The interest in health, which was considered the exclusive property of the middle-aged and older people, is emerging as a global concern, transcending age and gender, as new infectious diseases such as Corona 19 threaten the health of people around the world due to climate change caused by environme...
The interest in health, which was considered the exclusive property of the middle-aged and older people, is emerging as a global concern, transcending age and gender, as new infectious diseases such as Corona 19 threaten the health of people around the world due to climate change caused by environmental pollution. Accordingly, the functional material and food market that can promote health is steadily growing. In addition, the inner beauty market, which pursues healthy beauty from the inside, is also growing rapidly due to a change in perception to maintain a more youthful and beautiful health along with an increase in the number of self-care people who want to take care of their health.
Collagen, which decreases with age and causes a decrease in skin elasticity, hyaluronic acid related to skin moisture, and ceramide, which is a major lipid component of the stratum corneum of the skin, strengthens the skin barrier and prevents moisture evaporation, have been known as skin health materials. These functional materials are being applied to cosmetics and functional foods for skin health. Recently, as natural materials-derived materials are in the spotlight, fermented natural extracts and probiotics are being researched and developed as major skin-related functional materials. Fermented natural extracts are fermented extracts of herbs, medicinal plants, and seaweeds with beneficial microorganisms such as lactic acid bacteria. In Korea, the industrialization of skin health functional materials has been made based on the research results of natural extracts that help maintain skin health from UV damage or skin moisturize.
Photo-aging refers to damage to the skin caused by ultraviolet rays and oxidative stress, which increases the production of free radicals by decreasing the expression of antioxidant enzymes. It induces changes in the intracellular signal transduction system and increases the activity of matrix metalloproteinases (MMPs), thereby promoting skin photo-aging such as reduction of skin elasticity and generation of wrinkles.
Ultraviolet rays are divided into UVA, UVB, and UVC depending on the wavelength of the ultraviolet rays. Among them, UVB has more than 1,000 times the intensity compared to other wavelengths, so it causes skin erythema, sunburn, etc. when exposed to acute exposure, and causes dryness when exposed to chronic conditions. It is the main cause of photo-aging by causing deep, rough wrinkles and skin cancer.
In this study, the photo-aging inhibitory effects of natural fermented extracts and probiotics derived from Jeju natural resources and breast milk were investigated in human fibroblasts and SKH-1 hairless mice. In addition, a mass production process was developed for the application of inner beauty functional materials.
In Chapter 1, Gelidium amansii and Cirsium japonicum, which have excellent procollagen synthesis ability, were selected and the mixture was fermented with lactic acid bacteria to confirm the effect of preventing photo-aging of the skin. The effect of fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) on UVB-induced photo-aging was evaluated in human dermal fibroblasts (Hs68 cell) and SKH-1 hairless mice. In vitro, FGCM increased type I procollagen levels and suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression more effectively than G. amansii and C. japonicum extract mixture (GCM). In vivo, oral administration of FGCM significantly inhibited UVB-induced the number and total depth of wrinkles in the dorsal skin of mice. FGCM suppressed UVB-induced epidermal thickening, and attenuated UVB-induced MMP-13 expression and MMP-2 and MMP-9 activities in dermal tissue. Furthermore, FGCM increased skin hydration and blocked transepidermal water loss in the dorsal skin of mice compared with the UVB-irradiated group. These data indicate that FGCM exerts potent anti-photo-aging activities by improving wrinkle formation and dryness.
Probiotics, defined as living microorganisms that enter the body and have beneficial health effects when consumed in moderation, recent clinical studies have reported that probiotics may exert other health-promoting effects beyond gut health. In addition to lowering blood cholesterol, preventing tooth decay, alleviating menopausal symptoms, and immune modulation, it has been studied to exert skin potential such as atopic dermatitis, healing burns and scars, skin rejuvenation properties and improving the skin's innate immunity.
In Chapter 2, we evaluated the effect of Lactiplantibacillus plantarum HY7714 against UVB-induced photo-aging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited phosphorylation of Jun N-terminal kinase (JNK), thereby suppressing UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue.
For the industrialization of L. plantarum HY7714 as a probiotic with skin photo-aging inhibition, high-concentration powdering technology and maintaining shelf life stability are essential. Since functional probiotic raw materials has to maintain 1.0 ×1011CFU/g for shelf life stability, this study aimed to produce high-concentration powder of L. plantarum HY7714 and maintain stability. The number of viable cells for each process after optimization was 5.90×109cfu/ml in culture medium and 4.2×1011CFU/g in freeze-dried powder to produce high-concentration probiotic powder. As a result of the stability test, 1×1011CFU/g was maintained until 16 weeks to develop a commercial probiotic with a skin photo-aging inhibitory function. These results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin health ingredients.
Overall, it was confirmed that fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) and Lactiplantibacillus plantarum HY7714 on UVB-induced skin photo-aging inhibitory effects and could expand industrial applicability as skin health ingredients that can be safely consumed as food. Due to this, its applicability is expected to be expanded not only in the recently growing inner beauty food market, but also in the cosmetics and personal care markets.
Chronic ultraviolet (UV) light causes skin photo-aging, characterized by fine and coarse wrinkle formation and dryness. Among the native plants of Jeju-island, Gelidium amansii and Cirsium japonicum, which have excellent procollagen synthesis ability, were selected and the mixture was fermented with lactic acid bacteria to confirm the effect of preventing photo-aging of the skin. The effect of fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) on UVB-induced photo-aging was evaluated in human dermal fibroblasts (Hs68 cell) and SKH-1 hairless mice. In vitro, FGCM increased type I procollagen levels and suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression more effectively than G. amansii and C. japonicum extract mixture (GCM). In vivo, oral administration of FGCM significantly inhibited UVB-induced the number and total depth of wrinkles in the dorsal skin of mice. FGCM suppressed UVB-induced epidermal thickening, and attenuated UVB-induced MMP-13 expression and MMP-2 and MMP-9 activities in dermal tissue. Furthermore, FGCM increased skin hydration and blocked transepidermal water loss in the dorsal skin of mice compared with the UVB-irradiated group. These data indicate that FGCM exerts potent anti-photo-aging activities by improving wrinkle formation and dryness.
Keywords: Anti-wrinkle, Cirsium japonicum, Gelidium amansii, Skin hydration, Dryness,
Fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM), Photo-aging
Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photo-aging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photo-aging. In this study, we evaluated the effect of Lactiplantibacillus plantarum HY7714 against UVB-induced photo-aging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited phosphorylation of Jun N-terminal kinase (JNK), thereby suppressing UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue.
For the industrialization of L. plantarum HY7714 as a probiotic with skin photo-aging inhibition, high-concentration powdering technology and maintaining shelf life stability are essential. Since functional probiotic raw materials has to maintain 1.0 ×1011 CFU/g for shelf life stability, this study aimed to produce high-concentration powder of L. plantarum HY7714 and maintain stability. The number of viable cells for each process after optimization was 5.90×109 CFU/mL in culture medium and 4.2×1011 CFU/g in freeze-dried powder to produce high-concentration probiotic powder. As a result of the stability test, 1×1011 CFU/g was maintained until 16 weeks to develop a commercial probiotic with a skin photo-aging inhibitory function.
Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin health ingredients.
Key words
Lactiplantibacillus plantarum, photo-aging, probiotics, ultraviolet B (UVB), Skin hydration, Anti-, Industrialization, shelf life stability, Optimization, Skin health ingredients
The interest in health, which was considered the exclusive property of the middle-aged and older people, is emerging as a global concern, transcending age and gender, as new infectious diseases such as Corona 19 threaten the health of people around the world due to climate change caused by environmental pollution. Accordingly, the functional material and food market that can promote health is steadily growing. In addition, the inner beauty market, which pursues healthy beauty from the inside, is also growing rapidly due to a change in perception to maintain a more youthful and beautiful health along with an increase in the number of self-care people who want to take care of their health.
Collagen, which decreases with age and causes a decrease in skin elasticity, hyaluronic acid related to skin moisture, and ceramide, which is a major lipid component of the stratum corneum of the skin, strengthens the skin barrier and prevents moisture evaporation, have been known as skin health materials. These functional materials are being applied to cosmetics and functional foods for skin health. Recently, as natural materials-derived materials are in the spotlight, fermented natural extracts and probiotics are being researched and developed as major skin-related functional materials. Fermented natural extracts are fermented extracts of herbs, medicinal plants, and seaweeds with beneficial microorganisms such as lactic acid bacteria. In Korea, the industrialization of skin health functional materials has been made based on the research results of natural extracts that help maintain skin health from UV damage or skin moisturize.
Photo-aging refers to damage to the skin caused by ultraviolet rays and oxidative stress, which increases the production of free radicals by decreasing the expression of antioxidant enzymes. It induces changes in the intracellular signal transduction system and increases the activity of matrix metalloproteinases (MMPs), thereby promoting skin photo-aging such as reduction of skin elasticity and generation of wrinkles.
Ultraviolet rays are divided into UVA, UVB, and UVC depending on the wavelength of the ultraviolet rays. Among them, UVB has more than 1,000 times the intensity compared to other wavelengths, so it causes skin erythema, sunburn, etc. when exposed to acute exposure, and causes dryness when exposed to chronic conditions. It is the main cause of photo-aging by causing deep, rough wrinkles and skin cancer.
In this study, the photo-aging inhibitory effects of natural fermented extracts and probiotics derived from Jeju natural resources and breast milk were investigated in human fibroblasts and SKH-1 hairless mice. In addition, a mass production process was developed for the application of inner beauty functional materials.
In Chapter 1, Gelidium amansii and Cirsium japonicum, which have excellent procollagen synthesis ability, were selected and the mixture was fermented with lactic acid bacteria to confirm the effect of preventing photo-aging of the skin. The effect of fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) on UVB-induced photo-aging was evaluated in human dermal fibroblasts (Hs68 cell) and SKH-1 hairless mice. In vitro, FGCM increased type I procollagen levels and suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression more effectively than G. amansii and C. japonicum extract mixture (GCM). In vivo, oral administration of FGCM significantly inhibited UVB-induced the number and total depth of wrinkles in the dorsal skin of mice. FGCM suppressed UVB-induced epidermal thickening, and attenuated UVB-induced MMP-13 expression and MMP-2 and MMP-9 activities in dermal tissue. Furthermore, FGCM increased skin hydration and blocked transepidermal water loss in the dorsal skin of mice compared with the UVB-irradiated group. These data indicate that FGCM exerts potent anti-photo-aging activities by improving wrinkle formation and dryness.
Probiotics, defined as living microorganisms that enter the body and have beneficial health effects when consumed in moderation, recent clinical studies have reported that probiotics may exert other health-promoting effects beyond gut health. In addition to lowering blood cholesterol, preventing tooth decay, alleviating menopausal symptoms, and immune modulation, it has been studied to exert skin potential such as atopic dermatitis, healing burns and scars, skin rejuvenation properties and improving the skin's innate immunity.
In Chapter 2, we evaluated the effect of Lactiplantibacillus plantarum HY7714 against UVB-induced photo-aging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited phosphorylation of Jun N-terminal kinase (JNK), thereby suppressing UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue.
For the industrialization of L. plantarum HY7714 as a probiotic with skin photo-aging inhibition, high-concentration powdering technology and maintaining shelf life stability are essential. Since functional probiotic raw materials has to maintain 1.0 ×1011CFU/g for shelf life stability, this study aimed to produce high-concentration powder of L. plantarum HY7714 and maintain stability. The number of viable cells for each process after optimization was 5.90×109cfu/ml in culture medium and 4.2×1011CFU/g in freeze-dried powder to produce high-concentration probiotic powder. As a result of the stability test, 1×1011CFU/g was maintained until 16 weeks to develop a commercial probiotic with a skin photo-aging inhibitory function. These results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin health ingredients.
Overall, it was confirmed that fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) and Lactiplantibacillus plantarum HY7714 on UVB-induced skin photo-aging inhibitory effects and could expand industrial applicability as skin health ingredients that can be safely consumed as food. Due to this, its applicability is expected to be expanded not only in the recently growing inner beauty food market, but also in the cosmetics and personal care markets.
Chronic ultraviolet (UV) light causes skin photo-aging, characterized by fine and coarse wrinkle formation and dryness. Among the native plants of Jeju-island, Gelidium amansii and Cirsium japonicum, which have excellent procollagen synthesis ability, were selected and the mixture was fermented with lactic acid bacteria to confirm the effect of preventing photo-aging of the skin. The effect of fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM) on UVB-induced photo-aging was evaluated in human dermal fibroblasts (Hs68 cell) and SKH-1 hairless mice. In vitro, FGCM increased type I procollagen levels and suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression more effectively than G. amansii and C. japonicum extract mixture (GCM). In vivo, oral administration of FGCM significantly inhibited UVB-induced the number and total depth of wrinkles in the dorsal skin of mice. FGCM suppressed UVB-induced epidermal thickening, and attenuated UVB-induced MMP-13 expression and MMP-2 and MMP-9 activities in dermal tissue. Furthermore, FGCM increased skin hydration and blocked transepidermal water loss in the dorsal skin of mice compared with the UVB-irradiated group. These data indicate that FGCM exerts potent anti-photo-aging activities by improving wrinkle formation and dryness.
Keywords: Anti-wrinkle, Cirsium japonicum, Gelidium amansii, Skin hydration, Dryness,
Fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM), Photo-aging
Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photo-aging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photo-aging. In this study, we evaluated the effect of Lactiplantibacillus plantarum HY7714 against UVB-induced photo-aging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited phosphorylation of Jun N-terminal kinase (JNK), thereby suppressing UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue.
For the industrialization of L. plantarum HY7714 as a probiotic with skin photo-aging inhibition, high-concentration powdering technology and maintaining shelf life stability are essential. Since functional probiotic raw materials has to maintain 1.0 ×1011 CFU/g for shelf life stability, this study aimed to produce high-concentration powder of L. plantarum HY7714 and maintain stability. The number of viable cells for each process after optimization was 5.90×109 CFU/mL in culture medium and 4.2×1011 CFU/g in freeze-dried powder to produce high-concentration probiotic powder. As a result of the stability test, 1×1011 CFU/g was maintained until 16 weeks to develop a commercial probiotic with a skin photo-aging inhibitory function.
Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin health ingredients.
Key words
Lactiplantibacillus plantarum, photo-aging, probiotics, ultraviolet B (UVB), Skin hydration, Anti-, Industrialization, shelf life stability, Optimization, Skin health ingredients
Keyword
#Fermented Gelidium amansii and Cirsium japonicum extract mixture (FGCM), Photo-aging, Lactiplantibacillus plantarum HY7714, , UVB, Skin Health
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