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Mass Production of Sand Dune Plant, Vitex rotundifolia via Micropropagation 원문보기

Journal of plant biotechnology, v.6 no.3, 2004년, pp.165 - 169  

Park, Hye-Jeong (Department of Biology, Dankoook University) ,  Min, Byeong-Mee (Department of Biology, Dankoook University) ,  Cha, Hyeon-Cheol (Department of Biology, Dankoook University)

Abstract ▼ AI-Helper 아이콘AI-Helper

The fruits of Vitex rotundifolia in Korea, known as 'Man Hyung Ja', occupy an important position as traditional oriental medicine in Asian countries. It is known that propagation of this plant by seed is difficult and time-consuming with little success. Attempts were made to develop a method by usin...

주제어

#Vitex rotundifolia   #regeneration   #sand-dune plant   #Man Hyung Ja   #mass propagation  

AI 본문요약
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제안 방법

  • In contrast, Ashok Kumar et al (2002) found that embryogenic calls formation from explants depend upon interaction between 2, 4-D and BA in Gymmema sylvestre. In this study, it was found that high frequencies from leaf explant culture in response to 2, 4-D treatment. However, it was unable to induce shoot regenera­tion from V.
  • It was presumed that cytokinins would be active on shoot formation from the nodal culture. To investigate the effects of cytokinin on organogenesis, nodal explants were cultured on Nitsch medium supplemented with BA alone, BA with TDZ or BA with auxin (IAA or NAA with low callus induction level). Among them, only BA treatment showed highest frequency, BA+TDZ and BA+IAA showed moderate frequency, and BA+NAA showed lowest frequency in multiple-shoot induction (Figure 2).

대상 데이터

  • Afterward, the cuItures were kept for 4 weeks at 25O±1°C and under a 16 h/8 h day/night photoperiod (Torres 1989). All experiments were conducted twice, with 20 replicates. The percentage of calkis or shoot induction were investigated after 4 weeks.
  • on nodes in the culture. For organogenesis from nodes, MS, Nitsch or Gamberg media with 1 mg/L 2, 4-D, IAA and NAA were tested. Thereafter, the node tissues on a Nitsch medium (Nitsch 1969) supplemented with growth regulators, BA, IAA, NAA, or TDZ (thidiazuron) were cultured.

데이터처리

  • All data means by Duncan's multiple range tests at p<0.05.
  • Percentages were recorded after 4 weeks culture. The data means by Duncan's multiple range test at p<0.05. (First letter: M, Murashige and Skoog; G, Gamberg; N, Nitsch.
본문요약 정보가 도움이 되었나요?

참고문헌 (31)

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