This study identified active substances by comparing the in vitro bioactivity of Pyrrosia lingua extract and Pyrrosia lingua-derived substances, whitening efficacy through melanin synthesis factor inhibition effects in melanoma B16F10 cells of P. lingua extract and kaempferol, wrinkle improvement ef...
This study identified active substances by comparing the in vitro bioactivity of Pyrrosia lingua extract and Pyrrosia lingua-derived substances, whitening efficacy through melanin synthesis factor inhibition effects in melanoma B16F10 cells of P. lingua extract and kaempferol, wrinkle improvement effects in fibroblast CCD-986sk cells, and anti-inflammatory effects in macrophage Raw 264.7 cells were investigated. The TPC contained in the P. lingua ethanol extract was 29.38 mg/g, which was higher than that of the water extract. It was confirmed that the bioactivity effects were caused by the phenolic substance contained. The antioxidant activities for water-soluble materials were 32.0-61.4 % DPPH radical elimination ability and 74.1-99.9 % ABTS radical elimination ability, and for fat-soluble materials showed 0.87-1.27 PF value and 46.4-92.1 % TBARs inhibition, indicating similar or better activity to BHT used as a control group for the sample. In terms of tyrosinase (TYR) inhibition effects, there was no effect in water extract, but in ethanol extract, there was an effect of 7.2-40.7 %, confirming the efficacy for TYR, one of the enzymes involved in whitening. Hyaluronidase (HAase) inhibition showed an effect of 23.7-77.8 %, which showed a better inhibition effect than the control group pyrrolidine dithiocarbamate (PDTC) even at low concentrations. The inhibitory effect on elastase and collagenase showed 11.1-60.2 % elastase inhibitory effects and 10.1-100 % collagenase inhibitory effects, and sufficient effects in water extract, but very good inhibitory effect was confirmed in ethanol extract. As a result of evaluating the beauty activity of 10 types of active ingredients contained in the P. lingua extract, it was estimated that kaempferol was a substance involved in the physiological activity of the P. lingua because kaempferol was proved to be excellent.
The inhibitory effects of MITF-mediated melanin-producing signaling pathways were confirmed in B16F10 cells, toxicity to cells was not shown up to 100 μg/mL of P. lingua 80 % ethanol extract (PLE) and kaempferol 15 μM. TRP-1, TRP-2, and TYR protein expression showed inhibitory effects of 37.8 %, 40.1 %, 41.1 % and 43.0 %, 70.7 %, 26.9 % when treated at PLE 100 μg/mL and kaempferol 15 μM and concentration-dependent inhibitory activity. In addition, similar inhibitory effects were shown in MITF, TGF-β, Mc1r, and Tgfβ1 expression, which are the top factors that regulate TYR expression. The melanin formation inhibition effect was also effective in the amount of intracellular TYR, cellular melanin, Myo5a, and Rab27a expressed, and a significant decrease in melanin was confirmed. It was confirmed that melanin synthesis inhibition activity was exhibited as both PLE and kaempferol inhibited the expression of important factors during the Melanogenesis process.
As a result of confirming the down regulation effects of the skin wrinkle-induced signal transmission path in CCD-986sk cells, cell toxicity was not shown up to PLE 100 μg/mL and kaempferol 25 μM. MMP-1, MMP-9, and TIMP-1 protein expression showed inhibitory effects of 51.7 %, 29.5 %, 91.2 % and 25.3 %, 34.2 %, 100 % when treated at PLE 100 μg/mL and kaempferol 15 μM and concentration-dependent inhibitory activity. It was confirmed that the expression amount of MMP-1, MMP-2, MMP-3, MMP-9, and TIMP-1 mRNA was also significantly adjusted. Similar regulatory effects were shown in TGFβ1, Smad2, and Smad7, which are factors that control collagen biosynthesis.
As a result of investigating the effect on the expression of proteins involved in NF-κB signaling regulation in Raw 264.7 cells, depending on the treatment concentration of PLE and kaempferol, the expression of NF-κB, p-65, iNOS, and COX-2 proteins was strongly suppressed, as well as in PTGES2 and nitrite, pro-inflammatory mediators of iNOS and COX-2. It was also found that PLE and kaempferol were significantly involved in the expression of pro-inflamatory cytokines (IL-1β, IL-6, TNF-α, MCP-1).
This is a study that suggests the potential for inhibition of photoaging of kaempferol derived from PLE and PLE, active excavation of excellent biological resources native to Korea, and beneficial application in the technology development industry to increase the utilization of useful biological resources.
This study identified active substances by comparing the in vitro bioactivity of Pyrrosia lingua extract and Pyrrosia lingua-derived substances, whitening efficacy through melanin synthesis factor inhibition effects in melanoma B16F10 cells of P. lingua extract and kaempferol, wrinkle improvement effects in fibroblast CCD-986sk cells, and anti-inflammatory effects in macrophage Raw 264.7 cells were investigated. The TPC contained in the P. lingua ethanol extract was 29.38 mg/g, which was higher than that of the water extract. It was confirmed that the bioactivity effects were caused by the phenolic substance contained. The antioxidant activities for water-soluble materials were 32.0-61.4 % DPPH radical elimination ability and 74.1-99.9 % ABTS radical elimination ability, and for fat-soluble materials showed 0.87-1.27 PF value and 46.4-92.1 % TBARs inhibition, indicating similar or better activity to BHT used as a control group for the sample. In terms of tyrosinase (TYR) inhibition effects, there was no effect in water extract, but in ethanol extract, there was an effect of 7.2-40.7 %, confirming the efficacy for TYR, one of the enzymes involved in whitening. Hyaluronidase (HAase) inhibition showed an effect of 23.7-77.8 %, which showed a better inhibition effect than the control group pyrrolidine dithiocarbamate (PDTC) even at low concentrations. The inhibitory effect on elastase and collagenase showed 11.1-60.2 % elastase inhibitory effects and 10.1-100 % collagenase inhibitory effects, and sufficient effects in water extract, but very good inhibitory effect was confirmed in ethanol extract. As a result of evaluating the beauty activity of 10 types of active ingredients contained in the P. lingua extract, it was estimated that kaempferol was a substance involved in the physiological activity of the P. lingua because kaempferol was proved to be excellent.
The inhibitory effects of MITF-mediated melanin-producing signaling pathways were confirmed in B16F10 cells, toxicity to cells was not shown up to 100 μg/mL of P. lingua 80 % ethanol extract (PLE) and kaempferol 15 μM. TRP-1, TRP-2, and TYR protein expression showed inhibitory effects of 37.8 %, 40.1 %, 41.1 % and 43.0 %, 70.7 %, 26.9 % when treated at PLE 100 μg/mL and kaempferol 15 μM and concentration-dependent inhibitory activity. In addition, similar inhibitory effects were shown in MITF, TGF-β, Mc1r, and Tgfβ1 expression, which are the top factors that regulate TYR expression. The melanin formation inhibition effect was also effective in the amount of intracellular TYR, cellular melanin, Myo5a, and Rab27a expressed, and a significant decrease in melanin was confirmed. It was confirmed that melanin synthesis inhibition activity was exhibited as both PLE and kaempferol inhibited the expression of important factors during the Melanogenesis process.
As a result of confirming the down regulation effects of the skin wrinkle-induced signal transmission path in CCD-986sk cells, cell toxicity was not shown up to PLE 100 μg/mL and kaempferol 25 μM. MMP-1, MMP-9, and TIMP-1 protein expression showed inhibitory effects of 51.7 %, 29.5 %, 91.2 % and 25.3 %, 34.2 %, 100 % when treated at PLE 100 μg/mL and kaempferol 15 μM and concentration-dependent inhibitory activity. It was confirmed that the expression amount of MMP-1, MMP-2, MMP-3, MMP-9, and TIMP-1 mRNA was also significantly adjusted. Similar regulatory effects were shown in TGFβ1, Smad2, and Smad7, which are factors that control collagen biosynthesis.
As a result of investigating the effect on the expression of proteins involved in NF-κB signaling regulation in Raw 264.7 cells, depending on the treatment concentration of PLE and kaempferol, the expression of NF-κB, p-65, iNOS, and COX-2 proteins was strongly suppressed, as well as in PTGES2 and nitrite, pro-inflammatory mediators of iNOS and COX-2. It was also found that PLE and kaempferol were significantly involved in the expression of pro-inflamatory cytokines (IL-1β, IL-6, TNF-α, MCP-1).
This is a study that suggests the potential for inhibition of photoaging of kaempferol derived from PLE and PLE, active excavation of excellent biological resources native to Korea, and beneficial application in the technology development industry to increase the utilization of useful biological resources.
주제어
#Pyrrosia lingua kaempferol anti-oxidation skin aging-related genes
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