Apple bitter rot caused by Colletotrichum spp. is one of the severe diseases worldwide. Identification of Colletotrichum spp. is critical due to potential species-level differences in pathogenicity-related characteristics. To analyze the diversity of pathogen causing apple bitter rot in Korea, a sur...
Apple bitter rot caused by Colletotrichum spp. is one of the severe diseases worldwide. Identification of Colletotrichum spp. is critical due to potential species-level differences in pathogenicity-related characteristics. To analyze the diversity of pathogen causing apple bitter rot in Korea, a survey was conducted in 2020, and Colletotrichum spp. were isolated from various apple orchards in the northern Gyeongsangbuk Province, Korea. A total of 317 isolates were isolated from the infected apples showing small circular lesions light to dark brown in color, surrounded by red halos and phylogenetic relationships among the collected Colletotrichum isolates. All these isolates were identified based on morphological characteristics and analysis of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and internal transcribed spacer (ITS) genes. Molecular analyses suggested that the isolates of Colletotrichum causing the bitter rot disease in South Korea belong to seven species, which were categorized under two groups, such as Colletotrichum gloeosporioide complex which consists of 183 isolates of C. siamense species, 123 isolates of C. fructicola species, two isolates of C. aenigma, three isolates of C. gloeosporioides, and two isolates of C. conoides; while another group C. acutatum complex consists of two isolates of C. nymphaeae, and two isolates of C. acutatum. These species caused lesions on apples. Understanding the composition and characterization of the pathogen population, will help in disease management. Isolates in the C. gloeosporioides complex were analyzed using six gene regions (ACT, CHS, TUB2, ITS, GAPDH, and ApMat), and isolates in the C. acutatum complex were analyzed using partial sequencing of six genes (ACT, CHS, TUB2, ITS, GAPDH, and HIS3). The pathogenicity of these seven species demonstrated to show the confirmation of pathogenicity in the wounded the apples, while two species, C. conoides and C. nymphaeae did not show any pathogenicity on non-wounded apples.
Apple bitter rot caused by Colletotrichum spp. is one of the severe diseases worldwide. Identification of Colletotrichum spp. is critical due to potential species-level differences in pathogenicity-related characteristics. To analyze the diversity of pathogen causing apple bitter rot in Korea, a survey was conducted in 2020, and Colletotrichum spp. were isolated from various apple orchards in the northern Gyeongsangbuk Province, Korea. A total of 317 isolates were isolated from the infected apples showing small circular lesions light to dark brown in color, surrounded by red halos and phylogenetic relationships among the collected Colletotrichum isolates. All these isolates were identified based on morphological characteristics and analysis of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and internal transcribed spacer (ITS) genes. Molecular analyses suggested that the isolates of Colletotrichum causing the bitter rot disease in South Korea belong to seven species, which were categorized under two groups, such as Colletotrichum gloeosporioide complex which consists of 183 isolates of C. siamense species, 123 isolates of C. fructicola species, two isolates of C. aenigma, three isolates of C. gloeosporioides, and two isolates of C. conoides; while another group C. acutatum complex consists of two isolates of C. nymphaeae, and two isolates of C. acutatum. These species caused lesions on apples. Understanding the composition and characterization of the pathogen population, will help in disease management. Isolates in the C. gloeosporioides complex were analyzed using six gene regions (ACT, CHS, TUB2, ITS, GAPDH, and ApMat), and isolates in the C. acutatum complex were analyzed using partial sequencing of six genes (ACT, CHS, TUB2, ITS, GAPDH, and HIS3). The pathogenicity of these seven species demonstrated to show the confirmation of pathogenicity in the wounded the apples, while two species, C. conoides and C. nymphaeae did not show any pathogenicity on non-wounded apples.
주제어
#Anthracnose Apple Apple bitter rot Characterization Colletotrichum Colletotrichum acutatum complex Colletotrichum gloeosporioid-es complex Identification
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