Background and Purposes: Atopic dermatitis is characterized by severe pruritus and is accompanied by dryness, ichthyosis vulgaris, hand eczema, and skin infections. Phyllostachys nigra Munro var. (PN) is traditionally known in Korean medicine to suppress inflammation of the lungs and stomach. So I t...
Background and Purposes: Atopic dermatitis is characterized by severe pruritus and is accompanied by dryness, ichthyosis vulgaris, hand eczema, and skin infections. Phyllostachys nigra Munro var. (PN) is traditionally known in Korean medicine to suppress inflammation of the lungs and stomach. So I thought that PN would be effective in suppressing inflammation even in atopic dermatitis.
Materials and Methods: BALB/c mice were divided equally into 4 groups, and 1% 1-chloro-2,4-dinitrobenzene (DNCB) (200μl) was applied continuously for 3 days as a primary sensitization to all groups except the normal group, and then From the 8th day after the 4th incubation period, 200μl of DNCB was applied to the back 7 times every 2 to 3 days for 15 days to resensitize, and on the 23rd day, all groups were sacrificed.
Scratching behavior was recorded with a Gopro Hero 4 camera, and skin lesions were measured the day before sacrifice using a modified SCORAD index.
Sectional tissues were stained with hematoxylin and eosin staining for eosinophil infiltration in tissue and thickness analysis of epidermis and dermis, and toluidine blue staining for tissue infiltration of mast cells.
ELISA was applied to analyze IgE and interleukin-6 (IL-6) in serum. To analyze the cytotoxicity of PN in HaCaT cells, an MTS assay was used. In order to determine the anti-inflammatory effect on the expression of cytokines and chemokines of PN, PN was treated at each concentration, and then tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) were again treated. In order to analyze the inhibitory effect of PN on inflammation in HMC-1 cells, PN was treated at each concentration, followed by treatment with the stimulant phorbol myristate acetate (PMA), calcium ionophore A23187 (A23187). The expression levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-1β, TNF-α, and macrophage chemotactic protein-1 (MCP-1) were analyzed using ELISA. RT-PCR was used to analyze the expression of IL-6, IL-8, thymus and activation-regulated chemokine (TARC). Western blot analysis was used to analyze the effect of PN on the phosphorylation of mitogen-activated protein kinase (MAPKs), an inflammatory signaling mechanism.
Results: PN decreased the modified SCORAD index, serum IgE, IL-6, epidermal thickness, eosinophil and mast cell infiltration. PN decreased TARC, TARC mRNA, GM-CSF, TNF-α, MCP-1, IL-6, IL-6 mRNA and phosphorylated c-Jun NH2-terminal kinase (p-JNK) phosphorylation in HaCaT cells. PN decreased GM-CSF, TNF-α, MCP-1, IL-6 in HMC-1 cells.
Conclusions: Through this study, it can be confirmed that PN has an inhibitory effect on inflammation in DNCB-induced mice, HaCaT cells and HMC-1 cells. Based on this, it is thought that PN can be used to relieve symptoms of atopic dermatitis.
Background and Purposes: Atopic dermatitis is characterized by severe pruritus and is accompanied by dryness, ichthyosis vulgaris, hand eczema, and skin infections. Phyllostachys nigra Munro var. (PN) is traditionally known in Korean medicine to suppress inflammation of the lungs and stomach. So I thought that PN would be effective in suppressing inflammation even in atopic dermatitis.
Materials and Methods: BALB/c mice were divided equally into 4 groups, and 1% 1-chloro-2,4-dinitrobenzene (DNCB) (200μl) was applied continuously for 3 days as a primary sensitization to all groups except the normal group, and then From the 8th day after the 4th incubation period, 200μl of DNCB was applied to the back 7 times every 2 to 3 days for 15 days to resensitize, and on the 23rd day, all groups were sacrificed.
Scratching behavior was recorded with a Gopro Hero 4 camera, and skin lesions were measured the day before sacrifice using a modified SCORAD index.
Sectional tissues were stained with hematoxylin and eosin staining for eosinophil infiltration in tissue and thickness analysis of epidermis and dermis, and toluidine blue staining for tissue infiltration of mast cells.
ELISA was applied to analyze IgE and interleukin-6 (IL-6) in serum. To analyze the cytotoxicity of PN in HaCaT cells, an MTS assay was used. In order to determine the anti-inflammatory effect on the expression of cytokines and chemokines of PN, PN was treated at each concentration, and then tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) were again treated. In order to analyze the inhibitory effect of PN on inflammation in HMC-1 cells, PN was treated at each concentration, followed by treatment with the stimulant phorbol myristate acetate (PMA), calcium ionophore A23187 (A23187). The expression levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-1β, TNF-α, and macrophage chemotactic protein-1 (MCP-1) were analyzed using ELISA. RT-PCR was used to analyze the expression of IL-6, IL-8, thymus and activation-regulated chemokine (TARC). Western blot analysis was used to analyze the effect of PN on the phosphorylation of mitogen-activated protein kinase (MAPKs), an inflammatory signaling mechanism.
Results: PN decreased the modified SCORAD index, serum IgE, IL-6, epidermal thickness, eosinophil and mast cell infiltration. PN decreased TARC, TARC mRNA, GM-CSF, TNF-α, MCP-1, IL-6, IL-6 mRNA and phosphorylated c-Jun NH2-terminal kinase (p-JNK) phosphorylation in HaCaT cells. PN decreased GM-CSF, TNF-α, MCP-1, IL-6 in HMC-1 cells.
Conclusions: Through this study, it can be confirmed that PN has an inhibitory effect on inflammation in DNCB-induced mice, HaCaT cells and HMC-1 cells. Based on this, it is thought that PN can be used to relieve symptoms of atopic dermatitis.
주제어
#아토피피부염, Phyllostachys nigra Munro var., Atopic dermatitis, anti-inflammation, IgE, cytokine, HaCaT, HMC-1
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