Kim, Dong-Seok
(Research Division for Human Life Sciences, Seoul National University)
,
Park, Seo-Hyoung
(Department of Dermatology, Seoul National University)
,
Kwon, Sun-Bang
(Department of Dermatology, Seoul National University)
,
Li, Kap-Sok
(Department of Dermatology, Seoul National University)
,
Youn, Sang-Woong
(Department of Dermatology, Seoul National University)
,
Park, Kyoung-Chan
(Department of Dermatology, Seoul National University)
In this study, the effects of (-)-epigallocatechin-3-gallate (EGCG) and/or hinokitiol (${\beta}-thujaplicin$) on melanogenesis were investigated. Our results showed that both EGCG and hinokitiol significantly inhibited melanin synthesis in a concentration-dependent manner, and that their ...
In this study, the effects of (-)-epigallocatechin-3-gallate (EGCG) and/or hinokitiol (${\beta}-thujaplicin$) on melanogenesis were investigated. Our results showed that both EGCG and hinokitiol significantly inhibited melanin synthesis in a concentration-dependent manner, and that their hypopigmenting effects were stronger than that of kojic acid, which is known to inhibit melanin formation in melanocytes and melanoma cells. Interestingly, EGCG did not show any additive hypopigmenting effect in combination with kojic acid, though EGCG did show a synergistic effect in combination with hinokitiol. Several reports indicate that the activation of extracellular signal-regulated kinase (ERK) induces microphthalmia-associated transcription factor (MITF) degradation. Accordingly, the effects of EGCG and hinokitiol on the ERK signaling pathway were examined. EGCG and hinokitiol induced neither ERK activation nor MITF degradation. On the other hand, both EGCG and hinokitiol reduced the protein levels of MITF and of tyrosinase, the rate limiting melanogenic enzyme, whereas kojic acid had no effect. In addition, hinokitiol strongly downregulated the activity of tyrosinase, whereas EGCG or kojic acid had only a little effect. These results show that both EGCG and hinokitiol reduce MITF production, and suggest that reduced tyrosinase activity by hinokitiol explains their synergistic effect on melanogenesis.
In this study, the effects of (-)-epigallocatechin-3-gallate (EGCG) and/or hinokitiol (${\beta}-thujaplicin$) on melanogenesis were investigated. Our results showed that both EGCG and hinokitiol significantly inhibited melanin synthesis in a concentration-dependent manner, and that their hypopigmenting effects were stronger than that of kojic acid, which is known to inhibit melanin formation in melanocytes and melanoma cells. Interestingly, EGCG did not show any additive hypopigmenting effect in combination with kojic acid, though EGCG did show a synergistic effect in combination with hinokitiol. Several reports indicate that the activation of extracellular signal-regulated kinase (ERK) induces microphthalmia-associated transcription factor (MITF) degradation. Accordingly, the effects of EGCG and hinokitiol on the ERK signaling pathway were examined. EGCG and hinokitiol induced neither ERK activation nor MITF degradation. On the other hand, both EGCG and hinokitiol reduced the protein levels of MITF and of tyrosinase, the rate limiting melanogenic enzyme, whereas kojic acid had no effect. In addition, hinokitiol strongly downregulated the activity of tyrosinase, whereas EGCG or kojic acid had only a little effect. These results show that both EGCG and hinokitiol reduce MITF production, and suggest that reduced tyrosinase activity by hinokitiol explains their synergistic effect on melanogenesis.
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제안 방법
Kojic acid was 니sed as a positive control, since it is known to reduce melanin synthesis. C이Is were exposed to 10 |iM EGCG, 10 |iM hinokitiol, or 100 卩M kojic acid for 4 days and then photo graphed under a phase contrast microscope (Fig. 1). Our results showed that both EGCG- and kojic acid-treated cells were less pigmented than the control, and that the hinokitiol-treated cells were markedly less pigmented.
To investigate the influences of EGCG or hinokitiol, the melanin contents of the Mel-Ab cells were measured after EGCG or hinokitiol treatment. Kojic acid was 니sed as a positive control, since it is known to reduce melanin synthesis.
데이터처리
Differences between results were assessed for signi ficance using the Student's f-test. P values of <0.
이론/모형
Cell viability was determined by using a crystal violet assay (Dooley et al., 1994). After incubating cells with test substances for 24 h, the culture medium was removed and replaced with 0.
성능/효과
1). Our results showed that both EGCG- and kojic acid-treated cells were less pigmented than the control, and that the hinokitiol-treated cells were markedly less pigmented. We also measured the cell니melanin contents after EGCG, hinokitiol, or kojic acid treatment.
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