown large effects on the growth and quantity increase when the fertilizer and microbial agents are used in combination. In the primary cultivation test on lettuce, the individual weight resulted in 11∼14% increase and the quantity resulted 13∼15% increase on HSB904 treated group against the comparison group. After the statistical analysis, the result of HSB904 treated group against the comparison group in quantity and individual weight, has acknowledged its relationship. 7. In order to find the deviation of soil microorganism by the external microbial agents injection, the soil has been treated with a HSB904 agent. The level of bacteria, a filamentous fungus, and an actinomyces in early stage has shown a little increase, but it has been remained in the same density after 10 days comparing to the comparison group so that it seemed the deviation of soil microorganism by the external microbial agents injection is not effective.
The study has utilized functions, in which accelerate the degradation of organic matters and have the antagonism against the crops diseases, of useful microbe resources for establishing a dosage formation of eco-friendly microbial agents with not only the acceleration of the crops growth but also the improvement of the soil ecosystem. Furthermore, the study shows that results of effects on crops and soils by using eco-friendly microbial agents are as follows. 1. The sum of 359 samples had been collected from 21 regions, and 129 strains had shown the degradability of organic compounds out of selected and isolated 539 strains as of the first selection. Among the first selected microbial strains with the degradability of organic matters, the second strains had been selected from the first selected microbial strains with the degradability of organic matters by using mediums with starch, protein, and lipid respectively. 2. The degradability of organic matters has been examined and selected for the production of protease, cellulase, xylanase, and amylase, and for the transition of mineral nitrogen to organonitrogen, and the antagonism has been examined against crop diseases such as Fusarium oxysporum, F. solani, Botrytis cinerea, Phytophthora capsici, P. cactorum, Colletotrichum gloeosporioides, Rhizoctonia solani, Scleotinia sclerotiorum, Cylindrocarpon destructans, Pythium ultimum. 3. The final selected strains, which has shown excellent abilities in the degradability of organic matters and the antagonism, is a SA-15 strain, and it has identified as Bacillus subtilis and named as "HSB904" based on the analysis of 16S rRNA base sequence, cellular fatty acid Methyl Esters, and saccharine availability. 4. In order to cultivate a selected HSB904 strain, the optimal medium conditions have been identified, and the optimal temperature and PH have been searched as 30～35℃, 5.5～7.0 respectively. 5. When used in the surfactant at the time of selection microbial culture combination of the NK-NX150 of excellent wettability and NK-VN of excellent dispersible, the first powder formulation raw material was used as selecting. Because extenders and carrier can quickly and evenly distributed. Granular formulation and wettable powders that satisfy the condition of kaolin and bentonite, and materials for the final formulation was confirmed by machine for preparing microbial products and operating conditions are equally and uniformly applied to produce the final parent material, When a mixture of 65% kaolin and 35% bentonite were able to get the best formulation. Finally, 15% to 20% of the molasses solution was the maximum production conditions of granular formulation and the preservation and plant germination tests have been conducted on a dosage formation to establish the final dosage formation conditions. 6. After the final completion of HSB904 strain, the result of packing cultivation test on rice and lettuce has shown that the effects on physico-chemical is moderate. However, it has shown large effects on the growth and quantity increase when the fertilizer and microbial agents are used in combination. In the primary cultivation test on lettuce, the individual weight resulted in 11∼14% increase and the quantity resulted 13∼15% increase on HSB904 treated group against the comparison group. After the statistical analysis, the result of HSB904 treated group against the comparison group in quantity and individual weight, has acknowledged its relationship. 7. In order to find the deviation of soil microorganism by the external microbial agents injection, the soil has been treated with a HSB904 agent. The level of bacteria, a filamentous fungus, and an actinomyces in early stage has shown a little increase, but it has been remained in the same density after 10 days comparing to the comparison group so that it seemed the deviation of soil microorganism by the external microbial agents injection is not effective.
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