세 수준의 자당이 첨가된 배지에서 유기물의 첨가 유무에 따른 Solanum tuberosum L.의 기내 광혼합영양생장 Photomixotrophic Growth of Solanum tuberosum L. in vitro with Addition and Omission of Organic Materials at Thee Initial Sucrose Levels in the Medium원문보기
식물의 조직배양에서 가장 널리 이용되는 MS 배지 등의 무기성분은 종속영양에 적합하도록 조성되었다. 그러므로 이들 배지가 혼합영양이나 독립영양 배양에는 부적합 할 수 도 있다. 광혼합영양 미세증식에서 배지에 첨가되는 당의 수준은 낮추고 배양기의 광과 $CO_2$ 수준은 올리며, 광합성의 의한 탄소의 획득을 증진시키기 위해 열록소가 있는 절편체를 이용한다. Factorial 실험에서 유기물 (OM, $m^{-3}$ 배지당 각각 0.5g의 thiamine, nicotinic acid 및 pyridoxine과 100g ${\cdot}$$m^{-3}$ myo-inositiol)의 첨가(+)와 생략(_) 및 세 가지 수준의 당(0, 15 및 30 kg ${\cdot}$$m^{-3}$)이 감자 소식물체의 생장에 미치는 영향을 조사하였다. 단엽단절 절편체를 0.1${\times}$$10^{-4}m^{-3}$의 MS 아가(8 kg ${\cdot}$$m^{-3}$) 배지(증기소독 전 pH 5.80)배지가 담긴 유리 시험관(100mm${\times}$25mm)에 치상하여 직경 6mm의 가스투과 필터(5.1 air exchanges ${\cdot}$$h^{-1}$)가 부착된 투명한 필름으로 봉하여 배양하였다. 배양체는 27일간 $23^{\circ}C$, 50% RH에서 16 h ${\cdot}$$d^{-1}$의 명기 동안에 cool white 형광등으로 130${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD의 광과 350-450${\mu}mol\;{\codt}\;mol^{-1}의CO_2$가 공급되는 배양실에서 배양되었다. 유기물이 공급된 처리(+OM)와 공급되지 않은 처리(-OM)에서 감자 소식물체의 생육은 유사하였으나 배지의 pH는 후자에서 0.2 더 낮았다. 배지에 첨가된 당의 수준이 증가할수록 건물중, % 건물율 및 경경은 증진된데 반해 신초와 뿌리의 건물중 비율, 엽면적, 건물중당 엽록소 함량 및 배지의 pH는 감소하였다. 유기물과 당 수준간의 상호작용도 신초길이와 배지 pH에서 관찰되었다. 이상의 결과로부터 광도와 $CO_2$농도를 높인 광혼압영양배양에서 감자 소식물체의 생장에는 배지에 첨가되는 당은 유익하지만 그 이외의 유기물은 첨가하지 않아도 악영향이 없다는 것은 나타낸다.
식물의 조직배양에서 가장 널리 이용되는 MS 배지 등의 무기성분은 종속영양에 적합하도록 조성되었다. 그러므로 이들 배지가 혼합영양이나 독립영양 배양에는 부적합 할 수 도 있다. 광혼합영양 미세증식에서 배지에 첨가되는 당의 수준은 낮추고 배양기의 광과 $CO_2$ 수준은 올리며, 광합성의 의한 탄소의 획득을 증진시키기 위해 열록소가 있는 절편체를 이용한다. Factorial 실험에서 유기물 (OM, $m^{-3}$ 배지당 각각 0.5g의 thiamine, nicotinic acid 및 pyridoxine과 100g ${\cdot}$$m^{-3}$ myo-inositiol)의 첨가(+)와 생략(_) 및 세 가지 수준의 당(0, 15 및 30 kg ${\cdot}$$m^{-3}$)이 감자 소식물체의 생장에 미치는 영향을 조사하였다. 단엽단절 절편체를 0.1${\times}$$10^{-4}m^{-3}$의 MS 아가(8 kg ${\cdot}$$m^{-3}$) 배지(증기소독 전 pH 5.80)배지가 담긴 유리 시험관(100mm${\times}$25mm)에 치상하여 직경 6mm의 가스투과 필터(5.1 air exchanges ${\cdot}$$h^{-1}$)가 부착된 투명한 필름으로 봉하여 배양하였다. 배양체는 27일간 $23^{\circ}C$, 50% RH에서 16 h ${\cdot}$$d^{-1}$의 명기 동안에 cool white 형광등으로 130${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD의 광과 350-450${\mu}mol\;{\codt}\;mol^{-1}의CO_2$가 공급되는 배양실에서 배양되었다. 유기물이 공급된 처리(+OM)와 공급되지 않은 처리(-OM)에서 감자 소식물체의 생육은 유사하였으나 배지의 pH는 후자에서 0.2 더 낮았다. 배지에 첨가된 당의 수준이 증가할수록 건물중, % 건물율 및 경경은 증진된데 반해 신초와 뿌리의 건물중 비율, 엽면적, 건물중당 엽록소 함량 및 배지의 pH는 감소하였다. 유기물과 당 수준간의 상호작용도 신초길이와 배지 pH에서 관찰되었다. 이상의 결과로부터 광도와 $CO_2$농도를 높인 광혼압영양배양에서 감자 소식물체의 생장에는 배지에 첨가되는 당은 유익하지만 그 이외의 유기물은 첨가하지 않아도 악영향이 없다는 것은 나타낸다.
The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lo...
The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$$m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$$m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$$m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$$m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$$d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.
The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$$m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$$m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$$m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$$m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$$d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.
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제안 방법
Fresh weight, dry weight, the number of leaves, leaf area, shoot length, stem diameter and medium pH were measuied on 28th day after the start of the experiment. Percent dry matter and shoot to root dry weight ratio were calculated based on dry and fresh weights, and shoot and root dry weigits, lespectively.
carboi acquisitioi. The necessity for the photomixotrophic culture of the chlorophyllous tissues of other organic materials that are usuale1 added to the medium is yet to be examined, hi the piesent study, potato explants weie cultured in the treatments with several combinations of addition and emission of thiamine, nicotinic acid, pyridoxine and myo-inositol at 3 initial sucrose levels to investigate their effects on the photo- mixotrophic growth of potato plantlets.
대상 데이터
9 klx) on the empty culture shelf in the culture loom. Micro-porous polypropylene film (brand name : IPX film, Mitsui Petrochemical Co.) was used to cap the test tubes. A hole (6 mm in diameter) was made at the center of square IPX film (the length of the side: 7 cm).
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