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논문 상세정보

Direct Somatic Embryogenesis of Curculigo orchioides Gaertn., an Endangered Medicinal Herb

Journal of plant biotechnology v.6 no.3 , 2004년, pp.193 - 197  
Abstract

In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 $\mu{M}$) or Kin (2-10 $\mu{M}$). Optimum response in terms of per cent cultures responding (89%) and the number of embryos per explant (16) were observed on MS medium supplemented with 8 $\mu$M BAP. The emergence of several somatic embryos on the adaxial side of the leaf segments was observed one month after the culture. Germinated somatic embryos were grown up to about 1.5 cm length before transferring to maturation medium. For maturation, the individual embryos were isolated and transferred to MS medium supplemented with BAP (5 $\mu{M}$) and NAA (0.5 $\mu{M}$). The plantlets emerged from the embryos were transferred to soil containing 1 peat: 1 sand with 90% success. The embryos were formed directly on the leaf segments without any callus phase. Direct regeneration of somatic embryos is important for the conservation of this endangered species, as rare somaclonal variants are likely to arise than from indirect regeneration.

참고문헌 (23)

  1. Bhojwani SS, Razdan MK (1996) Plant Tissue Culture: Theory and Practice, A Revised Edition. Elsevier Sdence, Amsterdam 
  2. Gray DJ (1989) Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos, In Vitro Cell Dev Biol 25: 1173-1178 
  3. Samoylov VM, Tucker DM, Parrott WA (1998) Soybean (Glycine max (L.) Merrill.) embryogenic cultures: the role of sucrose and total nitrogen content on proliferation, In Vitro Cell Dev Biol Plant 34: 8-13 
  4. Sivakumar G, Krishnamurthy KV, Rajendran TD (2003) Embryoi-dogenesis and plant regeneration from leaf tissue of Glohosa superba. Planta Med 69: 479-481 
  5. Tandon M, Shukla YN (1995) Phytoconstituents of Asparagus adscendens, Chtorophytum arundinaceum and Curculigo orchioides: A review, Current Res Med Aromatic Plants 17: 42-50 
  6. Xu JP, Xu RS (1992) Cycloartane type sapogenins and their glycoside from Curculigo orchioides. Phytochem 31: 2455-2458 
  7. Gray DJ, Purohit A (1991b) Quiescence and dormancy in somatic embryos, In: Bajaj YPS (ed), Biotechnology in Agri-culture and Forestry, vol. 17. High-tech and micropropagation, pp 382-394. Springer-VerIag, New York 
  8. Gupta R, Chadha KL (1995) Medicinal and aromatic plants research in India, In: Chadha KL, Gupta R (eds), Advances in, Horticulture, Medicinal and Aromatic Plants, vol. 11, pp. 1-43. Malhotra House, New Delhi, India 
  9. Garg SN, Misra LN, Reddy MN (1989) Corchicoside A, an orcinol glycoside from Curculigo orchioides. Phytochem 28: 1771-1772 
  10. Nakagawa H, Saijyo T, Yamauchi N, Shigyo M, Kako S, Ito A (2001) Effects of sugars and abscissic acid on somatic embryogenesis from melon (Cucumis melo L.) expanded cotyledon. Sci Hort 90: 85-92 
  11. Sarasan V, Ramsay MM, Roberts AV (2002) In vitro germination and induction of direct somatic embryogenesis in 'Bottle Palm' [Hyophorbe lagenicaulis (L. Bailey) H.E. Moore], a critically endangered Mauritian palm. Plant Cell Rep 20: 1107-1111 
  12. Dhar ML, Dhar MM, Dhawan BN, Mehrotra DN, Ray C (1968) Screening of Indian plants for biological activity. Part I. J Exp Biol 6: 232-249 
  13. Anthony JM, Senaratna T, Dixon KW, Sivasithamparam K(2004) Somatic embryogenesis for mass propagation of Ericaceae- a case study with Leucopogon verticillatus. Plant Cell Tissue Organ Cult 76: 137-146 
  14. Gogate SS, Nadgauda RS (2003) Direct induction of somatic embryogenesis from immature zygotic embryo of cashewnut (Anacardium occidentale L). Sci Hort 97: 75-82 
  15. Kochba J, Spiegel-Roy P, Saad S, Neumann H (1978) Sti-mulation of embryogenesisin Citrus tissue culture by galactose. Naturwissenschaften 65: 261-262 
  16. Gray J, Purohit A (1991 a) Somatic embryogenesis and develop-ment of synthetic seed technology. Crit Rev Plant Sci 10: 33-61 
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497 
  18. Merkle SA (1999) Application of in vitro culture (IVC) for conservation of forest trees, In: Bowes BG (ed), Plant Propagation and Conservation, pp. 34-45. Manson, London 
  19. Suri SS, Jain S, Ramawat KG (1999) Plantlet regeneration and bulbil formation in vitro from leaf and stem explants of Curculigo orchioides, an endangered medicinal plant. Sci Hort 79: 127-134 
  20. Prajapati HA, Patel DH, Mehta SR, Subrarnanian RB (2003) Direct in vitro regeneration of Curcutigo orchioides Gaertn., an endangered anticarcinogenic herb. Curr Sci 80: 747-749 
  21. Xu JP, Xu RS, Li XY (1992) Four new cycloartane saponins from Curculigo orchioides. Planta Med 58: 208-210 
  22. Jehan H, Courtois D, Ehret C, Lerch K, Petiard V (1994) Plant regeneration of lris pallida Lam. and lris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Rep 13: 671-675 
  23. Mathur G, von Arnold S, Nadgauda R (2000) Studies on somatic embryogenesis from immature zygotic embryos of chir pine (Pinus roxburghii Sarg.) Curr Sci 79: 999-1004 

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