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Abstract ▼ AI-Helper

Haemolymph ferritin of H. cunea was found to have M.W of 660 kDa, three subunits of 26, 30 and 32 kDa, and pI value of 7.3. The NH$_2$-terminal amino acid sequence of major subunit 26kDa was.PQ.HINPV and was not similar to those of another animals. And H. cunea ferritin showed 7 nm partic...

Haemolymph ferritin of H. cunea was found to have M.W of 660 kDa, three subunits of 26, 30 and 32 kDa, and pI value of 7.3. The NH$_2$-terminal amino acid sequence of major subunit 26kDa was.PQ.HINPV and was not similar to those of another animals. And H. cunea ferritin showed 7 nm particle by bismuth stain of electron microscope and did not represent the cross- reactivity to other four insects (Lepidoptera) as well as horse spleen ferritin. Haemolymph ferritin of H. cunea was distributed in several organs such ag gut, fat body, Malphigian tubules, muscle, and trachea but not in integument. In iron- fed larva by adding FeC1$_3$directly to the diet, organs such as haemolymph, fat body, gut and Malphigian tubules showed the concentration fluctuation of ferritin. Ferritin level in fat body increased 1 and 2 days but decreased below the level of control diet 3 days after iron-feeding, whereas ferritin concentration in haemolymph didn't show any changes until 2 days but increased 3 days after iron- feeding.

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