Objectives : This study was carried out to find out the Antioxidant and Anti-inflammatory Effects of Components of Mahwangbujaseshin-tang through Heme Oxygenase-1 Induction in LPS-Stimulated RAW264.7 Macrophages. Methods : There are 5 experimental groups. ; normal, control, EH(Ephedrae Herba), ALRP(...
Objectives : This study was carried out to find out the Antioxidant and Anti-inflammatory Effects of Components of Mahwangbujaseshin-tang through Heme Oxygenase-1 Induction in LPS-Stimulated RAW264.7 Macrophages. Methods : There are 5 experimental groups. ; normal, control, EH(Ephedrae Herba), ALRP(Aconiti Lateralis Radix Preparata) and AR(Asiasari Radix). The extract of EH(Ephedrae Herba), ALRP(Aconiti Lateralis Radix Preparata) and AR(Asiasari Radix)(100 ㎍/㎖) was added to each group. We examined cytotoxicity, total phenolic contents, DPPH and ABTS free radical scavenging activity, Intracellular ROS(reactive oxygen species) production, NO(Total Nitric oxide), iNOS(inducible nitric oxide synthase), PGE2(prostaglandin E2), COX-2(cyclooxygenase-2), IL-1β(interleukin-1β), IL-6(interleukin-6), TNF-α(tumor necrosis factor-α), MMP-9(matrix metalloproteinase-9), TIMP-1(tissue inhibitor of metalloproteinase-1) and HO-1(heme oxygenase-1) expression level. In addition, we examined the involvement of HO-1 in the inhibitory activities of EH, ALRP, AR extract. Results : 1. Total phenolic contents of EH were in the highest level. 2. DPPH and ABTS free radical scavenging activity of EH was in the highest level. 3. ROS production was significantly decreased in AR. 4. NO production was significantly decreased in EH, ALRP, AR and iNOS expression was decreased in EH, AR. 5. PGE2 production was significantly decreased in EH, AR and COX-2 expression was decreased in EH, AR. 6. IL-1β production was significantly decreased in EH, AR and IL-6 production was significantly decreased in AR. TNF-α production was significantly decreased in ALRP, AR. 7. MMP-9 and TIMP-1 production were significantly decreased in EH. 8. HO-1 expression was significantly increased in EH and slightly increased in ALRP. 9. With simultaneous usage of SnPP which is expression inhibitor of HO-1, NO, IL-1β, IL-6 and TNF-α production were partially increased in EH, ALRP, AR. Conclusions : According to this study, Components of Mahwangbujaseshin-tang have anti-oxidants and anti-inflammation effects in LPS-Stimulated RAW264.7 Macrophages. Key words : Mahwangbujaseshin-tang, Anti-inflammation, Anti-oxidation, Heme oxygenase-1
Objectives : This study was carried out to find out the Antioxidant and Anti-inflammatory Effects of Components of Mahwangbujaseshin-tang through Heme Oxygenase-1 Induction in LPS-Stimulated RAW264.7 Macrophages. Methods : There are 5 experimental groups. ; normal, control, EH(Ephedrae Herba), ALRP(Aconiti Lateralis Radix Preparata) and AR(Asiasari Radix). The extract of EH(Ephedrae Herba), ALRP(Aconiti Lateralis Radix Preparata) and AR(Asiasari Radix)(100 ㎍/㎖) was added to each group. We examined cytotoxicity, total phenolic contents, DPPH and ABTS free radical scavenging activity, Intracellular ROS(reactive oxygen species) production, NO(Total Nitric oxide), iNOS(inducible nitric oxide synthase), PGE2(prostaglandin E2), COX-2(cyclooxygenase-2), IL-1β(interleukin-1β), IL-6(interleukin-6), TNF-α(tumor necrosis factor-α), MMP-9(matrix metalloproteinase-9), TIMP-1(tissue inhibitor of metalloproteinase-1) and HO-1(heme oxygenase-1) expression level. In addition, we examined the involvement of HO-1 in the inhibitory activities of EH, ALRP, AR extract. Results : 1. Total phenolic contents of EH were in the highest level. 2. DPPH and ABTS free radical scavenging activity of EH was in the highest level. 3. ROS production was significantly decreased in AR. 4. NO production was significantly decreased in EH, ALRP, AR and iNOS expression was decreased in EH, AR. 5. PGE2 production was significantly decreased in EH, AR and COX-2 expression was decreased in EH, AR. 6. IL-1β production was significantly decreased in EH, AR and IL-6 production was significantly decreased in AR. TNF-α production was significantly decreased in ALRP, AR. 7. MMP-9 and TIMP-1 production were significantly decreased in EH. 8. HO-1 expression was significantly increased in EH and slightly increased in ALRP. 9. With simultaneous usage of SnPP which is expression inhibitor of HO-1, NO, IL-1β, IL-6 and TNF-α production were partially increased in EH, ALRP, AR. Conclusions : According to this study, Components of Mahwangbujaseshin-tang have anti-oxidants and anti-inflammation effects in LPS-Stimulated RAW264.7 Macrophages. Key words : Mahwangbujaseshin-tang, Anti-inflammation, Anti-oxidation, Heme oxygenase-1
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