The purpose of this study was to investigate activities as functional cosmetic materials, focusing on Rhododendron brachycarpum and Rhododendron fortunei.
The electron donating ability of 70% ethanol extract from Rhododendron brachycarpum and Rhododendron fortunei extracts shown respectively 85....
The purpose of this study was to investigate activities as functional cosmetic materials, focusing on Rhododendron brachycarpum and Rhododendron fortunei.
The electron donating ability of 70% ethanol extract from Rhododendron brachycarpum and Rhododendron fortunei extracts shown respectively 85.8%, 86.2% at 500 μg/ml concentration. The ABTS+ radical scavenging ability of both extracts showed approximately 91% at 500 μg/ml concentration. The tyrosinase inhibitory effect which is related to skin-whitening, was respectively 32.6%, 39.3% at the concentration of 1,000 μg/ml. The elastase inhibitory effect which is related to skin anti-wrinkles, was 30%, 36.2% at 1,000 μg/ml concentration. Moreover, collagenase inhibitory activity shown respectively 77.7%, 80.2% at 1,000 μg/ml concentration, that showed excellent inhibitory activity.
For a cell viability test, measured on keratinocyte cell (HaCaT) and fibroblast cell (CCD-986sk) by 70% ethanol extract of Rhododendron brachycarpum and Rhododendron fortunei. Results shown respectively 96.1%, 92.5% with cell viability at 10 μg/ml concentration in HaCaT. Furthermore, cell viability test showed 100.9%, 98.9% with cell viability at 100 μg/ml concentration in CCD-986sk. In addition, Rhododendron brachycarpum and Rhododendron fortunei extracts shown effect of cell protection, through ROS erasing activity measurement that preventing photo-aging caused by ultraviolet light, thereby antioxidant activity.
The mRNA expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by real time-PCR at 2, 5, 10 μg/ml concentrations. In consequence, mRNA expression of SOD-1, catalase and GPx-1 were slightly decreased when treated with UVB. However, when UVB with Rhododendron brachycarpum and Rhododendron fortunei extracts were treated at the same time, the expression level there of increased in a concentration-dependent. Therefore, the effect of Rhododendron brachycarpum and Rhododendron fortunei extracts on the gene level of antioxidant enzymes was confirmed.
The protein expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by western blot at 25, 50, 100 μg/ml concentrations and the β-actin used as a positive control. As a result, western blot of 70% ethanol extract from Rhododendron brachycarpum the expression inhibition rate of the MMP-1, MMP-2, MMP-3 protein was decreased by 67.2%, 65.5%, 13.6% at 100 μg/ml concentration. Besides, the results of western blot of ethanol extract from Rhododendron fortunei the expression inhibition rate of the same protein as before was decreased by 89.1%, 85.0%, 62.7% at 100 μg/ml concentration.
The mRNA expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by reverse transcription-PCR at 25, 50, 100 μg/ml concentrations and the GAPDH used as a positive control. According to the results of reverse transcription-PCR of 70% ethanol extract from Rhododendron brachycarpum and Rhododendron fortunei the expression inhibition rate of the MMP-1, MMP-2, MMP-3 mRNA was decreased by 70.1%, 9.1%, 37.9% and 38.2%, 8.3%, 57.3% at 100 μg/ml concentrations. Finally, results of perform the BCOP experiment that Rhododendron brachycarpum and Rhododendron fortunei extracts showed an IVIS value of no stimulation up to a concentration of 1 mg/ml. So, Rhododendron brachycarpum and Rhododendron fortunei extracts showed the antioxidant, anti-wrinkle effects and safety as a funtional cosmetics material.
The purpose of this study was to investigate activities as functional cosmetic materials, focusing on Rhododendron brachycarpum and Rhododendron fortunei.
The electron donating ability of 70% ethanol extract from Rhododendron brachycarpum and Rhododendron fortunei extracts shown respectively 85.8%, 86.2% at 500 μg/ml concentration. The ABTS+ radical scavenging ability of both extracts showed approximately 91% at 500 μg/ml concentration. The tyrosinase inhibitory effect which is related to skin-whitening, was respectively 32.6%, 39.3% at the concentration of 1,000 μg/ml. The elastase inhibitory effect which is related to skin anti-wrinkles, was 30%, 36.2% at 1,000 μg/ml concentration. Moreover, collagenase inhibitory activity shown respectively 77.7%, 80.2% at 1,000 μg/ml concentration, that showed excellent inhibitory activity.
For a cell viability test, measured on keratinocyte cell (HaCaT) and fibroblast cell (CCD-986sk) by 70% ethanol extract of Rhododendron brachycarpum and Rhododendron fortunei. Results shown respectively 96.1%, 92.5% with cell viability at 10 μg/ml concentration in HaCaT. Furthermore, cell viability test showed 100.9%, 98.9% with cell viability at 100 μg/ml concentration in CCD-986sk. In addition, Rhododendron brachycarpum and Rhododendron fortunei extracts shown effect of cell protection, through ROS erasing activity measurement that preventing photo-aging caused by ultraviolet light, thereby antioxidant activity.
The mRNA expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by real time-PCR at 2, 5, 10 μg/ml concentrations. In consequence, mRNA expression of SOD-1, catalase and GPx-1 were slightly decreased when treated with UVB. However, when UVB with Rhododendron brachycarpum and Rhododendron fortunei extracts were treated at the same time, the expression level there of increased in a concentration-dependent. Therefore, the effect of Rhododendron brachycarpum and Rhododendron fortunei extracts on the gene level of antioxidant enzymes was confirmed.
The protein expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by western blot at 25, 50, 100 μg/ml concentrations and the β-actin used as a positive control. As a result, western blot of 70% ethanol extract from Rhododendron brachycarpum the expression inhibition rate of the MMP-1, MMP-2, MMP-3 protein was decreased by 67.2%, 65.5%, 13.6% at 100 μg/ml concentration. Besides, the results of western blot of ethanol extract from Rhododendron fortunei the expression inhibition rate of the same protein as before was decreased by 89.1%, 85.0%, 62.7% at 100 μg/ml concentration.
The mRNA expression inhibitory effect of Rhododendron brachycarpum and Rhododendron fortunei extracts were measured by reverse transcription-PCR at 25, 50, 100 μg/ml concentrations and the GAPDH used as a positive control. According to the results of reverse transcription-PCR of 70% ethanol extract from Rhododendron brachycarpum and Rhododendron fortunei the expression inhibition rate of the MMP-1, MMP-2, MMP-3 mRNA was decreased by 70.1%, 9.1%, 37.9% and 38.2%, 8.3%, 57.3% at 100 μg/ml concentrations. Finally, results of perform the BCOP experiment that Rhododendron brachycarpum and Rhododendron fortunei extracts showed an IVIS value of no stimulation up to a concentration of 1 mg/ml. So, Rhododendron brachycarpum and Rhododendron fortunei extracts showed the antioxidant, anti-wrinkle effects and safety as a funtional cosmetics material.
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