[논문]Oxidative Stress in Ovariectomy Menopause and Role of Chondroitin Sulfate

Ha, Bae-Jin

doi:10.1007/bf02980181

Oxidative Stress in Ovariectomy Menopause and Role of Chondroitin Sulfate 원문보기

Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, v.27 no.8, 2004년, pp.867 - 872

Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)

Abstract ▼ AI-Helper

Oxidative stress due to reactive oxygen species (ROS) can cause oxidative damage to cells. Cells have a number of defense mechanisms to protect themselves from the toxicity of ROS. Mitochondria are especially important in the oxidative stress as ROS have been found to be constantly generated as an endogen threat. Mitochondrial defense depends mainly on super-oxide dismutase (SOD) and glutathione peroxidase (GPx), whereas microsomal defense depends on catalase (CAT), which is an enzyme abundant in microsomes. SOD removes superoxide anions by converting them to $H_2O$$_2$, which can be rapidly converted to water by CAT and GPx. Also, GPx converts hydroperoxide (ROOH) into oxidized-glutathione (GSSG). Ovariectomized (OVX) rats are used as an oxidative stress model. An ovariectomy increased the levels of MDA, one of the end-products in the lipid peroxidative process, and decreased levels of the antioxidative enzymes； SOD, CAT and GPx. However, Chondroitin sulfate (CS) decreased the levels of MDA, but increased the levels of SOD, CAT and GPx in a dose-depen-dent manner. Moreover, inflammation and cirrhosis of liver tissue in CS- treated rats were sig-nificantly decreased. These results suggest that CS might be a potential candidate as an anti oxidative reagent.

주제어

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제안 방법

Both the ovaries of the animals were ectomized. After 2 days, CS C type (sigma no. 4384) was used, and 100 and 200 ㎎/㎏ were intraperitoneally injected into the OVX+CS100 and OVX+CS200 groups, respectively. Physiological saline was injected into the OVX group.

대상 데이터

Sparague-Dowley (SD) rats (female, 130 g서 50 g) were supplied from the Korean Experimental Animal Center (KEAC), and acclimatized for 7 days. All the animals were maintained in separated cages, with laboratory chow and tap water ad libitum.

데이터처리

Statistical analysis was performed using the Student's & test, with p<0.01 and p<0.05 considered statistically significant. All resuIts are expressed as mean ± S.

이론/모형

, 1971). The CAT levels were measured using the method of Aebi (Aebi, 1984). The GPx levels were estimated by the method of Lawrence and Burk (Lawrence and B나rk, 1976).
The CAT levels were measured using the method of Aebi (Aebi, 1984). The GPx levels were estimated by the method of Lawrence and Burk (Lawrence and B나rk, 1976). In order to make the histopathological examination, the liver tissue was fixed overnight in 10% formaldehyde solution (dissolved in phosphate buffer (pH 7.

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