RAPDs 분석에 의한 양친의 유전적 유사성 정도가 F1잡종강세 예측에 효율적으로 이용될 수 있는지 알아보고자 통일형 6 품종(밀양42, IR747, 백운찰벼, 삼강벼, 수원307, 이리356) 간 반이면 교배 F1 15조합을 작성하였다. 양친과 F1을 공시하여 조사한 수량 등 8개의 특성과 RAPDs 분석을 통해 구한 양친의 유전적거리와의 관계를 조사하였다. 120개의 Operon 프라이머를 6개 품종의 DNA 증폭에 이용한 결과 3367개의 밴드가 발생되었으며 이중 168개가 6개 품종 중 1개 이상의 품종에서 변이를 보였다. 6품종간 유전적 거리는 최소 0.157(백운찰벼와 수원 307호 간), 최대 0.383(삼강벼와 이리356호 간)의 분포를 보였다. 잡종강세의 정도는 정조수량(129%), 수당립수(125%), 수장(109%) 등이 높은 잡종강세를 보였고, 유전적거리는 수당립수와 통계적으로 유의한 부의 상관을 보였지만, 정조수량, 천립중 등과는 유의한 상관을 보이지 않았다.
RAPDs 분석에 의한 양친의 유전적 유사성 정도가 F1 잡종강세 예측에 효율적으로 이용될 수 있는지 알아보고자 통일형 6 품종(밀양42, IR747, 백운찰벼, 삼강벼, 수원307, 이리356) 간 반이면 교배 F1 15조합을 작성하였다. 양친과 F1을 공시하여 조사한 수량 등 8개의 특성과 RAPDs 분석을 통해 구한 양친의 유전적거리와의 관계를 조사하였다. 120개의 Operon 프라이머를 6개 품종의 DNA 증폭에 이용한 결과 3367개의 밴드가 발생되었으며 이중 168개가 6개 품종 중 1개 이상의 품종에서 변이를 보였다. 6품종간 유전적 거리는 최소 0.157(백운찰벼와 수원 307호 간), 최대 0.383(삼강벼와 이리356호 간)의 분포를 보였다. 잡종강세의 정도는 정조수량(129%), 수당립수(125%), 수장(109%) 등이 높은 잡종강세를 보였고, 유전적거리는 수당립수와 통계적으로 유의한 부의 상관을 보였지만, 정조수량, 천립중 등과는 유의한 상관을 보이지 않았다.
In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agrono...
In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.
In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.
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문제 정의
These include “Milyang42", “IR747”, "Baekunchalbyeo", "Samgangbyeo", “Suweon30 7", and "Iri356” (Table 1). A non-reciprocal set of 15 crosses among six varieties was made and the 15 Fl hybrids along with the six parents were grown for phenotypic' evaluation in a field. The field planting followed a randomized complete block design with three replications.
가설 설정
many plant species. The proposal that the level of genetic diversity might be useful as a predictor of Fi performance and heterosis is based on the finding that heterosis was associated with the interaction of different alleles at a locus (3). Restriction fragment length polymorphisms (RFLPs) have been employed to examine genetic diversity and its「이ationship to heterosis in maize (9, 16), and soybean (4).
제안 방법
In this study, we examined genetic divergence among six Tongil-type cultivars with RAPDs and assessed the relationship between molecular polymorphism and hybrid performance. The poor association between marker-based diversity and heterosis found in this study might need further consideration of the usefulness of DNA markers for selecting for hybrid combinations among Tongil-type cultivars, and require testing of more genotypes in hybrid combinations.
A non-reciprocal set of 15 crosses among six varieties was made and the 15 Fl hybrids along with the six parents were grown for phenotypic' evaluation in a field. The field planting followed a randomized complete block design with three replications. Each plot consisted of twenty-five plants planted in a single row with 15-cm spacing between plants and 30-cm spacing between rows.
This study was carried out using RAPDs markers to assess the genetic diversity among six Tongil-type varieties widely used in rice breeding programs and to evaluate the association of the genetic diversity with Fl performance and heterosis whether diversity at the molecular level could be useful for predicting Fi performance.
대상 데이터
Six Tongil-type varieties commonly used as the parents in programs aimed at developing high yielding cultivars were selected for this study. These include “Milyang42", “IR747”, "Baekunchalbyeo", "Samgangbyeo", “Suweon30 7", and "Iri356” (Table 1).
이론/모형
Means over replications were calculated for each trait and used in data analysis. Traits were evaluated based on the Manual for Standard Evaluation Methods of Rural Development Administration (RDA) (14).
성능/효과
Each plot consisted of twenty-five plants planted in a single row with 15-cm spacing between plants and 30-cm spacing between rows. The traits evaluated were days to heading, culm length (cm), panicle length (cm), panicles per plant, spikelets per panicle, 1,000-grain weight (grams), ripening ratio (%), and grain yield in milled rice (t , ha-1). Means over replications were calculated for each trait and used in data analysis.
후속연구
performance. The poor association between marker-based diversity and heterosis found in this study might need further consideration of the usefulness of DNA markers for selecting for hybrid combinations among Tongil-type cultivars, and require testing of more genotypes in hybrid combinations. In this regard, studies including Tongil-type/indica and tropical japonica hybrids are needed to establish the relationship between marker heterozygosity and heterosis and evaluate the possibility of exploiting the heterosis for those crosses.
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