초록

섬유소자원의 이용을 목적으로 담자균류인 T. trogii를 실험 균주로 하여 합성배지에서 cellulase 생성 최적 배양조건을 검토해 본 결과는 다음과 같다. 합성배지에서 T. trogii에 의한 cellulase 생산은 $30{\sim}35^{\circ}C,\;pH\;4.0{\sim}6.0,\;and\;11{\sim}15$일 배양시 가장 높았다. 탄소원은 Avicelase와 ${\beta}-glucosidase$의 경우 CMC를, CMCase는 cellulose를 탄소원으로 했을 때 최고의 생산성을 보였으며, CMC의 최적 농도는 세 효소 모두 3%이었다. 질소원은 ammonium tartrate 첨가시 cellulase 생산이 높았으며, 그 최적 농도로 Avicelase와 ${\beta}-glucosidase$는 0.03N, CMCase는 0.04 N이었다.

Abstract

For the purpose of utilizingcellulosesresources by cellulolytic enzymes of Trametes trogii, its cultural conditions for the production of cellulolytic enzymes in synthetic media were investigated. The optimum conditions for the production of cellulase by T. trogii in synthetic media were $30{\sim}35^{\circ}C,\;pH\;4.0{\sim}6.0,\;and\;11{\sim}15$ day's cultivation. Among the carbon sources, carboxymethyl cellulose was good for the production of avicelase and ${\beta}-glucosidase$, but cellulose was good for the production of CMCase. The optimum concentration of Na-CMC was 3% for the production of all the three cellulolytic enzymes. As the nitrogen source, $0.03{\sim}0.04%$ N as ammonium tartrate was effective for the production of the cellulases.

주제어

#Trametes trogii   #cellulase   #cultural conditions  

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