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Abstract ▼ AI-Helper

This study was carried out to increase the rate of plant regeneration from embryogenic callus of A. keiskei on MS medium supplemented with ethylene inhibitors. When leaflet, petiolule, and petiole of A. keiskei were cultured on MS medium supplemented with 2, 4-D, callus was well induced from leaflet...

This study was carried out to increase the rate of plant regeneration from embryogenic callus of A. keiskei on MS medium supplemented with ethylene inhibitors. When leaflet, petiolule, and petiole of A. keiskei were cultured on MS medium supplemented with 2, 4-D, callus was well induced from leaflet segments at 2.0 ppm 2, 4-D. Shoot elongation of plantlets and shooting from embryogenic callus of A. keiskei were best on 2, 4-D-free medium supplemented with 2 ppm $AgNO_3$ or 10 ppm $CoCl_2{\cdot}6H_2O$, but it was suppressed on the medium containing 1 ppm 2, 4-D with $AgNO_3$ or $CoCl_2{\cdot}6H_2O$. Root elongation of plantlets from embryogenic callus was best on 2, 4-D-free medium supplemented with 1 ppm $AgNO_3$ or 5 ppm $CoCl_2{\cdot}6H_2O$, but rooting from embryogenic callus was none on the medium containing 1 ppm 2, 4-D with $AgNO_3$ or $CoCl_2{\cdot}6H_2O$. Fresh weight of plantlets from embryogenic callus was heaviest on 2, 4-D-free medium supplemented with 2 ppm of $AgNO_3$ or $CoCl_2{\cdot}6H_2O$, while it was heaviest on the medium containing 1 ppm 2, 4-D with 1 ppm $AgNO_3$ or 2 ppm $CoCl_2{\cdot}6H_2O$.

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