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cDNA Sequence and mRNA Expression of a Novel Peroxiredoxin from the Firefly, pyrocoelia rufa 원문보기

International journal of industrial entomology, v.4 no.2, 2002년, pp.101 - 107  

Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University, Busan 604-714) ,  Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University, Busan 604-714) ,  Kim, Seong-Ryul (College of Natural Resources and Life Science, Dong-A University, Busan 604-714) ,  Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University, Busan 604-714)

Abstract ▼ AI-Helper 아이콘AI-Helper

We describe here the cDNA sequence and mRNA expression of a novel family of the antioxidant protein, peroxiredoxin, from the firefly, Pyracoetia ruin. The 555 bp cDNA sequence codes for a 185 amino acid protein with a calculated molecular mass of approximately 21 kDa. The deduced protein of P. rufa ...

주제어

#Insect   #Firefly   #Antioxidant protein   #Peroxiredotin   #pyrocoelia rufa   #cDNA sequence   #Phylogeny  

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제안 방법

  • Sequence of each cDNA clone was determined using an automatic sequencer (model 310 Genetic Analyzer; Perkin-Elmer Applied Biosystems, Foster City, CA). 1'he sequences were compared using the DNASIS and BLAST programs provided by the NCBL GenBank, EMBL and SwissProt databases were searched for sequence homology using a BLAST algorithm program.
  • , 2000). The sequencing of randomly selected clones harboring cDNA inserts was performed to generate the R rufa ESTs. Of these ESTs, one exhibited similarity to the reported Prx genes.
  • rufa larvae. The sequencing of randomly selected clones harboring cDNA inserts was performed to generate the expressed sequence tags (ESTs). For DNA sequencing, plasmid DNA was extracted by Wizard mini-preparation kit (Promega).

대상 데이터

  • The larvae of the firefly, Pyrocoelia rufa, were collected at Miryang, Kyungnam province in Korea. The live larvae were directly used in this study.

이론/모형

  • 5) was used to align the amino acid sequences of Prx gene. Including the eighteen GenBank- registered amino acid sequences of Prx genes, phylogenetic analysis among Prx genes was performed using PAUP (Phylogenetic Analysis using Parsimony) version 3.0 (Swofford, 1990). The tree was obtained by bootstrap analysis with the option of heuristic search (1,000 replications).
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