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제안 방법
- Oriental melon plants inoculated with potato dextrose broth and an isolate M9209 were included in each run of vimlence test for negative and positive controls, respectively. The experiments were conducted as a randomized complete block design with 5 replicates and 3 plants per replicate.
대상 데이터
- Generation and phenotypes of nit mutants. Prior to generate nit mutants from 90 isolates, 5 isolates (M9209, MKA-1, MKH-W, MCK04, and MNSH-8) were tested on MM with varying concentrations of KC1O3 (1.5, 2.0, 2.5, 3.0, and 3.5%). The highest frequency of NitM was obtained on MM containing 3.
- melonis. Seven to 21 nit mutants were obtained from each isolate. The phenotypes of the nit mutants were determined by their colony morphology on MM containing nitrate, nitrite, hypoxanthine, uric acid or ammonium tartrate as the sole nitrogen source.
데이터처리
- complete wilting and plant death. Mean followed by the same letter within a row are not significantly different, using Duncan's multiple range tests(P = 0.05).
이론/모형
- The mycelial mats were removed by passing through 4 layers of cheesecloth, and the conidial concentration was adjusted to 5xl05 conidia per ml. Virulence test of all isolates was performed on the susceptible melon cultivar 'Eunsung' VM-Tv-1 using the root dip method described by Jacobson and Gordon (1988). Plant seedlings were grown in soil mix (vermiculite : perlite : peat moss = 1 : 1 : 1) at 22-28℃ for 2 weeks until the third true leaf began to emerge.
- Vegetative compatibility. Nitrate nonutilizing (nit) mutants were generated and the phenotypes of all nit mutants were determined by the method of Correll et al, (1987). Stable nil! and NitM phenotypes from each isolates were stored in the sterile distilled water at 4℃.
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