In the present study, we carried out comparative research on the anthraquinones Raman spectrum and on the anthraquinones derivative 1,4-diamino-anthraquinone focusing on change in its intermediate in terms of pH and change in the substituent. WE use the SERS method and employ a silver sol prepared by Creighton et al.'s method. From the analysis of the UV spectrum of the mixture solution of 1,4-diamino-anthraquinone and silver sol, we could see that the 1,4-diamino-anthraquinone physically adsorbs silver sol. In terms of the adsorbing orientation, the adsorption of the nitrogen atom in the amino group is perpendicular to the surface of silver sol according to the surface selection rule. From the structure of the 1,4-diamino-anthraquinone intermediate according to the change of pH, we could see that the C=O bond is strengthened in the acidic state and weakened in the neutral and the alkaline state because of the resonance effect of the amines.