Abstract

Zingiber officinale buds from the rhizomes were used to produce in vitro shoots. These explants produced the largest number of multiple shoots, 9.8 shoots per explant, when were cultured on MS (Murashige and Skoog 1962) medium supplemented with 2.0 mg/L benzyladenine (BA) and 2.0 mg/L indole butyric acid (IBA). This medium was also found to be suitable for in vitro propagation of other Zingiberaceae species: Alpinia conchigera, Alpinia galanga, Curcuma domestica, C. zedoaria and Kaempferia galanga. Both C. domestica and C. zedoaria produced more multiple shoots when were cultured in the liquid proliferation medium, MS medium containing 2.0 mg/L BA and 2.0 mg/L IBA. To maintain the in vitro plantlets of Zingiberaceae species, they were required to subculture every four weeks. After executing proper acclimatization protocol, in vitro plantlets of Alpinia galanga, A. conchigera, Curcuma domestica, C. zedoaria, Kaempferia galanga and Zingiber officinale could be successfully planted in the field with high percentage of survival.

주제어

#Acclimatization   #aseptic explants   #BA   #Zingiberaceae species   #IBA   #multiple shoots   #subculturing  

참고문헌 (33)

1. Chen JF (1996) Plant food and nutritional immunology. A scientific approach. Bright Ideal Press, Provo, Utah, USA 
2. Fulder S, Tenne M (1996) Ginger as an anti-nausea remedy in pregnancy - The issue of safety. HerbaIGram 38: 47-50 
3. Halevy IH (1985) CRC Handbook of flowering. Vol IV. CRC Press, Inc., Boca Raton, Florida 
4. Hu CY, Wang PJ (1983) Meristem, shoot tip and bud cultures. In: Evans DA, Wang WR, Ammirato, PV, Yamada Y (eds), Handbook of Plant Cell Culture, Vol I, PP 177-277. Macmillan Publishing Co., New York 
5. Ikeda LR, Tanabe MJ (1989) In vitro subculture applications for ginger. HortScience 24: 142-143 
6. Lim WT (2000) Penghasilan anak benih lengkuas (Alpinia gatanga) secara in vitro. Pusat Pengajian Sains Kajihayat, USM 
7. Muhamad Z, Mustafa AM (1994) Traditional Malay medicinal plants. Fajar Bakti Sdn Bhd, KL 
8. Porter CL (1967) Taxonomy of flowering plants, $2^{nd}$ edn. W.H. Freeman and Company, San Francisco 
9. Rout GR, Das P (1997) In vitro organogenesis in ginger (Zingiber officinale Rosc.). J Herbs Spices Med Plants 4: 41-51 
10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 
11. Yu PQ, Zhui WF (1995) Shiyong zhongxiyi zhongliu zhiliao daquan. University Chong Qing, Chong Qing, China 
12. Itokawa H, Morita H, Sumitomo T, Totsaka N, Takeya R (1987) Antitumor principles from Alpinia galanga. Planta Medica 53: 32-33 
13. Bhagyalakshmi, Singh NS (1988) Meristem culture and micro-propagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of oleoresin. J Horticultural Science 63: 321-327 
14. Teo CKH, Ch'ng BI (2000) Food and cancer. Cancer Care, Penang 
15. Anon (1975) Quanguo zhongcaoyao huibian. Vol 1. Renmin Weisheng Publisher, Beijing 
16. Noguchi Y, Yamakawa O (1988) Rapid donal propagation of ginger (Zingiber officinale Roscoe) by roller tube culture. Japanese J Breed 38: 437-442 
17. Pan MQ, Li YH, Chen L (1996) Zhonghua zhongliu zhiliao dacheng. Hebei Science & Technology Publisher, Hebei 
18. Liu CA, Peng M (1994) Kangai zhongcaoyao dacidian. Hubei Science & Technology Publisher, Hubei 
19. Ridley HN (1967) The Flora of the Malay Peninsula. Vol. IV-Monocotyledones. (Rephnt). L. Reeve & Co., Ltd., London 
20. Chan CW, Chan LK (2002) Control of bacterial and fungal contamination in Cyperus aromaticus in vitro culture. J Bioscience 13: 1-7 
21. Hsuan K, Chin SC, Tan HTW (1998) The concise flora of Singapore. Vol II : Monocotyledons. Singapore University Press. Singapore 
22. Nallammai S (1997) Micropropagation and production of artifial seeds of Anubia sp., MSc. Thesis, Universiti Sains Malaysia, Penang, Malaysia 
23. Faujan HA, Ahmad AR (1988) Tumbuhan kunyit dan statusnya masa kini. Pusat Pengembangan dan Pendidikan Lanjutan, Universiti Pertanian Malaysia, Serdang, Selangor 
24. Anand PHM, Hariharan M (1997) tn vitro multiplication of greater galangal (Alpinia gatanga (Linn.) Willd) - A medicinal plant. Phytomorphology 47: 45-50 
25. Mackeen MM, Ali AM, EI-Sharkawy SH, Manap MY, Salleh KM, Lajis NH, Kawazu K (1977) Antimicrobial and cytotoxin properties of some Malaysian tradisional vegetabIes (ulam). Int J Pharm. 35: 174-178 
26. Bateman G, Dod B (1978) Flowering plants of the world. Oxford University Press. Oxford, London, Melbourne 
27. Wang B (1994) Kangai zhongyaofang xuan. Renmin Juyi Publisher, China 
28. Anon (1978) Zhongyao dacidian. Shanghai Science Technology Publisher, China 
29. Rahmad SB (1999) In vitro propagation of gerbera (Gerbera jamesonii). MSc Thesis, Universiti Sains Malaysia, Penang, Malaysia 
30. Mowrey DB, Clayson, DE (1982) Motion sickness, ginger and psychophysics. Lancet 1: 655-657 
31. Sharma TR, Singh BM (1997) High frequency in vitro multi-plication of disease free Zingiber officinale Rosc. Plant Cell Rep 17: 68-72 
32. Chang BKW, Criley RA (1993) Clonal propagation of pink ginger in vitro. HortScience 28: 1203 
33. Mishra B, Rath GC (1988) Geotrichum rot of stored ginger. Indian J Mycol Plant Pathol 18: 21-23 

이 논문을 인용한 문헌 (0)

1. 이 논문을 인용한 문헌 없음