There is an ongoing safety issue with semi-permanent makeup tattoo pigments. This has led to the need for further research and accurate information about the products. This study evaluated the safety of tattoo pigments used in semi-permanent makeup, including Pigment Red 254, Pigment Orange 13, Pigm...
There is an ongoing safety issue with semi-permanent makeup tattoo pigments. This has led to the need for further research and accurate information about the products. This study evaluated the safety of tattoo pigments used in semi-permanent makeup, including Pigment Red 254, Pigment Orange 13, Pigment Green 7, and Pigment Yellow 100. The physical and chemical characteristics of the four tattoo pigments were investigated, but there was a lack of toxicity data. An MTT assay was performed to compare the cytotoxicity of 3T3, Hs68, and HaCat cells. The cell survival rate did not decrease as the concentration increased in common. Pigment Red 254 and Pigment Orange 13 showed a significant decrease in 3T3, Hs68, and HaCat cells from 1.25 μM to 10 μM, but there was no concentration-dependent decrease. Pigment Green 7 showed a decrease in cell viability of 5.63% in 3T3 cells at 2.5 μM, but it was not significant. In 1.25 μM, 5 μM, and 10 μM concentrations, the cell viability significantly decreased to 90.68%, 85.06%, and 77.54%, respectively, but there was no concentration-dependent decrease. There was an increase in cell viability of 1.17% at 1.25 μM in Hs68 cells, suggesting that there was no toxicity. At 2.5 μM, there was a 0.73% decrease in cell viability, but it was not significant. At concentrations of 5 μM and 10 μM, cell viability decreased significantly to 96.84% and 95.82%, respectively, but there was no concentration-dependent decrease. In HaCat cells, there was a 2.87% decrease in cell viability at 1.25 μM, but it was not significant. At 2.5 μM, cell viability decreased significantly to 94.99%, and at 5 μM and 10 μM, it decreased to 89.70% and 86.13%, respectively, but there was no concentration-dependent decrease. Pigment Yellow 100 did not show significant changes or concentration-dependent decreases in 3T3, Hs68, and HaCat cells at concentrations of 1.25 μM, 2.5 μM, 5 μM, and 10 μM. Based on this data, it can be concluded that Pigment Red 254, Pigment Orange 13, Pigment Green 7, and Pigment Yellow 100 have no toxic effects at concentrations from 1.25 μM to 10 μM.
There is an ongoing safety issue with semi-permanent makeup tattoo pigments. This has led to the need for further research and accurate information about the products. This study evaluated the safety of tattoo pigments used in semi-permanent makeup, including Pigment Red 254, Pigment Orange 13, Pigment Green 7, and Pigment Yellow 100. The physical and chemical characteristics of the four tattoo pigments were investigated, but there was a lack of toxicity data. An MTT assay was performed to compare the cytotoxicity of 3T3, Hs68, and HaCat cells. The cell survival rate did not decrease as the concentration increased in common. Pigment Red 254 and Pigment Orange 13 showed a significant decrease in 3T3, Hs68, and HaCat cells from 1.25 μM to 10 μM, but there was no concentration-dependent decrease. Pigment Green 7 showed a decrease in cell viability of 5.63% in 3T3 cells at 2.5 μM, but it was not significant. In 1.25 μM, 5 μM, and 10 μM concentrations, the cell viability significantly decreased to 90.68%, 85.06%, and 77.54%, respectively, but there was no concentration-dependent decrease. There was an increase in cell viability of 1.17% at 1.25 μM in Hs68 cells, suggesting that there was no toxicity. At 2.5 μM, there was a 0.73% decrease in cell viability, but it was not significant. At concentrations of 5 μM and 10 μM, cell viability decreased significantly to 96.84% and 95.82%, respectively, but there was no concentration-dependent decrease. In HaCat cells, there was a 2.87% decrease in cell viability at 1.25 μM, but it was not significant. At 2.5 μM, cell viability decreased significantly to 94.99%, and at 5 μM and 10 μM, it decreased to 89.70% and 86.13%, respectively, but there was no concentration-dependent decrease. Pigment Yellow 100 did not show significant changes or concentration-dependent decreases in 3T3, Hs68, and HaCat cells at concentrations of 1.25 μM, 2.5 μM, 5 μM, and 10 μM. Based on this data, it can be concluded that Pigment Red 254, Pigment Orange 13, Pigment Green 7, and Pigment Yellow 100 have no toxic effects at concentrations from 1.25 μM to 10 μM.
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