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Abstract ▼ AI-Helper

The present experiments were undertaken to find out the effects of ova and cummulus cells on the motiliy and movement of mouse sperms in a capillary tube modified from the microtube culture system (Cho, 1974). The results obtained were as follows: 1. The motility of the mouse sperms cultured in vitr...

The present experiments were undertaken to find out the effects of ova and cummulus cells on the motiliy and movement of mouse sperms in a capillary tube modified from the microtube culture system (Cho, 1974). The results obtained were as follows: 1. The motility of the mouse sperms cultured in vitro was decreased gradually as the culture period was prolonged or the concentration of sperms was diluted with the culture medium. 2. The ova whose cummulus cells were removed have some effects of reducing the sperm motility, but this effect seems to disappear at 8 hours of culture, whereas ova-cummulus cells complex showed a motility supprression effect only after 8 hours of culture. 3. Cummulus cells or ova-cummulus complex stimulated the movement of the sperm through a capillary tube by some degree. It is, therefore, assumed that cummulus cells secrete some factors which induce the movement of the sperm toward them.