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제안 방법
- site. For this purpose, we prepared pegylated liposomes carrying plasmid DNA, and then investigated the effect of the following various parameters on the DNA amount encapsulated into the liposomes: cationic lipid concentration, initial DNA amount, total lipid amount, DNA size and liposome size. Furthermore, to prepare immunoliposomes capable of both gene delivery and targeting, we conjugated rat IgG to the liposomes carrying plasmid DNA and examined the effect of the ratio of DSPE-PEG 2000-maleimide to rat IgG on the number of IgG molecules conjugated per liposome.
- For this purpose, we prepared pegylated liposomes carrying plasmid DNA, and then investigated the effect of the following various parameters on the DNA amount encapsulated into the liposomes: cationic lipid concentration, initial DNA amount, total lipid amount, DNA size and liposome size. Furthermore, to prepare immunoliposomes capable of both gene delivery and targeting, we conjugated rat IgG to the liposomes carrying plasmid DNA and examined the effect of the ratio of DSPE-PEG 2000-maleimide to rat IgG on the number of IgG molecules conjugated per liposome.
- In this study, we prepared and characterized immunoliposomes which can be more stable in the blood stream by polyethyleneglycol (PEG)-derivatized lipids and target the effect site by conjugating IgG (monoclonal antibody) to the tips of the PEG strands. In Sepharose CL-4B column chromatography, the co-location of the two peaks (Fig.
- To determine if the number of IgG molecules conjugated per liposome was affected by the ratio of DSPE-PEG 2000-maleimide to IgG, a series of studies were performed with variations in the ratio of 5 to 1, 10 to 1 and 20 to 1 (Fig. 5). For the ratios of 10 to 1 and 20 to 1, 0.
대상 데이터
- concentration. DDAB content used in this study was 1, 3, 4, 5, and 7 mole% (0.2, 0.6, 0.8, 1.0, and 1.4 μmole). To maintain the total lipid amount as 20 μmole, neutral lipid (POPC) concentration was decreased according to increasing DDAB concentration.
- I). Lipid composition was kept at 92 mole% POPC, 4 mole% DDAB, 3 mole% DSPE-PEG 2000 and 1 mole% DSPE-PEG 2000-maleimide, and 100 μg of plasmid DNA (pGl_2 clone 753) was used. To control liposome size, the liposomes carrying plasmid DNA were forced through 2 stacked polycarbonate filters with pore size of 400 nm, 200 nm or 100 nm.
- Pardridge (University of California, Los Angeles, USA). Rat IgG was purchased from Sigma-Aldrich (St. Louis, MO, USA).
- Effect of initial DNA and total lipid amount on DNA amount encapsulated into the liposomes. The liposomes were composed of 92 mole% POPC, 4 mole% DDAB, 3 mole% DSPE-PEG 2000 and 1 mole% DSPE-PEG 2000-maleimide. (A) Effect of the initial DNA amount on the DNA amount encapsulated into the liposomes.
- 4). The plasmids used in this study were pGL2 clone 753 (5.8 kb), pSV clone 756 (6.8 kb) and pCEP4 clone 790 (10.6 kb). The DNA amount encapsulated into the liposomes was reduced with increasing plasmid DNA size.
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