[논문]Anti-Inflammation Activity of Actinidia polygama

Kim, Yoo-Kyung; Kang, Hyo-Joo; Lee, Kyung-Tae; Choi, Jin-Gyu; Chung, Sung-Hyun

doi:10.1007/bf02994759

[국내논문] Anti-Inflammation Activity of Actinidia polygama 원문보기

Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, v.26 no.12, 2003년, pp.1061 - 1066

Kim, Yoo-Kyung (Laboratory of Pharmacology) , Kang, Hyo-Joo (Laboratory of Pharmacology) , Lee, Kyung-Tae (Biochemistry, School of Pharmacy, Kyung Hee University) , Choi, Jin-Gyu (Korea Herbal Science Institute) , Chung, Sung-Hyun (Laboratory of Pharmacology)

Abstract ▼ AI-Helper

The fruit of Actinidia polygama (AP) has long been used as a folk medicine in Korea for treating pain, rheumatic arthritis and inflammation. The present investigation was carried out to determine the in vivo and in vitro anti-inflammatory activity of AP using several animal models of inflammation. The 70% ethanol extract of the fruit of AP significantly inhibited acetic acidinduced, vascular permeability in a dose dependent manner (23%, 38%, and 41 % inhibition at doses of 200 mg/kg, 500 mg/kg and 1000 mg/kg, respectively). This effect was maintained in AP water-soluble fraction (APW). The APW fraction also showed significant inhibitory activity against the rat paw edema induced by a single treatment of carrageenan. In vitro experiments were performed to demonstrate the inhibitory activities of APW (100 $\mu$ g/ml) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production. The results showed that APW dose-dependently suppressed LPS-induced NO production in RAW 264.7 macrophages without a notable cytotoxic effect and also decreased inducible NO synthase (iNOS) protein expression. APW also showed a significant inhibitory effect in LPS-induced $PGE_2$ production and cyclooxygenase-2 (COX-2) expression.

Keyword

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

In this study, we evaluated the anti-inflammatory effect of the 70% ethanol extract of AP using an acetic acid-induced, vascular permeability test. After solvent fractionation, we compared the anti-inflammatory effects of AP ether and water-soluble fractions (APE and APW).
This finding prompted us to conduct in vitro experiments into the anti-inflammatory action of this plant material to determine its anti-inflammatory mechanism. We evaluated the effects of APW on LPS-induced NO, PGE2 release by the macrophage cell line RAW 264.7 and examined the protein expression of iNOS and COX-2.
In accordance with the ethnomedical use of AP, the present study was carried out to evaluate the anti-inflammatory activity of the ethanol extract of this plant and to identify the active fraction from the extract for further isolation of the active principle(s).

대상 데이터

Male Sprague-Dawley rats (160~200 g, Han-lim Lab. animal, Hwasung, Korea) and male ICR mice (25-30 g, Han-lim Lab. animal, Hwasung, Korea), aged 6 weeks, were used. All animals were acclimatized to the laboratory environment for one week before the experiment.

데이터처리

All data are expressed as mean± SEM. The data were analyzed using Students t-test. Mean values were considered significantly different at P<0.

참고문헌 (9)

Harris, S. G., Padilla, J., Koumas, L., Ray, D., and Phipps, R. P., Prostaglandins as modulators of immunity. Trends. Immunol., 23(3),144-150 (2002)

상세보기
Herschman, H.R., Prostaglandin synthase 2. Biochem. Biophys. Acta., 1299(1), 125-140 (1996)

상세보기
Kapu, S. D., Ngwai, Y. B., Kayode, O., Akah, P.A.., Wambebe, C., and Gamaniel, K., Anti-inflammatory, analgesic and antilymphocytic activities of the aqueous extract of Crinum giganteum. J. Ethnopharmacol., 78, 7-13 (2001)

상세보기
Kim, J. S., Kim, J. M., Jung, H. C., and Song, I. S., Expression of cyclooxygenase-2 in human neutrophils activated by helicobacter pylori water-soluble proteins: possible involvement of NF-kappaB and MAP kinase signaling pathway. Dig. Dis. Sci., 46(10), 2277-2284 (2002)

상세보기
Lee, E. B., Li, D.W., Hyun, J. E., Kim, I. H., and Whang, W. K., Anti-inflammatory activity of methanol extract of Kalopanx pictus bark and its fractions. J. Ethnopharmacol., 77, 197-201 (2001)

상세보기
Lee, S. H. , Soyoola, E., Chanmugam, P., Hartt, S., Sun, W., Zhong, H., Liou, S., Simmons, D., and Hwang, D., Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J. Biol. Chem., 267(36), 25934-25938 (1992)
Olajide, O. A., Awe, S. O., Makinde, J. M., Ekhelar, A. I., Olusola. A., Morebise, O., and Okpako, D. T., Studies on the anti inflammatory, antipyretic and analgesic properties of Alstonia boonei stem bark. J. Ethnopharmacol., 71(1-2), 179-186 (2000)

상세보기
Srinivasan, K., Muruganandan, S., Lal, J., Chandra, S., Tandan, S. K., and Prakash, V. R., Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J. Ethnopharmacol., 78(2-3), 151-157 (2001)

상세보기
Vane, J. R. and Botting, R. M., Anti-inflammatory drugs and their mechanism of action. Inflamm Res., 2, S78-87. (1998)

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증