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Direct Somatic Embryogenesis of Curculigo orchioides Gaertn., an Endangered Medicinal Herb 원문보기

Journal of plant biotechnology, v.6 no.3, 2004년, pp.193 - 197  

Thomas, T.Dennis (Post Graduate and Research Department of Botany) ,  Jacob, Alphonsa (Post Graduate and Research Department of Botany)

Abstract ▼ AI-Helper 아이콘AI-Helper

In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 $\mu{M}$) or Kin (2-10 $\mu{M}$

주제어

#Endangered species   #leaf segment culture   #medicinal plant   #direct somatic embryogenesis  

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

  • Somatic embryogenesis and synthetic seeds could be employed for the large scale in vitro micropagation of this plant. Thus, the present study was carried out with the objective to induce somatic embryogenesis in C. orchioides from immature leaf segments.

가설 설정

  • A. A group of germinating embryos at different stages of development on MS medium supplemented BAP (8 μM) 90 days after culture.
  • A. Initiation of globular embryos from the explant on MS medium supplemented with 8 μM BAP 45 days after culture.
본문요약 정보가 도움이 되었나요?

참고문헌 (23)

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  9. Gupta R, Chadha KL (1995) Medicinal and aromatic plants research in India, In: Chadha KL, Gupta R (eds), Advances in, Horticulture, Medicinal and Aromatic Plants, vol. 11, pp. 1-43. Malhotra House, New Delhi, India 

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  16. Prajapati HA, Patel DH, Mehta SR, Subrarnanian RB (2003) Direct in vitro regeneration of Curcutigo orchioides Gaertn., an endangered anticarcinogenic herb. Curr Sci 80: 747-749 

  17. Samoylov VM, Tucker DM, Parrott WA (1998) Soybean (Glycine max (L.) Merrill.) embryogenic cultures: the role of sucrose and total nitrogen content on proliferation, In Vitro Cell Dev Biol Plant 34: 8-13 

  18. Sarasan V, Ramsay MM, Roberts AV (2002) In vitro germination and induction of direct somatic embryogenesis in 'Bottle Palm' [Hyophorbe lagenicaulis (L. Bailey) H.E. Moore], a critically endangered Mauritian palm. Plant Cell Rep 20: 1107-1111 

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  20. Suri SS, Jain S, Ramawat KG (1999) Plantlet regeneration and bulbil formation in vitro from leaf and stem explants of Curculigo orchioides, an endangered medicinal plant. Sci Hort 79: 127-134 

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