본 논문은 생강과의 고량강(Alpinia officinarum)으로부터 마우스 대식세포에서 LPS의 의해 유도된 일산화질소(nitric oxide) 생성 저해효과 성분을 규명하고자 하였으며, 고량강 MeOH 추출물의 CH2Cl2층에 대하여 column chromatography 와 preparative-HPLC를 반복 실시하여 총 12종의 화합물을 순수 분리하였음. 순수 분리된 성분에 대하여 물리 화학적 성상과 기존 분리 화합물과의 비교 및 기기분석 데이터를 이용하여 구조를 규명하였고, ...
본 논문은 생강과의 고량강(Alpinia officinarum)으로부터 마우스 대식세포에서 LPS의 의해 유도된 일산화질소(nitric oxide) 생성 저해효과 성분을 규명하고자 하였으며, 고량강 MeOH 추출물의 CH2Cl2층에 대하여 column chromatography 와 preparative-HPLC를 반복 실시하여 총 12종의 화합물을 순수 분리하였음. 순수 분리된 성분에 대하여 물리 화학적 성상과 기존 분리 화합물과의 비교 및 기기분석 데이터를 이용하여 구조를 규명하였고, nitric oxide 생성 저해효과를 측정하였음. 분리된 성분들에 대한 NO 생성 저해 활성을 측정환 결과 일부 diarylheptanoid 화합물에서 11.0~97.0 M 범위에서 50% 저해농도를 보였음. 각종 염증 및 자가 면역질환 등에 관여하는 물질로 알려진 NO 생성 저해효과를 보인 고량강은 향후 NO 관련질환 치료제 개발에 연구할 가치가 있는 자원식물로 사료됨.
본 논문은 생강과의 고량강(Alpinia officinarum)으로부터 마우스 대식세포에서 LPS의 의해 유도된 일산화질소(nitric oxide) 생성 저해효과 성분을 규명하고자 하였으며, 고량강 MeOH 추출물의 CH2Cl2층에 대하여 column chromatography 와 preparative-HPLC를 반복 실시하여 총 12종의 화합물을 순수 분리하였음. 순수 분리된 성분에 대하여 물리 화학적 성상과 기존 분리 화합물과의 비교 및 기기분석 데이터를 이용하여 구조를 규명하였고, nitric oxide 생성 저해효과를 측정하였음. 분리된 성분들에 대한 NO 생성 저해 활성을 측정환 결과 일부 diarylheptanoid 화합물에서 11.0~97.0 M 범위에서 50% 저해농도를 보였음. 각종 염증 및 자가 면역질환 등에 관여하는 물질로 알려진 NO 생성 저해효과를 보인 고량강은 향후 NO 관련질환 치료제 개발에 연구할 가치가 있는 자원식물로 사료됨.
The rhizome of Alpinia officinarium (Zingiberaceae) is a well-known Korean traditional medicine, and has been used for indigestion, vomiting and abdominal pain. The purpose of this investigation was to isolate bioactive compounds from A. officinarum. The MeOH extract of A. officinarum was fract...
The rhizome of Alpinia officinarium (Zingiberaceae) is a well-known Korean traditional medicine, and has been used for indigestion, vomiting and abdominal pain. The purpose of this investigation was to isolate bioactive compounds from A. officinarum. The MeOH extract of A. officinarum was fractionated with n-hexane, CH2Cl2, EtOAc and n-butanol. Of these, CH2Cl2 fraction was separated by various chromatographic techniques such as silica gel, sephadex LH-20, RP-C18 MPLC, and preparative HPLC. The structures of isolated compounds were determined on the basis of spectroscopic data including 1H-NMR and 13C-NMR. As a result, twelve known compounds, 1,7-diphenyl-5-hydroxy-3-heptanone (1), 5-methoxy-1,7-diphenyl-3-heptanone (2), 7-(4-hydroxy-3-methoxy phenyl)-1-phenyl-4-hepten-3-one (3), 5-hydroxy-7-(4-hydroxy-3-methoxy phenyl)-1-phenyl-3-heptanone (4), 1,7-diphenyl-4-hepten-3-one (5), 5-hydroxy-1-(4-hydroxyphenyl)-7-phenyl-3-heptanone, (6), 3,5-Heptanediol,1-(41hydroxyphenyl)-7-phenyl (7), 3,5-heptanediol-1-(4-hydroxy phenyl)-7-phenyl (8), 3,5-heptanediol-1-(4-hydroxy-3-methoxyphenyl)-7-phenyl (9), 3,5-heptanediol-1,7-bis(4-hydroxy-3-methoxyphenyl) (10), kaempferide (11), and galangin (12) were isolated and identified. All compounds were evaluated for their inhibitory effects on NO production against LPS-induced RAW 264.7 macrophage. As a result, diarylheptanoids 3, 5, 7, and 10 showed inhibitory effect on NO production with IC50 values of 25.1 - 78.0 M. Further studies are needed to elucidate the mechanism of action including their target molecules for the inhibition of nitric oxide production.
The rhizome of Alpinia officinarium (Zingiberaceae) is a well-known Korean traditional medicine, and has been used for indigestion, vomiting and abdominal pain. The purpose of this investigation was to isolate bioactive compounds from A. officinarum. The MeOH extract of A. officinarum was fractionated with n-hexane, CH2Cl2, EtOAc and n-butanol. Of these, CH2Cl2 fraction was separated by various chromatographic techniques such as silica gel, sephadex LH-20, RP-C18 MPLC, and preparative HPLC. The structures of isolated compounds were determined on the basis of spectroscopic data including 1H-NMR and 13C-NMR. As a result, twelve known compounds, 1,7-diphenyl-5-hydroxy-3-heptanone (1), 5-methoxy-1,7-diphenyl-3-heptanone (2), 7-(4-hydroxy-3-methoxy phenyl)-1-phenyl-4-hepten-3-one (3), 5-hydroxy-7-(4-hydroxy-3-methoxy phenyl)-1-phenyl-3-heptanone (4), 1,7-diphenyl-4-hepten-3-one (5), 5-hydroxy-1-(4-hydroxyphenyl)-7-phenyl-3-heptanone, (6), 3,5-Heptanediol,1-(41hydroxyphenyl)-7-phenyl (7), 3,5-heptanediol-1-(4-hydroxy phenyl)-7-phenyl (8), 3,5-heptanediol-1-(4-hydroxy-3-methoxyphenyl)-7-phenyl (9), 3,5-heptanediol-1,7-bis(4-hydroxy-3-methoxyphenyl) (10), kaempferide (11), and galangin (12) were isolated and identified. All compounds were evaluated for their inhibitory effects on NO production against LPS-induced RAW 264.7 macrophage. As a result, diarylheptanoids 3, 5, 7, and 10 showed inhibitory effect on NO production with IC50 values of 25.1 - 78.0 M. Further studies are needed to elucidate the mechanism of action including their target molecules for the inhibition of nitric oxide production.
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