[논문]In Vitro Propagation of Zingiberaceae Species with Medicinal Properties

Keng, Chan Lai; Hing, Thong Weng

In Vitro Propagation of Zingiberaceae Species with Medicinal Properties 원문보기

Journal of plant biotechnology, v.6 no.3, 2004년, pp.181 - 188

Keng, Chan Lai (School of Biological Sciences University Sains Malaysia) , Hing, Thong Weng (School of Biological Sciences University Sains Malaysia)

Abstract ▼ AI-Helper

Zingiber officinale buds from the rhizomes were used to produce in vitro shoots. These explants produced the largest number of multiple shoots, 9.8 shoots per explant, when were cultured on MS (Murashige and Skoog 1962) medium supplemented with 2.0 mg/L benzyladenine (BA) and 2.0 mg/L indole butyric acid (IBA). This medium was also found to be suitable for in vitro propagation of other Zingiberaceae species: Alpinia conchigera, Alpinia galanga, Curcuma domestica, C. zedoaria and Kaempferia galanga. Both C. domestica and C. zedoaria produced more multiple shoots when were cultured in the liquid proliferation medium, MS medium containing 2.0 mg/L BA and 2.0 mg/L IBA. To maintain the in vitro plantlets of Zingiberaceae species, they were required to subculture every four weeks. After executing proper acclimatization protocol, in vitro plantlets of Alpinia galanga, A. conchigera, Curcuma domestica, C. zedoaria, Kaempferia galanga and Zingiber officinale could be successfully planted in the field with high percentage of survival.

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제안 방법

(Mishra and Rath 1988; Bhagyalakshmi and Singh 1988). The objectives of this study was, therefore, to find a common proliferation medium that can induce muItiple shoots formation and a micropropagation protocol that can be applied for most of the Zingiberaceae species.

데이터처리

The number of shoots produced was recorded after 4 weeks. Data obtained were analyzed using two-way ANOVA and the means were compared using Duncan's Multiple Range Test at p=0.05.
The number of shoots formed from the bud explant of each species was recorded after 4 weeks of culture. The data analyzed using one-way ANOVA and the effect of the proliferation medium on the various Zingiberaceae species was determined using Duncan's Multiple Range Test at p=0.05.

성능/효과

7 shoots from each bud. Results indicated that MS medium supplemented with 2.0mg/L BA and 2.0mg/L IBA was the most optimum for multiple shoots formation of Z. officinaleusing bud explants(Table 2). The multiple shoots formed were healthy with normal leaves and roots(Figure 1).
The aseptic buds of Z. officinale, A. conchigera, A. galanga, C. domestica, C, zedoaria and K. galanga were cultured on MS medium supplemented with 2mg/L BA and 2 mg/L IBA, the most optimum medium for multiple shoots formation of Z. officinale. Three bud explants were cultured into 150mL jam jar containing the shoot proliferation medium and 10 units of jam jars wereused for each species.
Thereafter they were transferred to pot containing top soil: sand: organic soil(2:2:1). This acclimatization protocol enabled high percentage(77-90%) of the in vitro plantlets of Z. officinale, C. domestica, C. zedoaria, K. galanga, A. galanga and A. conchigera to survive and were successfully planted in the outside environment(Table 5). Morphology of the in vitro plantlets was identical to their mother plants.

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