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The conversion of a cellulose-producing cell ($Cel^+$) from Gluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell ($Cel^-$) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion of microbial cells from a wild type to $Cel^-$ mutants in a flask culture. The supplementation of $1\%$ ethanol to the medium containing an organic acid depressed the con-version of the microbial cells to $Cel^-$ mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid; however, accelerated the conversion of microbial cells in the flask with slanted baffles. The $Cel^+$ cells from the agitated culture were not easily converted into $Cel^-$ mutants on the additions of organic acid and ethanol to a flask without Slanted baffles, but some portion of the $Cel^+$ cells were converted to $Cel^-$ mutants in a flask with slanted baffles. The conversion ratio of $Cel^+$ cells to $Cel^-$ mutants was strongly re-lated to the production of bacterial cellulose independently from the cell growth.

참고문헌 (19)

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이 논문을 인용한 문헌 (5)

  1. 2005. "" Biotechnology and bioprocess engineering, 10(4): 289~295 
  2. 2005. "" Biotechnology and bioprocess engineering, 10(1): 1~8 
  3. 2008. "" Biotechnology and bioprocess engineering, 13(2): 240~247 
  4. Khan, Salman ; Shehzad, Omer ; Khan, Taous ; Ha, Jung Hwan ; Park, Joong Kon 2009. "Production of Bacterial Cellulose by Gluconacetobacter hansenii Using a New Bioreactor Equipped with Centrifugal Impellers" 화학공학 = Korean chemical engineering research, 47(4): 506~511 
  5. 2010. "" Biotechnology and bioprocess engineering, 15(1): 110~118 


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