Yim Joung Han
(Korea Polar Research Institute, KORDI)
,
Kim Sung Jin
(Korea Polar Research Institute, KORDI)
,
Aan Se Hoon
(Korea Polar Research Institute, KORDI)
,
Lee Hong Kum
(Korea Polar Research Institute, KORDI)
The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ 10356 were investigated. The $E_{24}$ of $0.5\%$ EPS-R was $89.2\%$, which was higher than that observed in commercial polysaccharides such as x...
The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ 10356 were investigated. The $E_{24}$ of $0.5\%$ EPS-R was $89.2\%$, which was higher than that observed in commercial polysaccharides such as xanthan gum ($67.8\%$), gellan gum ($2.01\%$) or sodium alginate ($1.02\%$). Glucose and galactose are the main Sugars in EPS-R, with a molar ratio of ${\~}1:6.8$, xylose and ribose are minor sugar components. The average molecular mass, as determined by gel filtration chromatography, was $2.2{\times}10^3$ KDa, The intrinsic viscosities of EPS-R were calculated to be 16.5 and 15.9 dL/g using the Huggins and Kraemer equations, respectively, with a 2.3 dL/g overlap. In terms of rigidity, the conformation of EPS-R was similar to that of caboxymethyl cellulose ($5.0{\times}10^{-2}$). The rheological behavior of EPS-R dispersion indicated that the formation of a structure intermediate between that of a random-coil polysaccharide and a weak gel. The aqueous dispersion of EPS-R at concentrations ranging from 0.25 to $1.0\%$ (w/w) showed a marked shear-thinning property in accordance with Power-law behavior. In aqueous dispersions of $1.0\%$ EPS-R, the consistency index (K) and flow behavior index (n) were 1,410 and 0.73, respectively. EPS-R was Stable to pH and salts.
The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ 10356 were investigated. The $E_{24}$ of $0.5\%$ EPS-R was $89.2\%$, which was higher than that observed in commercial polysaccharides such as xanthan gum ($67.8\%$), gellan gum ($2.01\%$) or sodium alginate ($1.02\%$). Glucose and galactose are the main Sugars in EPS-R, with a molar ratio of ${\~}1:6.8$, xylose and ribose are minor sugar components. The average molecular mass, as determined by gel filtration chromatography, was $2.2{\times}10^3$ KDa, The intrinsic viscosities of EPS-R were calculated to be 16.5 and 15.9 dL/g using the Huggins and Kraemer equations, respectively, with a 2.3 dL/g overlap. In terms of rigidity, the conformation of EPS-R was similar to that of caboxymethyl cellulose ($5.0{\times}10^{-2}$). The rheological behavior of EPS-R dispersion indicated that the formation of a structure intermediate between that of a random-coil polysaccharide and a weak gel. The aqueous dispersion of EPS-R at concentrations ranging from 0.25 to $1.0\%$ (w/w) showed a marked shear-thinning property in accordance with Power-law behavior. In aqueous dispersions of $1.0\%$ EPS-R, the consistency index (K) and flow behavior index (n) were 1,410 and 0.73, respectively. EPS-R was Stable to pH and salts.
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제안 방법
Sugar spots were visualized by spraying the plates with a solution of aniline-phthalic acid in wa ter-saturated butanol, followed by incubation at 100°C for 5 min. HPLC analysis was performed on a YMC-Pack NH2 column (4.6 x 250 mm YMC Co., Japan). Elusion was performed with water-acetonitrile (15:85) at a flow rate of 1.
The analysis of the polysaccharide chemical group characteristics from the infrared spectrum of EPS-R showed -OH stretching at -3, 400 cm'1, C-H stretching at 〜2, 900 cm1, stretching vibrations of the carboxylate group at 〜1, 600 cm'1 and the amine group (-NH3) at 1560 cm1, C-O-C antisymmetrical stretching or a polysaccharide sugar ring at -1, 000 cm1, and CO2 in air at 2, 400 cm ', which was similar to typical polysaccharide IR spectra (Fig. 2).
대상 데이터
The marine bacterium strain 96CJ10356 was used and deposited as Hahella chejuensis (KCTC 2396T = IMSUN 1 157t). A culture was grown in STN medium containing 20 g sucrose, 10 g tryptone, 10 g NaCl, 5 g MgSO4) 1 g CaCl2, 83 mg KH2PO4, 67 mg K2HPO4, 5 mg FeCl2) 1 mg MnCl2) 1 mg ZnCl2 and 1 mg NaMoO4 per liter at pH 7.
이론/모형
Agitation was maintained overnight at 4°C. The concen tration of the EPS-R stock solution was determined by the phenol-sulfuric acid method [23]. This stock solution was diluted to the desired concentration (ranging from 10 to 0.
4. The intrinsic vis cosities of EPS-R were 16.5 and 15.9 응/dL, as calculated using the Huggins equation and the Kraemer equation, respectively. The intrinsic viscosity of EPS-R was v\ (i.
성능/효과
In conclusion, EPS-R is a novel emulsifier produced by the marine bacterium Hahella chejuensis strain 96CJ10356. Studies on the yield optimization will be requiring before estimations of its commercial potential can be made.
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