[논문]Self-Assembled Polymeric Nanoparticles of Poly(ethylene glycol) Grafted Pullulan Acetate as a Novel Drug Carrier

Jung, Sun-Woong; Jeong, Young-Il; Kim, Young-Hoon; Kim, Sung-Ho

doi:10.1007/bf02980132

Self-Assembled Polymeric Nanoparticles of Poly(ethylene glycol) Grafted Pullulan Acetate as a Novel Drug Carrier 원문보기

Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, v.27 no.5, 2004년, pp.562 - 569

Jung, Sun-Woong (College of Pharmacy, Chosun University) , Jeong, Young-Il (Research Institute of Medical Sciences, Chonnam National University Medical Schoo) , Kim, Young-Hoon (Department of Chemistry, University of Massachusetts at Lowel) , Kim, Sung-Ho (College of Pharmacy, Chosun University)

Abstract ▼ AI-Helper

Self-assembling nanospheres of hydrophobized pullulan have been developed. Pullulan acetate (PA), as hydrophobized pullulan, was synthesized by acetylation. Carboxymethylated poly(ethylene-glycol) (CMPEG) was introduced into pullulan acetate (PA) through a coupling reaction using N, N'-dicyclohexyl carbodiimide (DCC). A synthesized PA-PEG-PA (abbreviated as PEP) conjugate was confirmed by Fourier transform-infrared (FT-IR) spectroscopy. Since PEP conjugates have amphiphilic characteristics in aqueous solution, polymeric nanoparticles of PEP conjugates were prepared using a simple dialysis method in water. From the analysis of fluorescence excitation spectra primarily, the critical association concentration (CAC) of this conjugate was found to be 0.0063 g/L. Observations by scanning electron microscopy (SEM) showed the spherical morphologies of the PEP nanoparticles. The particle size distribution of the PEP conjugates was determined using photon correlation spectroscopy (PCS) and the intensity-average particle size was 193.3 ${\pm}$ 13.53 nm with a unimodal distribution. Clonazepam (CNZ), as a model drug, was easy to entrap into polymeric nanoparticles of the PEP conjugates. The drug release behavior was mainly diffusion controlled from the core portion.

주제어

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문제 정의

Clonazepam (CNZ), as a model dnjg, was easy to entrap into polymeric nanoparticles of PEP conjugates and the drug release rate was increased by intcod니ction of PEG. This study fcnjnd that PA and the PEP conj니gate are attractive systems for drug delivery because the materials have good biocompatibility with the possibility of manipulating the release characteristics of hydrophobic drugs.

제안 방법

, 1991). To evaluate the CAC of the polymeric nanoparticles of the PEP corrugate, fl니orescence spectroscopy was performed and pyrene was used as a hydrophobic probe. Fig.

이론/모형

Polymeric nanoparti이es of PEP were prepared by the dialysis method (Jeong et al., 1998). 40 mg of PEP conjugate was dis응이ved in 10 mL of DMAc.

성능/효과

The self-assembling characteristics of PEP were confirmed using 1H-NMR spectroscopy and fluorescent spectroscopy. Observations of SEM showed the spherical morphologies of the PEP nanoparticles and parti시e size distribution by PCS was 193.3 ± 13.53 nm with a unimodal distribution. Clonazepam (CNZ), as a model dnjg, was easy to entrap into polymeric nanoparticles of PEP conjugates and the drug release rate was increased by intcod니ction of PEG.
Subsequently, the p니rified sample was filtered and dried in a vacuum oven for 3 days at room temperature. The dried sample was caeef니ly weighted and the yield was 2.21 g for PEP-1 (yield :88.4 % (w/w)), 2.29 g for PEP-2 (yield : 83.3 % (w/w)) and the conjugation yield of CMPEG into the PA backbone was evaluated as approximately 42 % (w/w) of PEP-1 ((0.21/0.5)*100) and 38.7 % (w/w) of PEP-2 ((0.29/0.75)*100).
It was observed that CNZ had sustained released from the nanoparticles for 4 days and the release rate was increased by introduction of PEG. These results indicated, that introduction of PEG into PA induces a more hydrophilic microenvironment into nanoparticles, resulting in the drug being diffused out more easily through hydrophilic aqueous channels by diff니sion mechanisms. In addition, hydrophobic interaction between the drug and the polymer chain may become weaker by the introduction of PEG.

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