본 연구는 한방과 민간에서 약용식물로 알려진 접골목 잎에 대해 천연 화장품 소재로서의 활용 가능성을 알아보고자 실시하였다. 이를 위해 건조된 접골목 잎을 에탄올추출법을 통해 유효성분을 추출하여, 접골목 잎 에탄올추출물의 항산화 활성, 세포독성, 항염 효능을 측정하였다. 실험 결과는 다음과 같다. 첫째, 접골목 잎 에탄올추출물의 항산화 활성을 측정하기 위해 ...
본 연구는 한방과 민간에서 약용식물로 알려진 접골목 잎에 대해 천연 화장품 소재로서의 활용 가능성을 알아보고자 실시하였다. 이를 위해 건조된 접골목 잎을 에탄올추출법을 통해 유효성분을 추출하여, 접골목 잎 에탄올추출물의 항산화 활성, 세포독성, 항염 효능을 측정하였다. 실험 결과는 다음과 같다. 첫째, 접골목 잎 에탄올추출물의 항산화 활성을 측정하기 위해 DPPH, ABTS, FRAP, TPC 측정을 실시하였다. DPPH 라디컬 소거능 측정 결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, 각각 81.27%, 84.80%, 89.90%, 90.58% 농도 의존적으로 높은 DPPH 라디컬 소거능이 확인되었다. ABTS 라디컬 소거능 측정 결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, 각각 55.86%, 88.20%, 88.71%, 91.47%로 나타나 농도 의존적으로 소거능이 증가하는 것으로 나타났다. FRAP 철이온 환원력 측정결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml 농도로 처리 시, 각각 95.52mM, 169.99mM, 196.20mM, 199.52mM로 농도 의존적으로 철이온 환원력이 증가되는 것을 확인할 수 있었다. 총폴리페놀함량(TPC)은 53.82 ugGAE/ml으로 측정되어 접골목 잎 추출물의 항산화 효능이 있는 것으로 확인되었다. 둘째, 접골목 잎 에탄올추출물의 세포독성에 대한 안전성을 평가하기 위해 RAW264.7 cell을 이용하여 MTT assay를 실시한 결과, LPS(100 ng/ml) 처리 후 세포생존율은 112.57%로 나타났다. 각 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml,의 농도로 처리 시, 각각 102.46%, 97.79%, 89.31%. 25.36%, 생존율을 나타내 1.25 mg/ml 이하 농도에서 세포에 대한 안전성을 확인 할 수 있다. 셋째, LPS로 자극한 RAW264.7 cell에서 접골목 잎 에탄올추출물의 항염 효능을 알아보기 위해 NO 생성량, TNF-α 분비량, iNOS mRNA발현수준, TNF-α mRNA의 발현수준을 측정하였다. NO 생성 저해능 측정 결과, RAW264.7 cell에서 LPS를 인하여 NO 생성이 8.68 μM수준으로 현저하게 증가하였다. 반면 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, NO 생성 농도는 각각 6.5μM, 5.0 μM, 3.7 μM, 1.9 μM로 나타나 농도 의존적으로 NO 생성량이 억제되는 것을 확인 할 수 있었다. NO의 합성효소인 iNOS의 유전자 mRNA의 발현수준을 조사한 결과, 안정한 대식세포를 LPS로 처리하여 추출물을 농도별로 각각 처리 시, 농도 의존적으로 iNOS의 mRNA 발현이 저해된 것을 확인할 수 있었다. 전염증성 사이토카인 TNF-α 분비 및, TNF-α의 mRNA 발현수준 실험 결과, LPS가 TNF-α, TNF-α mRNA의 분비량과 발현수준을 현저하게 증가시켰으나, 추출물을 농도별로 처리 시 LPS 대조군에 비해 농도 의존적으로 분비량과 발현수준이 억제된 것을 확인 할 수 있었다. 이상의 연구 결과를 통해, 접골목 잎 에탄올추출물은 세포에 대한 안전성이 확인되었고, 우수한 항산화 활성과 항염 효능이 확인되었다. 이에 접골목 잎은 천연화장품 소재로서 피부 노화지연과 염증완화에 대해 높은 활용가치가 있는 것으로 판단된다.
본 연구는 한방과 민간에서 약용식물로 알려진 접골목 잎에 대해 천연 화장품 소재로서의 활용 가능성을 알아보고자 실시하였다. 이를 위해 건조된 접골목 잎을 에탄올추출법을 통해 유효성분을 추출하여, 접골목 잎 에탄올추출물의 항산화 활성, 세포독성, 항염 효능을 측정하였다. 실험 결과는 다음과 같다. 첫째, 접골목 잎 에탄올추출물의 항산화 활성을 측정하기 위해 DPPH, ABTS, FRAP, TPC 측정을 실시하였다. DPPH 라디컬 소거능 측정 결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, 각각 81.27%, 84.80%, 89.90%, 90.58% 농도 의존적으로 높은 DPPH 라디컬 소거능이 확인되었다. ABTS 라디컬 소거능 측정 결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, 각각 55.86%, 88.20%, 88.71%, 91.47%로 나타나 농도 의존적으로 소거능이 증가하는 것으로 나타났다. FRAP 철이온 환원력 측정결과, 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml 농도로 처리 시, 각각 95.52mM, 169.99mM, 196.20mM, 199.52mM로 농도 의존적으로 철이온 환원력이 증가되는 것을 확인할 수 있었다. 총폴리페놀함량(TPC)은 53.82 ugGAE/ml으로 측정되어 접골목 잎 추출물의 항산화 효능이 있는 것으로 확인되었다. 둘째, 접골목 잎 에탄올추출물의 세포독성에 대한 안전성을 평가하기 위해 RAW264.7 cell을 이용하여 MTT assay를 실시한 결과, LPS(100 ng/ml) 처리 후 세포생존율은 112.57%로 나타났다. 각 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml,의 농도로 처리 시, 각각 102.46%, 97.79%, 89.31%. 25.36%, 생존율을 나타내 1.25 mg/ml 이하 농도에서 세포에 대한 안전성을 확인 할 수 있다. 셋째, LPS로 자극한 RAW264.7 cell에서 접골목 잎 에탄올추출물의 항염 효능을 알아보기 위해 NO 생성량, TNF-α 분비량, iNOS mRNA발현수준, TNF-α mRNA의 발현수준을 측정하였다. NO 생성 저해능 측정 결과, RAW264.7 cell에서 LPS를 인하여 NO 생성이 8.68 μM수준으로 현저하게 증가하였다. 반면 추출물을 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml의 농도로 처리 시, NO 생성 농도는 각각 6.5μM, 5.0 μM, 3.7 μM, 1.9 μM로 나타나 농도 의존적으로 NO 생성량이 억제되는 것을 확인 할 수 있었다. NO의 합성효소인 iNOS의 유전자 mRNA의 발현수준을 조사한 결과, 안정한 대식세포를 LPS로 처리하여 추출물을 농도별로 각각 처리 시, 농도 의존적으로 iNOS의 mRNA 발현이 저해된 것을 확인할 수 있었다. 전염증성 사이토카인 TNF-α 분비 및, TNF-α의 mRNA 발현수준 실험 결과, LPS가 TNF-α, TNF-α mRNA의 분비량과 발현수준을 현저하게 증가시켰으나, 추출물을 농도별로 처리 시 LPS 대조군에 비해 농도 의존적으로 분비량과 발현수준이 억제된 것을 확인 할 수 있었다. 이상의 연구 결과를 통해, 접골목 잎 에탄올추출물은 세포에 대한 안전성이 확인되었고, 우수한 항산화 활성과 항염 효능이 확인되었다. 이에 접골목 잎은 천연화장품 소재로서 피부 노화지연과 염증완화에 대해 높은 활용가치가 있는 것으로 판단된다.
The purpose of this study was to investigate the possibility of using sambucus williamsii leaf as natural cosmetics materials, which were used as medicinal plants in Korean civil tradition. The effective components of the dried sambucus williamsii leaves were extracted by ethanol extraction method. ...
The purpose of this study was to investigate the possibility of using sambucus williamsii leaf as natural cosmetics materials, which were used as medicinal plants in Korean civil tradition. The effective components of the dried sambucus williamsii leaves were extracted by ethanol extraction method. The antioxidant activity, cytotoxicity, and anti-inflammatory of the sambucus williamsii leaves extracts were analyzed and estimated. The results are as follows. Firstly, the DPPH, ABTS, FRAP, and TPC methods were performed to measure the antioxidant activity of ethanol extracts of sambucus williamsii leaves. When the extracts were treated at the concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, the concentration DPPH radical scavenging activity increased from 81.27%, 84.80%, 89.90% to 90.58%, respectively which was confirmed. For ABTS radical scavenging activity, the concentration was 55.86%, 88.20%, 88.71%, and 91.47% which had been shown to increased when extracts were treated at concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, and 2.5 mg/ml. As a result of the measurement of FRAP iron ion reducing ability, they were 95.52mM, 169.99mM, 196.20mM, and 199.52mM, respectively. The increase of reducing power was confirmed when extracts were treated at the concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, respectively. The total polyphenol content (TPC) was 53.82 ugGAE/ml, The antioxidant ability of sambucus williamsii leaves extracts had been identified. Secondly, MTT assay was performed in RAW264.7 cells to evaluate the cytotoxicity of the ethanol sambucus williamsii leaves extract. After the treatment with LPS (100 ng / ml), the result of the cell survival rate was 112.57%. When the extract was treated at the concentration of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml respectively, the survival rate was 102.46%, 97.79%, 89.31% and 25.36%, respectively. Thus, when the concentration of the extract was 1.25 mg/ml or at a less level, The safety of cell was found to be more significant. Thirdly, production content of NO, secretion of TNF-α, the iNOS mRNA manifestation level, and the TNF-α mRNA manifestation levels were measured to determine the anti-inflammatory effects of sambucus williamsii leaves extract in LPS-stimulated RAW264.7 cells. The results of NO generation inhibitory measurements showed that NO generation was significantly increased to 8.68 μM level due to LPS in RAW264.7 cells. After treatment with the extracts at the concentration of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, NO generation concentrations were reduced from 6,5 μM, 5.0 μM, 3.7 μM to 1.9 μM. Through this result, the inhibition ability was confirmed. The result of the manifestation level of the gene mRNA of enzyme iNOS, the synthetase of NO showed that the manifestation of iNOS mRNA was inhibited by each concentration of extract when the stable macrophage was treated with LPS. The results of the manifestation level of inflammatory cytokines TNF-α and TNF-α mRNA showed that the levels of secretion and expression of TNF-α and TNF-α mRNA increased significantly, when the extract was treated by different concentrations. It was found that the secretion and manifestation level was inhibited, comparing with LPS control group. Through the result of this study, the ethanol extract of sambucus williamsii leaves was confirmed to be safe for cells, and excellent antioxidant activity and anti-inflammatory efficacy were confirmed as a natural cosmetics material, These leaves would be considered to have high utilization value for skin aging delay and inflammation alleviation.
The purpose of this study was to investigate the possibility of using sambucus williamsii leaf as natural cosmetics materials, which were used as medicinal plants in Korean civil tradition. The effective components of the dried sambucus williamsii leaves were extracted by ethanol extraction method. The antioxidant activity, cytotoxicity, and anti-inflammatory of the sambucus williamsii leaves extracts were analyzed and estimated. The results are as follows. Firstly, the DPPH, ABTS, FRAP, and TPC methods were performed to measure the antioxidant activity of ethanol extracts of sambucus williamsii leaves. When the extracts were treated at the concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, the concentration DPPH radical scavenging activity increased from 81.27%, 84.80%, 89.90% to 90.58%, respectively which was confirmed. For ABTS radical scavenging activity, the concentration was 55.86%, 88.20%, 88.71%, and 91.47% which had been shown to increased when extracts were treated at concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, and 2.5 mg/ml. As a result of the measurement of FRAP iron ion reducing ability, they were 95.52mM, 169.99mM, 196.20mM, and 199.52mM, respectively. The increase of reducing power was confirmed when extracts were treated at the concentrations of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, respectively. The total polyphenol content (TPC) was 53.82 ugGAE/ml, The antioxidant ability of sambucus williamsii leaves extracts had been identified. Secondly, MTT assay was performed in RAW264.7 cells to evaluate the cytotoxicity of the ethanol sambucus williamsii leaves extract. After the treatment with LPS (100 ng / ml), the result of the cell survival rate was 112.57%. When the extract was treated at the concentration of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 2.5 mg/ml respectively, the survival rate was 102.46%, 97.79%, 89.31% and 25.36%, respectively. Thus, when the concentration of the extract was 1.25 mg/ml or at a less level, The safety of cell was found to be more significant. Thirdly, production content of NO, secretion of TNF-α, the iNOS mRNA manifestation level, and the TNF-α mRNA manifestation levels were measured to determine the anti-inflammatory effects of sambucus williamsii leaves extract in LPS-stimulated RAW264.7 cells. The results of NO generation inhibitory measurements showed that NO generation was significantly increased to 8.68 μM level due to LPS in RAW264.7 cells. After treatment with the extracts at the concentration of 0.3125 mg/ml, 0.625 mg/ml, 1.25 mg/ml and 2.5 mg/ml, NO generation concentrations were reduced from 6,5 μM, 5.0 μM, 3.7 μM to 1.9 μM. Through this result, the inhibition ability was confirmed. The result of the manifestation level of the gene mRNA of enzyme iNOS, the synthetase of NO showed that the manifestation of iNOS mRNA was inhibited by each concentration of extract when the stable macrophage was treated with LPS. The results of the manifestation level of inflammatory cytokines TNF-α and TNF-α mRNA showed that the levels of secretion and expression of TNF-α and TNF-α mRNA increased significantly, when the extract was treated by different concentrations. It was found that the secretion and manifestation level was inhibited, comparing with LPS control group. Through the result of this study, the ethanol extract of sambucus williamsii leaves was confirmed to be safe for cells, and excellent antioxidant activity and anti-inflammatory efficacy were confirmed as a natural cosmetics material, These leaves would be considered to have high utilization value for skin aging delay and inflammation alleviation.
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