[논문]Conversion of G. hansenii PJK into Non-cellulose-producing Mutants According to the Culture Condition

Park, Joong-Kon; Hyun, Seung-Hun; Jung, Jae-Yong

doi:10.1007/bf02933062

Conversion of G. hansenii PJK into Non-cellulose-producing Mutants According to the Culture Condition 원문보기

Biotechnology and bioprocess engineering : Bbe, v.9 no.5, 2004년, pp.383 - 388

Park, Joong-Kon (Department of Chemical Engineering, Kyungpook National University) , Hyun, Seung-Hun (Department of Chemical Engineering, Kyungpook National University) , Jung, Jae-Yong (Department of Chemical Engineering, Kyungpook National University)

Abstract ▼ AI-Helper

The conversion of a cellulose-producing cell ($Cel^+$) from Gluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell ($Cel^-$) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion of microbial cells from a wild type to $Cel^-$ mutants in a flask culture. The supplementation of $1\%$ ethanol to the medium containing an organic acid depressed the con-version of the microbial cells to $Cel^-$ mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid; however, accelerated the conversion of microbial cells in the flask with slanted baffles. The $Cel^+$ cells from the agitated culture were not easily converted into $Cel^-$ mutants on the additions of organic acid and ethanol to a flask without Slanted baffles, but some portion of the $Cel^+$ cells were converted to $Cel^-$ mutants in a flask with slanted baffles. The conversion ratio of $Cel^+$ cells to $Cel^-$ mutants was strongly re-lated to the production of bacterial cellulose independently from the cell growth.

주제어

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문제 정의

The conversion ratio of microbial cells to Cel^- mutants was strongly related to the production of bacterial cellulose. The focus of this study was not directed towards the industrial mass production of BC, but this result will be a good guideline for developing technology to effectively produce BC on an industrial scale.

가설 설정

7 wm, as shown in Fig. 1. This means that the oxygen transferred from the air bubbles to the liquid medium was completely consumed by microorganisms, and the cell growth was accelerated by the large amount of oxygen supplied. This resulted in a high cell density in the fermentor, which was about 33% larger than the shaking culture, as shown in Table 2.

제안 방법

production of BC in an agitated cultivation. In this study, we tried to investigate the effect of operating conditions including the shear stress generated at the slanted baffles on the flask wall on the conversion of G/u-conacetobacter hansenii PJK cells and BC production as the basic research in order to obtain optimum fermentation condition in the future study for BC production in an agitated vessel.

성능/효과

The addition of acetic acid to the basal medium (and thus, MA) induced the conversion of microorganisms to Cel~ mutants, and the addition of glutamic acid (and thus, MG) accelerated the conversion of microorganisms. This lead to greater BC production in the, MA than the MG medium, as shown in Table 2. The supplementation of 1% ethanol to the MA medium (and thus, MAE) efficiently prevented (nearly completely) the conversion of microorganisms to Cel~ mutants in the conventional flask culture. The addition of 1% ethanol to the MG medium (and thus, MGE) partially prevented the conversion of microbial cells and reduced the conversion ratio from 0.
Thus, at this stage, how the conversion of Cel+ cells from wild type to Cel^- mutants occurs was investigated in relation to the culture conditions. The experimental results seemed to be slightly cumbersome but a clear trend could be found. In a one-dimensional circulating medium flow, organic (acetic acid) and amino (glutamic acid) acids induced the conversion of microbial cells, and the supplementation of ethanol seemed to prevent the conversion of microbial cells.

후속연구

Accordingly, further research is required to investigate the optimum fermentation conditions, including nutrient components and medium mixing modes, in an incubator producing a large amount of eddies, resulting in the minimum conversion rate of microbial strains and the maximum production of BC in an agitated cultivation. In this study, we tried to investigate the effect of operating conditions including the shear stress generated at the slanted baffles on the flask wall on the conversion of G/u-conacetobacter hansenii PJK cells and BC production as the basic research in order to obtain optimum fermentation condition in the future study for BC production in an agitated vessel.

참고문헌 (19)

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DOI : 10.1007/BF02933062 [무료]
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