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A feeding experiment was conducted using juvenile Korean rockfish (Sebastes schlegeli) to evaluate supplemental vitamin premix in a test diet containing fish meal as protein source for nutritional study. Four vitamin premixes were prepared by adjusting different quantity and combination of vitamins....

A feeding experiment was conducted using juvenile Korean rockfish (Sebastes schlegeli) to evaluate supplemental vitamin premix in a test diet containing fish meal as protein source for nutritional study. Four vitamin premixes were prepared by adjusting different quantity and combination of vitamins. Each vitamin premix contained (mg/kg diet) : premix-1 : ascorbic acid, 2666 ; a-tocopheryl acetate, 417 ; thiamin, 60 ; riboflavin, 200 ; pyridoxine, 40 ; niacin, 800 ; Ca-D-pantothenate, 280 ; myo-inositol, 4000 ; D-biotin, 6 ; folic acid, 15 ; p-amino-benjoic acid, 400 ; mennadione, 40 ; A, 16 ; $D_3$, 0.1 ; choline chloride, 8000 ; cyanocobalamin, 0.09 ; premix-2 : ascorbic acid, 270 ; a-tocopheryl acetate, 189 ; thiamin, 13.5 ; riboflavin,, 27 ; pyridoxine, 13.5 ; niacin, 135 ; Ca-D-pantothenate, 135 ; myo-inositol, 135 ; D-biotin, 1. 4 : folic acid, 4.9 ; mennadione, 5.4 : A, 5.4 ; $D_3$, 2.2 ; choline chloride, 3000 ; cyanocobalamin, 0.05 ; premix-3 : ascorbic acid, 570 ; a-tocopheryl acetate, 107 ; thiamin, 20 ; riboflavin, 14.3 ; pyridoxine, 14.3 ; niacin, 71,3 ; Ca-D-pantothenate, 57 ; myo-inositol, 456 ; D-biotin, 0.7 ; folic acid, 2.9 ; p-aminobenjoic acid, 285 ; mennadione, 5.7 ; A, 5.7 ; $D_3$, 1.1 ; choline chloride, 1500 ; cyanocobalamin, 0.03 ; premix-4 : ascorbic acid, 190 : a-tocopheryl acetate, 36 ; thiamin, 6.7 ; riboflavin, 4.8 ; pyridoxine, 4.8 ; niacin, 23.8 ; Ca-D-pantothenate, 19 ; myo-inositol, 152 ; D-biotin,0.2 ; folic acid, 1 ; p-aminobenjoic acid, 95 ; mennadione, 1.9 ; A, 1.9 ; $D_3$, 0.4 ; choline chloride, 500 ; cyanocobalamin, 0.01. Triplicate groups of the 50 fish averaging 4.25 g were fed one of four isoproteic ($47\%$) and isolipidic ($9\%$) experimental diets to satiation twice a day for 10 weeks. Weight gain, feed efficiency and protein retention in fish fed the diet with vitamin premix-1 were significantly higher than those in fish fed the other diets (P0,05). These results indicate that premix-1 can be used to adequate supplemental vitamin premix in test diet containing fish meal as protein source for juvenile Korean rockfish.

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