Essential oil (EO) is hydrophobic and volatile complex compounds extracted from aromatic plants. EO is widely used in the cosmetic industry, food industry, pharmaceutical industry as active components of medicines, ingredients of fragrances, flavors. Recently, as a human-friendly natural resource is...
Essential oil (EO) is hydrophobic and volatile complex compounds extracted from aromatic plants. EO is widely used in the cosmetic industry, food industry, pharmaceutical industry as active components of medicines, ingredients of fragrances, flavors. Recently, as a human-friendly natural resource is receiving much recognition internationally, the natural aromatics resource market is growing fast at korea and abroad. Natural aromatics resource-related research need a high professionalism and technology. But now our country is high dependent on import due to lack of natural aromatics resource-related researcher and technology. Chapter 1. In the present study, the essential oil or absolute (ABS) isolated from 14 species korean indigenous plants and determined their biological activity. Artemisia annua (AA), Artemisia montana PAMPAN (AMP), Chrysanthemum boreale MAKINO(CBM), Chrysanthemum zawadskii var. latilobum (CZVL), Wisteria floribunda (WF), Robinia pseudoacacia L (RPL), Albizzia julibrissin Durazz (AJD), Ligustrum obtusifolium (LO), Chionanthus retusus Lindlley & Paxton (CRLP), Thuja orientalis (TOR), Thuja occidentalis (TOC), Elsholtza splendens (ES) were harvested at Asan campus, Hoseo University, and Pueraria thunbergiana BENTH (PTBF), Syringa dilatata Nakai (SDN) were harvested at mountains, Chen-an/Asan, Chung-nam, Korea. 14 species aromatic plants were identified by Korean National Arboretum and professor Hwan Myung lee. Essential oil or absolute was extracted from 14 species aromatic plants by steam distillation or solvent extraction, and identified the their composition and character by gas chromatography-mass spectrometry. The yields (v/w or w/w%) and compounds of CBMEO in the order of: AAEO (0.5%, 35 compounds), AMPEO (0.25%, 42 compounds), CBMEO (0.25%, 34 compounds), CZVLEO (0.006%, 11 compounds), WFABS (0.05%, 9 compounds), RPLABS (0.014%, 10 compounds), AJDABS (0.08%, 11 compounds), PTBFABS (0.012%, 9 compounds), SDNABS (0.1%, 4 compounds), LOABS (0.45%, 8 compounds), CRLPABS (0.035%, 11 compounds), TOCEO leaf (0.43%, 13 compounds), TOCEO fruit (0.1%, 11 compounds), TOREO leaf (0.037%, 13 compounds), TOREO fruit (0.013%, 14 compounds), ESABS (0.24%, 2 compounds), ESEO (0.15%, 4 compounds). Moreover, biological activity of 17 species EO or ABS was confirmed by literatures search. Chapter 2. To confirm the variation of the chemical composition and skin biological activity of essential oil extracted CBM during growth stage and flowering time, CBM were harvested at Asan campus, Hoseo University at three stage (vegetative, pre-flowering, full-flowering). Essential oil was extracted from 3 species CBM by steam distillation, and identified their composition, skin biological activity(anti-oxidant, whitening effects, skin regeneration). The yields (v/w%) and compounds of CBMEO at different stages were in the order of: vegetative CBMEO (0.092% 37 compounds), pre-flowering CBMEO (0.25%, 34 compounds), full-flowering (0.16%, 33 compounds). Anti-oxidant: The 3 species CBMEO and Eugenol showed the highest DPPH and ABTs radical scavenging capacity in a concentration-dependent manner. Whitening effect: The 3 species CBMEO and Cuminaldehyde inhibits the α-melanocyte stimulating hormone induced-tyrosinase activity and melanin synthesis of B16BL6 melanoma cell in a concentration-dependent manner. Moreover, the 3 species CBMEO induces the phosphorylation of extracellular signal-regulated kinase1/2 (Erk1/2) and p38 MAPK pathway. Skin regeneration: The pre-flowering CBMEO stimulates the migration and proliferation in HaCat, and the phosphorylation of Erk1/2 and serine/threonine-specific protein kinase(Akt) was significantly increased by the pre-flowering CBMEO. In addition, the pre-flowering CBMEO induced the type IV collagen synthesis of HaCat, and promoted wound closure in the dorsal side skin of rat tail. Evaluation of toxicity of CBMEO: CBMEO were showed to be low in toxicity on oral, repeated oral, skin and inhalation toxicity test. Therefore, this study may provide new information for development of a natural aromatics resource industry of Korea.
Essential oil (EO) is hydrophobic and volatile complex compounds extracted from aromatic plants. EO is widely used in the cosmetic industry, food industry, pharmaceutical industry as active components of medicines, ingredients of fragrances, flavors. Recently, as a human-friendly natural resource is receiving much recognition internationally, the natural aromatics resource market is growing fast at korea and abroad. Natural aromatics resource-related research need a high professionalism and technology. But now our country is high dependent on import due to lack of natural aromatics resource-related researcher and technology. Chapter 1. In the present study, the essential oil or absolute (ABS) isolated from 14 species korean indigenous plants and determined their biological activity. Artemisia annua (AA), Artemisia montana PAMPAN (AMP), Chrysanthemum boreale MAKINO(CBM), Chrysanthemum zawadskii var. latilobum (CZVL), Wisteria floribunda (WF), Robinia pseudoacacia L (RPL), Albizzia julibrissin Durazz (AJD), Ligustrum obtusifolium (LO), Chionanthus retusus Lindlley & Paxton (CRLP), Thuja orientalis (TOR), Thuja occidentalis (TOC), Elsholtza splendens (ES) were harvested at Asan campus, Hoseo University, and Pueraria thunbergiana BENTH (PTBF), Syringa dilatata Nakai (SDN) were harvested at mountains, Chen-an/Asan, Chung-nam, Korea. 14 species aromatic plants were identified by Korean National Arboretum and professor Hwan Myung lee. Essential oil or absolute was extracted from 14 species aromatic plants by steam distillation or solvent extraction, and identified the their composition and character by gas chromatography-mass spectrometry. The yields (v/w or w/w%) and compounds of CBMEO in the order of: AAEO (0.5%, 35 compounds), AMPEO (0.25%, 42 compounds), CBMEO (0.25%, 34 compounds), CZVLEO (0.006%, 11 compounds), WFABS (0.05%, 9 compounds), RPLABS (0.014%, 10 compounds), AJDABS (0.08%, 11 compounds), PTBFABS (0.012%, 9 compounds), SDNABS (0.1%, 4 compounds), LOABS (0.45%, 8 compounds), CRLPABS (0.035%, 11 compounds), TOCEO leaf (0.43%, 13 compounds), TOCEO fruit (0.1%, 11 compounds), TOREO leaf (0.037%, 13 compounds), TOREO fruit (0.013%, 14 compounds), ESABS (0.24%, 2 compounds), ESEO (0.15%, 4 compounds). Moreover, biological activity of 17 species EO or ABS was confirmed by literatures search. Chapter 2. To confirm the variation of the chemical composition and skin biological activity of essential oil extracted CBM during growth stage and flowering time, CBM were harvested at Asan campus, Hoseo University at three stage (vegetative, pre-flowering, full-flowering). Essential oil was extracted from 3 species CBM by steam distillation, and identified their composition, skin biological activity(anti-oxidant, whitening effects, skin regeneration). The yields (v/w%) and compounds of CBMEO at different stages were in the order of: vegetative CBMEO (0.092% 37 compounds), pre-flowering CBMEO (0.25%, 34 compounds), full-flowering (0.16%, 33 compounds). Anti-oxidant: The 3 species CBMEO and Eugenol showed the highest DPPH and ABTs radical scavenging capacity in a concentration-dependent manner. Whitening effect: The 3 species CBMEO and Cuminaldehyde inhibits the α-melanocyte stimulating hormone induced-tyrosinase activity and melanin synthesis of B16BL6 melanoma cell in a concentration-dependent manner. Moreover, the 3 species CBMEO induces the phosphorylation of extracellular signal-regulated kinase1/2 (Erk1/2) and p38 MAPK pathway. Skin regeneration: The pre-flowering CBMEO stimulates the migration and proliferation in HaCat, and the phosphorylation of Erk1/2 and serine/threonine-specific protein kinase(Akt) was significantly increased by the pre-flowering CBMEO. In addition, the pre-flowering CBMEO induced the type IV collagen synthesis of HaCat, and promoted wound closure in the dorsal side skin of rat tail. Evaluation of toxicity of CBMEO: CBMEO were showed to be low in toxicity on oral, repeated oral, skin and inhalation toxicity test. Therefore, this study may provide new information for development of a natural aromatics resource industry of Korea.
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