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Abstract ▼ AI-Helper

Hydrolase zymograms of fat body in insects (Artogeia rapae, Lucillia illustris, Acrida cinerea) at various developmental stages were studied by means of electrophoresis in acrylamide gels. The results obtained are as follows; 1. Each developmental stage exhibited specific changes in zymograms of hyd...

Hydrolase zymograms of fat body in insects (Artogeia rapae, Lucillia illustris, Acrida cinerea) at various developmental stages were studied by means of electrophoresis in acrylamide gels. The results obtained are as follows; 1. Each developmental stage exhibited specific changes in zymograms of hydrolytic enzymes (esterase, acid and alkaline phosphatases) , and changes in enzyme activity of each band were observed, too. 2. A total of 12 esterase bands in Artegeia rapae, 11 in Lucillia illustris, 14 in Acrida cinerea, 6 acid phosphatase bands in Artogeia rapae, 4 inLucillia illustris, 2 in Acrida cinerea, and 8 alkaline phosphatase bands in Artogeia, rapae, 6 in Lucillia illustris, 2 in Acrida cinerea were identified, respectively. 3. In particular, hydrolase zymograms in Acrida cinerea were quite different from those of Artogeia rapae and Lucillia illustris, and were specific for each developmental stage, while the staining intensity in bands was higher in female than in male. 4. And in Acrida cinerea, acid phosphatase was separated into two bands and observed only in the direction of cathode throughout all the stages. 5. Among alkaline phosphatase bands, the most deep-dyed band was band 4 in Artogeia rapae and appeared at the 1-day old pupa of development.