The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release ind...
The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release induced by compound 48/80 in mice. In addition, SRAE also inhibited the passive cutaneous anaphylaxis reaction induced by IgE/anti-IgE in mice. The effect of SRAE on the histamine release from rat peritoneal mast cells (RPMC) was studied. SRAE inhibited the histamine release induced by compound 48/80 in RPMC. To clarify the mechanism of these inhibiting reactions, we investigated the effects of SRAE on cyclic AMP (cAMP). The level of cAMP in human leukemia cell line, HMC-1, when SRAE (1 mg/ml) was added, significantly increased compared with that of basal cells. These results indicate that SRAE may be beneficial in the treatment of immediate-type allergic reaction.
The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release induced by compound 48/80 in mice. In addition, SRAE also inhibited the passive cutaneous anaphylaxis reaction induced by IgE/anti-IgE in mice. The effect of SRAE on the histamine release from rat peritoneal mast cells (RPMC) was studied. SRAE inhibited the histamine release induced by compound 48/80 in RPMC. To clarify the mechanism of these inhibiting reactions, we investigated the effects of SRAE on cyclic AMP (cAMP). The level of cAMP in human leukemia cell line, HMC-1, when SRAE (1 mg/ml) was added, significantly increased compared with that of basal cells. These results indicate that SRAE may be beneficial in the treatment of immediate-type allergic reaction.
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대상 데이터
2000. A voucher specimen (number WSP-00-17) was deposited at the Herbarium of the College of Pharmacy, Woosuk University. The plant sample was extracted with distilled water at 70℃ for 5 h.
Compound 48/80, anti-DNP IgE, DNP-human serum albumin (HSA), oc-minimal essential medium (a-MEM), ortho-phthaldialdehyde and metrizamide were purchased from Sigma Chemical Co. (St Louis, MO). cAMP was purchased from Amersham Pharmacia Biotec (UK).
The original stock of ICR mice and SD rats were purchased from Dae-Han Biolink (Chungbuk), and the animals were maintained in the College of Pharmacy, Wbosuk University. The animals were housed five to ten per cage in a laminar air flow room maintained under a temperature of 22±2℃and relative humidity of 55±5% throughout the study.
데이터처리
mean±SEM. Student's t-test was used to make a statistical comparison between the groups. Results with p<0.
성능/효과
The results of this study demonstrated that SRAE pretreatment profoundly inhibited compound 48/80-induced systemic anaphylaxis and anti-DNP IgE-mediated PCA. SRAE inhibited the plasma histamine levels in mice.
후속연구
Also, it suggests that SRAE may contain compounds with actions that inhibit mast cell- mediated allergic reactions in vivo and in vitro. Therefore, further investigation is necessary to clarify unknown anti-anaphylactic constituents that may be more active than the SRAE itself.
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