Biosugar의 효율적인 활용을 위한 furfural 저해 극복 및 잔류효소 활용 연구 Enhancement of Furfural Resistance and Use of Residual Enzyme for Efficient Utilization of Biosugar원문보기
본 연구는 목질계바이오매스로부터 바이오슈가를 생산하는 전 처리 과정에서 발생하는 furfural 저해현상과 당화과정에서 남아있는 당화효소를 효율적으로 활용하기 위한 내용이다. 부산물은 유기산부터 퓨란 화합물, 페놀 화합물까지 다양하게 생성되며 바이오슈가에 남아 미생물의 생장을 방해한다. 이중 furfural이 대표적인 독성물질로 알려져 있으며, 본 연구에서는 PHB생산 유전자를 넣은 Polyhydroxybutyrate synthetic ...
본 연구는 목질계바이오매스로부터 바이오슈가를 생산하는 전 처리 과정에서 발생하는 furfural 저해현상과 당화과정에서 남아있는 당화효소를 효율적으로 활용하기 위한 내용이다. 부산물은 유기산부터 퓨란 화합물, 페놀 화합물까지 다양하게 생성되며 바이오슈가에 남아 미생물의 생장을 방해한다. 이중 furfural이 대표적인 독성물질로 알려져 있으며, 본 연구에서는 PHB생산 유전자를 넣은 Polyhydroxybutyrate synthetic strain (pLW487)를 사용하여 furfuryl alcohol로 빠른 전환과 glucose의 빠른 소모, PHB생산을 통해 furfural 저해 현상을 극복 할 수 있었다. 당화 과정에서 사용되는 효소가 과정이 끝나고 바이오슈가에 남아 생장을 방해하는 원인이 된다. 이를 해결하기 위해 Bacillus subtilis의 protease를 활용하여 남은 효소를 질소원과 아미노산으로 바꾸어 생장에 이용하였다. 이를 효과적으로 활용하기 위해 Bacillus subtilis 재조합 균주를 사용하여 바이오슈가를 기질로 isobutanol 생성 까지 확인하였다. 이를 통해 바이오슈가 생성 과정을 줄여 비용을 절감하고 바이오슈가의 기질로써의 가능성을 보았다. 선행연구에 이어 바이오슈가의 부산물을 효과적으로 생산에 활용하는 방법의 연구를 통해 생산물의 순도를 높이고 양을 증가시키는 생산방법의 개량 및 연구가 지속적으로 이루어져야 할 것이다.
본 연구는 목질계바이오매스로부터 바이오슈가를 생산하는 전 처리 과정에서 발생하는 furfural 저해현상과 당화과정에서 남아있는 당화효소를 효율적으로 활용하기 위한 내용이다. 부산물은 유기산부터 퓨란 화합물, 페놀 화합물까지 다양하게 생성되며 바이오슈가에 남아 미생물의 생장을 방해한다. 이중 furfural이 대표적인 독성물질로 알려져 있으며, 본 연구에서는 PHB생산 유전자를 넣은 Polyhydroxybutyrate synthetic strain (pLW487)를 사용하여 furfuryl alcohol로 빠른 전환과 glucose의 빠른 소모, PHB생산을 통해 furfural 저해 현상을 극복 할 수 있었다. 당화 과정에서 사용되는 효소가 과정이 끝나고 바이오슈가에 남아 생장을 방해하는 원인이 된다. 이를 해결하기 위해 Bacillus subtilis의 protease를 활용하여 남은 효소를 질소원과 아미노산으로 바꾸어 생장에 이용하였다. 이를 효과적으로 활용하기 위해 Bacillus subtilis 재조합 균주를 사용하여 바이오슈가를 기질로 isobutanol 생성 까지 확인하였다. 이를 통해 바이오슈가 생성 과정을 줄여 비용을 절감하고 바이오슈가의 기질로써의 가능성을 보았다. 선행연구에 이어 바이오슈가의 부산물을 효과적으로 생산에 활용하는 방법의 연구를 통해 생산물의 순도를 높이고 양을 증가시키는 생산방법의 개량 및 연구가 지속적으로 이루어져야 할 것이다.
Biosugar from lignocellulosic biomass has advantages over grabbing simultaneously economic and environmental aspects by utilizing waste lumber. However, the chemical process for biosugar takes a long period of time and is not cost-effective so that it is not that much popularized nowadays. Even so t...
Biosugar from lignocellulosic biomass has advantages over grabbing simultaneously economic and environmental aspects by utilizing waste lumber. However, the chemical process for biosugar takes a long period of time and is not cost-effective so that it is not that much popularized nowadays. Even so there are many efforts by researchers to overcome the problems and the market size of biosugar is gradually increasing. The common problem occurs by treatment of lignocellulosic biomass is the by-products produced such as from weak organic acid like acetate to furfural, vanillin etc. These by-products are still contained in biosugar so that they have deleterious effect on cellular growth and biofermentation. Furfural is a representative toxic molecule of which active reseraches are ongoing about tolerance genes toward it. Conversion of furfural into furfuryl alcohol is a common strategy to minimize the toxic effect of furfural. Polyhydroxybutyrate (PHB) accumulating E. coli (pLW487) converts furfural into furfuryl alcohol rapidly so that it has different aspect when compared to the wild type E. coli by reducing the time of lag phase and increasing the simultaneous uptake of glucose and furfural. In addition to the increased cell growth, there is an increased concentration of PHB in furfural added samples from 40% to 60% when compared to culture condition without furfural. Thus we have shown that furfural has a positive effect on increasing the total amount of PHB in E. coli. Other than by-products, enzyme used for the treatment of lignocelluosic biomass, still exist in the biosugar composition so that it inhibits the cell growth as a nirtogen source. Filtration is a way to avoid the problem by enzymes but it is not cost effective. Bacillus subtilis a strain that can utilize proteins by converting it into amino acids. By this way, experiments has been done utilizing biosugar and left over enzymes using Bacillus subtilis as a strain, it has been shown that Bacillus subtilis has better cell growth over other strains. Further utilize biosugar effectively, we used engineered Bacillus subtilis which can produce isobutanol from biosugar as a substrate and identified isobutanol production from the strain. From now on there should be more researches on increasing the amount of PHB and isobutanol and ways to utilize biosugar effectively other than removing the inhibitory factors.
Biosugar from lignocellulosic biomass has advantages over grabbing simultaneously economic and environmental aspects by utilizing waste lumber. However, the chemical process for biosugar takes a long period of time and is not cost-effective so that it is not that much popularized nowadays. Even so there are many efforts by researchers to overcome the problems and the market size of biosugar is gradually increasing. The common problem occurs by treatment of lignocellulosic biomass is the by-products produced such as from weak organic acid like acetate to furfural, vanillin etc. These by-products are still contained in biosugar so that they have deleterious effect on cellular growth and biofermentation. Furfural is a representative toxic molecule of which active reseraches are ongoing about tolerance genes toward it. Conversion of furfural into furfuryl alcohol is a common strategy to minimize the toxic effect of furfural. Polyhydroxybutyrate (PHB) accumulating E. coli (pLW487) converts furfural into furfuryl alcohol rapidly so that it has different aspect when compared to the wild type E. coli by reducing the time of lag phase and increasing the simultaneous uptake of glucose and furfural. In addition to the increased cell growth, there is an increased concentration of PHB in furfural added samples from 40% to 60% when compared to culture condition without furfural. Thus we have shown that furfural has a positive effect on increasing the total amount of PHB in E. coli. Other than by-products, enzyme used for the treatment of lignocelluosic biomass, still exist in the biosugar composition so that it inhibits the cell growth as a nirtogen source. Filtration is a way to avoid the problem by enzymes but it is not cost effective. Bacillus subtilis a strain that can utilize proteins by converting it into amino acids. By this way, experiments has been done utilizing biosugar and left over enzymes using Bacillus subtilis as a strain, it has been shown that Bacillus subtilis has better cell growth over other strains. Further utilize biosugar effectively, we used engineered Bacillus subtilis which can produce isobutanol from biosugar as a substrate and identified isobutanol production from the strain. From now on there should be more researches on increasing the amount of PHB and isobutanol and ways to utilize biosugar effectively other than removing the inhibitory factors.
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