• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


An nprX gene of Bacillus subtilis NS15-4 encoding a neutral protease was cloned and its molecular characteristics were analyzed. The complete nucleotide sequence indicated that there is an open reading frame (0RF) possibly encoding 521 amino acid polypeptide. The ORF used all codons expected two cysteine and a proline having a codon bias index (CBI) of 0.09 in Escherichia coli. There were homologous sequences to the consensus sequence of -35 and -10 regions of E. coli promoters and to a Shine-Dalgarno (SD) sequence located 25 bp downstream of a mojor transcription initiation site. Moreover, there were also five minor transcription initiation sites at 6. 7. 8. 14 and 15 nt downstream of the major site. Northern blot analysis revealed the presence of about 1.8 kb mRNA transcript in E. coli having the nprX gene. The nucleotide sequence was identified in GenBank to be a gene for a neutral protease of B. sutilis with six nucleotide difference in the ORF region. The flanking regions of the NprX ORF showed much more differences form those of other neutral protease genes except the nprE gene of B. subtilis, which has the most homology to the nprX gene, and of which the flanking regions were identical to those of the nprX gene.

참고문헌 (21)

  1. Codon selection in yeast , Bennetzen, J.L.;B.D. Hall , J. Biol. Chem. / v.257,pp.3026-3031, 1982
  2. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases , Kawamura, F.;R.H. Doi , J. Bacteriol. / v.160,pp.442-444, 1984
  3. Gene encoding a minor extracellular protease in Bacillus subtilis , Sloma, A.;A. Ally;D. Ally;J. Pero , J. Bacteriol. / v.170,pp.5556-5563, 1988
  4. McCabe, P.C.;Innis, M.A.(ed.);D.H. Gelfaud(ed.);J.J. Swinky(ed.);T.J. White(ed.) , Production of single-stranded DNA by asymmetric PCR / v.,pp.76-83, 1990
  5. The subtilisin E gene of Bacillus subtilis in transcribed from a σ promoter in vivo , Wong, S.-L.;C.W. Price;D.S. Goldfarb;R.H. Doi , Proc. Natl. Acad. Sci. USA / v.81,pp.1184-1188, 1984
  6. Characterization of a thermostable protease from thermophilic Bacillus amyloliquefaciens NS 15-4 , Kim, H.-K.;K.-H. Kim;J.-K. Lee;Y.-O. Kim;H.-S. Nam;T.K. Oh , Kor. J. Appl. Microbiol. Biotech. / v.23,pp.322-328, 1995
  7. The use of overlapping and tailed short primers in the chromosomal assignment of short cDNAs by the polymerase chain reaction , Chae, K.-S.;K. Murakawa;K. Okubo;K. Matsubara , Gene / v.142,pp.199-205, 1994
  8. Sambrook, J.;E.F. Fritsch;T.Maniatis , Molecular Cloning: A Laboratory Manual / v.,pp., 1989
  9. Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis , Henkin, T.M.;A.L. Sonenshein , Mol. Gen. Genet. / v.209,pp.467-474, 1987
  10. Cloning and expression of a novel protease gene encoding an extracellular protease from Bacillus subtilis , Tran, L.;X.-C. Wu;S.-L. Wong , J. Bacteriol. / v.173,pp.6364-6372, 1991
  11. DNA sequencing with chain terminating inhibitors , Sanger, F.;S. Nicklen;A.R. Coulson , Proc. Natl. Acad. Sci. USA / v.74,pp.5463-5467, 1977
  12. Sequence and transcriptional analysis of the Streptomyces glaucescens tcmAR tetracenomycin C resistance and repressor gene loci , Guilfoile, P.G.;C.R. Hutchinson , J. Bacteriol. / v.174,pp.3651-3658, 1992
  13. Genetic evidence for interaction of s σA with two promoters in Bacillus subtilis , Kenney, T.J.;C.P. Moran, Jr. , J. Bacteriol. / v.173,pp.3282-3290, 1991
  14. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease , Chirgwin, J.M.;A.E. Przybyla;R.J. MacDonald;W.J. Rutter , Biochemistry / v.18,pp.5294-5299, 1979
  15. A procedure for the isolation of deoxy-ribonucleic acid from microorganisms , Marmur, J. , J. Mol. Biol. / v.3,pp.208-218, 1961
  16. Replacement of the Bacillus subtilis subtillisin structural gene with an in vitro-derived deletion mutation , Stahl, M.L.;E. Ferrari , J. Bacteriol. / v.158,pp.411-418, 1984
  17. Cloning of the neutral portease gene of Bacillus subcillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation , Yang, M.Y.;E. Ferrari;D.J. Henner , J. Bacteriol. / v.160,pp.15-21, 1984
  18. Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene , Sloma, A.;G.A. Rufo, Jr.;C.F. Rudolph;B.J. Sullivan;K.A. Theriault;J. Pero , J. Bacteriol. / v.172,pp.1470-1477, 1990
  19. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis , Moran, Jr., C.P.;N. Lang;S.F.J. LeGrice;G. Lee;M. Stephens;A.L. Sonenshein;J. Pero;R. Losick , Mol. Gen. Genet. / v.186,pp.339-346, 1982
  20. Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis , Sloma, A.;G.A. Rufo, Jr.;K.A. Theriault;M. Dwyer;S. Wilson;J. Pero , J. Bacteriol. / v.173,pp.6889-6895, 1991
  21. Cloning genetic organization, and characterization of a structural gene encoding bacillopeptidase F from Bacillus subtilis , Wu, X.-C.;S. Nathoo;A.S.-H. Pang;T. Carne;S.-L. Wong , J. Biol. Chem. / v.265,pp.6845-6850, 1990

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일