This study was conducted to develop efficient fermentation techniques for the production of bioethanol from potato plant, which was composed of starchy tubers and cellulosic by-products such as leaves, stems, and roots. Firstly, the contents of starch, moisture, crude protein, crude fat, crude fiber...
This study was conducted to develop efficient fermentation techniques for the production of bioethanol from potato plant, which was composed of starchy tubers and cellulosic by-products such as leaves, stems, and roots. Firstly, the contents of starch, moisture, crude protein, crude fat, crude fiber, and ash of different varieties of potato tubers were analyzed. The effectiveness of the contents of the different components of the potato tubers on the ethanol production were examined. The results showed that the starch content positively affected the ethanol production. while moisture content affected negatively the ethanol production. Among the several varieties of potato tubers, Ha-ryoung produced the highest yield of ethanol as much as 94±1.9 L/ton or 3111±62.7 L/ha. Secondly, high concentration of ethanol was produced from potato tubers with high content of solid. Potato tubers were ground without addition of water and the viscosity of the potato mash with solid content of 28% was reduced by using 0.1% Viscozyme at 50℃ for 30 min. The potato mash was then liquefied using 0.1% Liquozyme at 90℃ for 30 min and optimal conditions for the simultaneous saccharification and fermentation (SSF) of the potato mash for ethanol production were investigated using statistical methods. Using 24 factorial design, saccharifying-enzyme dosage and incubation temperature were determined to be 1.45 AGU/g dry matter and 31.3℃, respectively. Under these optimal conditions for SSF, 14.92% (v/v) ethanol with 91.0% of theoretical yield was produced after 60 h, and all the starch was completely used up. For the further improvement of SSF process, a thermotolerant yeast strain was developed, since the optimal temperature for saccharifying enzyme is much higher than that of yeast. for efficient ethanol fermentation by SSF. Among various thermotolerent yeast strains, Saccharomyces cerevisiae HT44 strain was selected and this strain produced 15.25% (v/v) ethanol at 38℃ while ATCC26603 strain produced 13.44% (v/v) ethanol. Thirdly, optimal pretreatment condition of cellulosic by-products of potato plant was examined for ethanol production. The optimal pretreatment condition was found to be 1.0 M NaOH, reaction temperature 150℃, and reaction time 30 min. From the pretreated by-products with 10% solid content, 17.79±0.05 g ethanol /L was produced with 91.73% of theroretical yield by HT44 strain.
This study was conducted to develop efficient fermentation techniques for the production of bioethanol from potato plant, which was composed of starchy tubers and cellulosic by-products such as leaves, stems, and roots. Firstly, the contents of starch, moisture, crude protein, crude fat, crude fiber, and ash of different varieties of potato tubers were analyzed. The effectiveness of the contents of the different components of the potato tubers on the ethanol production were examined. The results showed that the starch content positively affected the ethanol production. while moisture content affected negatively the ethanol production. Among the several varieties of potato tubers, Ha-ryoung produced the highest yield of ethanol as much as 94±1.9 L/ton or 3111±62.7 L/ha. Secondly, high concentration of ethanol was produced from potato tubers with high content of solid. Potato tubers were ground without addition of water and the viscosity of the potato mash with solid content of 28% was reduced by using 0.1% Viscozyme at 50℃ for 30 min. The potato mash was then liquefied using 0.1% Liquozyme at 90℃ for 30 min and optimal conditions for the simultaneous saccharification and fermentation (SSF) of the potato mash for ethanol production were investigated using statistical methods. Using 24 factorial design, saccharifying-enzyme dosage and incubation temperature were determined to be 1.45 AGU/g dry matter and 31.3℃, respectively. Under these optimal conditions for SSF, 14.92% (v/v) ethanol with 91.0% of theoretical yield was produced after 60 h, and all the starch was completely used up. For the further improvement of SSF process, a thermotolerant yeast strain was developed, since the optimal temperature for saccharifying enzyme is much higher than that of yeast. for efficient ethanol fermentation by SSF. Among various thermotolerent yeast strains, Saccharomyces cerevisiae HT44 strain was selected and this strain produced 15.25% (v/v) ethanol at 38℃ while ATCC26603 strain produced 13.44% (v/v) ethanol. Thirdly, optimal pretreatment condition of cellulosic by-products of potato plant was examined for ethanol production. The optimal pretreatment condition was found to be 1.0 M NaOH, reaction temperature 150℃, and reaction time 30 min. From the pretreated by-products with 10% solid content, 17.79±0.05 g ethanol /L was produced with 91.73% of theroretical yield by HT44 strain.
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