비교 유전체학 및 전사체학을 이용한 병원성 Burkholderia의 식물체 내 특이적 생리활성 전략들에 대한 연구 Studies of In Planta Physiological Strategies of Pathogenic Burkholderia Using Comparative Genomics and Transcriptomics원문보기
Some Burkholderia species cause serious plant diseases such as bacterial panicle blight, sheath/grain rot, and bacterial wilt in many countries. Since most studies about pathogenesis of plant pathogenic Burkholderia have focused on a functional validation of only one or two virulence factors, little...
Some Burkholderia species cause serious plant diseases such as bacterial panicle blight, sheath/grain rot, and bacterial wilt in many countries. Since most studies about pathogenesis of plant pathogenic Burkholderia have focused on a functional validation of only one or two virulence factors, little is known about bacterial physiology, adaptation processes, and virulence mechanisms during infection. In an earlier study, I confirmed the differential regulation of toxoflavin production as a major virulence factor among plant pathogenic Burkholderia. B. gladioli KACC11889 possessed all the genes involved in toxoflavin biosynthesis, but lacked the quorum sensing system that functions as an on/off switch for toxoflavin production. These data suggested that plant pathogenic Burkholderia species have evolved to require a range of regulatory systems to reflect the highly complicated environment of host plants. To gain a better understanding of the actual physiological changes in in planta condition, entire in vivo transcriptomes of B. glumae were constructed from infected rice tissues using an RNA sequencing technology. A comparison of in vivo transcriptomes with in vitro transcriptomes identified 2653 differentially expressed genes (DEGs) comprised of 928 up-regulated genes and 1725 down-regulated genes out of the total 6224 genes. In the KEGG enrichment of the DEGs, most of the genes involved in flagella assembly, bacterial chemotaxis, and two-component systems were very highly enriched. Also, higher up-regulated metabolic pathways were related to ascorbate and trehalose metabolisms and sugar transporters including L-arabinose and D-xylose. In conclusion, it was revealed that B. glumae requires a genome-wide transcriptional regulation in response to in planta condition and for successful pathogenesis. Furthermore, to determine global trends conserved across plant pathogens, a comparative analysis of in planta transcriptomes was performed among major plant pathogenic bacteria, including B. glumae, Ralstonia solanacearum and Xanthomonas oryzae pv. oryzae. By overlaying the in planta transcriptomes on the pan-genomic map of three bacteria, I found in planta-dependent transcriptional patterns in nearly 150 common DEGs. These transcriptional patterns were correlated with changes in signal transduction and collapse of intracellular systems. Through the mutagenesis experiment, it was demonstrated that co-up-regulated DEGs in all three pathogenic bacteria were associated with acquisition of limiting nutrients and degradation of an antibacterial plant metabolite. Comparative analysis of in planta transcriptomes of three different pathogenic bacteria enabled delineation of key genes required for the survival and virulence of each pathogenic bacterium. This approach may be efficiently applied to the identification of common virulence mechanisms during diverse plant-pathogen interactions.
Some Burkholderia species cause serious plant diseases such as bacterial panicle blight, sheath/grain rot, and bacterial wilt in many countries. Since most studies about pathogenesis of plant pathogenic Burkholderia have focused on a functional validation of only one or two virulence factors, little is known about bacterial physiology, adaptation processes, and virulence mechanisms during infection. In an earlier study, I confirmed the differential regulation of toxoflavin production as a major virulence factor among plant pathogenic Burkholderia. B. gladioli KACC11889 possessed all the genes involved in toxoflavin biosynthesis, but lacked the quorum sensing system that functions as an on/off switch for toxoflavin production. These data suggested that plant pathogenic Burkholderia species have evolved to require a range of regulatory systems to reflect the highly complicated environment of host plants. To gain a better understanding of the actual physiological changes in in planta condition, entire in vivo transcriptomes of B. glumae were constructed from infected rice tissues using an RNA sequencing technology. A comparison of in vivo transcriptomes with in vitro transcriptomes identified 2653 differentially expressed genes (DEGs) comprised of 928 up-regulated genes and 1725 down-regulated genes out of the total 6224 genes. In the KEGG enrichment of the DEGs, most of the genes involved in flagella assembly, bacterial chemotaxis, and two-component systems were very highly enriched. Also, higher up-regulated metabolic pathways were related to ascorbate and trehalose metabolisms and sugar transporters including L-arabinose and D-xylose. In conclusion, it was revealed that B. glumae requires a genome-wide transcriptional regulation in response to in planta condition and for successful pathogenesis. Furthermore, to determine global trends conserved across plant pathogens, a comparative analysis of in planta transcriptomes was performed among major plant pathogenic bacteria, including B. glumae, Ralstonia solanacearum and Xanthomonas oryzae pv. oryzae. By overlaying the in planta transcriptomes on the pan-genomic map of three bacteria, I found in planta-dependent transcriptional patterns in nearly 150 common DEGs. These transcriptional patterns were correlated with changes in signal transduction and collapse of intracellular systems. Through the mutagenesis experiment, it was demonstrated that co-up-regulated DEGs in all three pathogenic bacteria were associated with acquisition of limiting nutrients and degradation of an antibacterial plant metabolite. Comparative analysis of in planta transcriptomes of three different pathogenic bacteria enabled delineation of key genes required for the survival and virulence of each pathogenic bacterium. This approach may be efficiently applied to the identification of common virulence mechanisms during diverse plant-pathogen interactions.
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