Background: Polymerase chain reaction (PCR) is one of the most commonly used gene analysis techniques which can selectively amplifies DNA template the user required. Recently, PCR technique is expected to be miniaturized due to the development of the micro electro mechanical system (MEMS) technology...
Background: Polymerase chain reaction (PCR) is one of the most commonly used gene analysis techniques which can selectively amplifies DNA template the user required. Recently, PCR technique is expected to be miniaturized due to the development of the micro electro mechanical system (MEMS) technology, and expected to be applied in point of care testing (POCT). Many merits follows such as, conservation of analysis time and less reagent is used in analysis. The problems met in applying PCR in POCT is the high expense of manufacturing tiny thermal conductor due to miniaturization and an external equipment is needed to supply energy. Since more considerations needed an application of the conventional PCR in POCT, our group have manufactured peltier based micro PCR (P-mPCR) device which is mobile, light weight and low cost.
Materials and Methods: In order to control the temperature of the solution in manufactured chip, P-mPCR was manufactured composing of a hot plate, a heat insulation board, a cooling fan, a peltier driver, peltiers and thermistor sensors. In the PCB manufacture, a main-board containing an electric transformer, a voltage regulator, a microprocessor (ATEMEGA128), a LCD board, a peltier driver and A/D converter was used to control temperature. Data of 0.3 second of interval were collected and these data were applied in MATLAB to design algorithm. Also, our group have designed condition of temperature and duration time to apply in both the P-mPCR and the conventional PCR. Both types of PCR performed the same condition of PCR cycle and the outcome was analyzed to investigate the utility of P-mPCR.
Results : For a precise P-mPCR cycle control, our group have investigated the operating preparation time using time-voltage graph, and obtained temperature function using a voltage-temperature graph. We controlled the required voltage of the temperature by investigating temperature function. For utility testing, both conventional and P-mPCR were performed and showed identical data, as a result, we have identified the utility of own invented P-mPCR device.
Conclusions: Our own invented P-mPCR device and a sample channel proved the excellence of PCR and temperature control performance and sample channel used in P-mPCR showed advantage of the mass production according to low cost and disposable use as a bio-chip. After further studies about combination of DNA sample preparation stages and more upgraded chip and device, our group expect this device would be meaningful in gene analysis system market.
Background: Polymerase chain reaction (PCR) is one of the most commonly used gene analysis techniques which can selectively amplifies DNA template the user required. Recently, PCR technique is expected to be miniaturized due to the development of the micro electro mechanical system (MEMS) technology, and expected to be applied in point of care testing (POCT). Many merits follows such as, conservation of analysis time and less reagent is used in analysis. The problems met in applying PCR in POCT is the high expense of manufacturing tiny thermal conductor due to miniaturization and an external equipment is needed to supply energy. Since more considerations needed an application of the conventional PCR in POCT, our group have manufactured peltier based micro PCR (P-mPCR) device which is mobile, light weight and low cost.
Materials and Methods: In order to control the temperature of the solution in manufactured chip, P-mPCR was manufactured composing of a hot plate, a heat insulation board, a cooling fan, a peltier driver, peltiers and thermistor sensors. In the PCB manufacture, a main-board containing an electric transformer, a voltage regulator, a microprocessor (ATEMEGA128), a LCD board, a peltier driver and A/D converter was used to control temperature. Data of 0.3 second of interval were collected and these data were applied in MATLAB to design algorithm. Also, our group have designed condition of temperature and duration time to apply in both the P-mPCR and the conventional PCR. Both types of PCR performed the same condition of PCR cycle and the outcome was analyzed to investigate the utility of P-mPCR.
Results : For a precise P-mPCR cycle control, our group have investigated the operating preparation time using time-voltage graph, and obtained temperature function using a voltage-temperature graph. We controlled the required voltage of the temperature by investigating temperature function. For utility testing, both conventional and P-mPCR were performed and showed identical data, as a result, we have identified the utility of own invented P-mPCR device.
Conclusions: Our own invented P-mPCR device and a sample channel proved the excellence of PCR and temperature control performance and sample channel used in P-mPCR showed advantage of the mass production according to low cost and disposable use as a bio-chip. After further studies about combination of DNA sample preparation stages and more upgraded chip and device, our group expect this device would be meaningful in gene analysis system market.
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