Effects of Dietary Perilla Oil and Enteromorpha compressa Meal on Growth, Fatty Acid Composition and Hematology of the Cultured Sweet Smelt (Plecoglossus altivelis)원문보기
Sweet smelt (Plecoglossus altivelis) were fed four different diets supplemented with either perilla oil $(2.0\%)$ rich in 18:3n-3 (CP), and perilla oil and Enteromorpha compressa meal $(2.0\%)$ (CPA), soybean oil rich in 18:2n-6 (CO), or soybean oil and algal meal (CA) for 4 we...
Sweet smelt (Plecoglossus altivelis) were fed four different diets supplemented with either perilla oil $(2.0\%)$ rich in 18:3n-3 (CP), and perilla oil and Enteromorpha compressa meal $(2.0\%)$ (CPA), soybean oil rich in 18:2n-6 (CO), or soybean oil and algal meal (CA) for 4 weeks. The growth performance, fatty acid composition of muscle, plasma lipid peroxidation and blood components of the sweet smelt were then determined. The specific growth rate and feed efficiency in the fish fed the CPA diet were the highest, while the other groups showed similar results. The fatty acid composition of muscle in sweet smelt reflected the dietary lipids; 18:3n-3 was higher in the fish fed the CP and CPA diets, and 18:2n-6 was higher in the fish fed the CO and CA diets. The other fatty acid profiles presented almost no differences with respect to the diet composition. The fish fed the CA, CP and CPA diets contained significantly lower levels of triglyceride, thiobarbituric acid-reactive substances and hydroxyl radical in their plasma than that fed the CO diet. Phagocytic activity was the highest in the fish fed the CPA diet and higher in those of the fish fed the CP and CA diets compared to the CO diet group. The results from this study suggest that a dietary supplement of $2.0\%$ perilla oil together with $2.0\%$ E. compressa meal may improve the growth and health of cultured sweet smelt.
Sweet smelt (Plecoglossus altivelis) were fed four different diets supplemented with either perilla oil $(2.0\%)$ rich in 18:3n-3 (CP), and perilla oil and Enteromorpha compressa meal $(2.0\%)$ (CPA), soybean oil rich in 18:2n-6 (CO), or soybean oil and algal meal (CA) for 4 weeks. The growth performance, fatty acid composition of muscle, plasma lipid peroxidation and blood components of the sweet smelt were then determined. The specific growth rate and feed efficiency in the fish fed the CPA diet were the highest, while the other groups showed similar results. The fatty acid composition of muscle in sweet smelt reflected the dietary lipids; 18:3n-3 was higher in the fish fed the CP and CPA diets, and 18:2n-6 was higher in the fish fed the CO and CA diets. The other fatty acid profiles presented almost no differences with respect to the diet composition. The fish fed the CA, CP and CPA diets contained significantly lower levels of triglyceride, thiobarbituric acid-reactive substances and hydroxyl radical in their plasma than that fed the CO diet. Phagocytic activity was the highest in the fish fed the CPA diet and higher in those of the fish fed the CP and CA diets compared to the CO diet group. The results from this study suggest that a dietary supplement of $2.0\%$ perilla oil together with $2.0\%$ E. compressa meal may improve the growth and health of cultured sweet smelt.
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문제 정의
The aim of this study was to ensure that the fatty acid composition (18:3n-3) of the muscle from cultured fish approximates to that of wild fish. In addition, the aim was also to keep the fish healthy through modification of dietary ingredients during the test period. Therefore, in the present study, we investigated the effects of perilla oil which is rich in 18:3n-3 and an algal E.
The aim of this study was to ensure that the fatty acid composition (18:3n-3) of the muscle from cultured fish approximates to that of wild fish. In addition, the aim was also to keep the fish healthy through modification of dietary ingredients during the test period.
제안 방법
Materials were eluted with a mobile phase of hexaneisopropanol (25:1, v/v). All HPLC procedures were carried out at ambient temperature and monitored for absorbance at 295 nm using a photodiode array UV detector (SPD-MlOAvp, Shimadzu Seisakusho, Co. Ltd., Japan).
대상 데이터
After 4 weeks feeding trial, body length and body weight weie measured, and then the fish were transported to a laboratory in dry ice. Muscle was removed from 20 male and 20 female specimens from each tank and then stored at -80 "C prior to analysis. The fish muscle was blended with a speed cutter (National, MK-K51, Japan) prior to assay.
Methyl Iricosanoate (99%, Aldrich Chem. Co., USA) was used as an internal standard.
Juvenile sweet smelts were purchased from a private fish farm and reared on a commercial diet prior to experiment at Kyeongsangnam-Do Hatchery, Korea. The fish weighing about 39.8 g were randomly divided into 8 rectangular concrete tanks (5,000 L), each holding 200 fish. They were cultured on the experimental diets for 4 weeks and each diet had 2 populations assigned to it.
데이터처리
12) statistical package. Significance differences between dietary groups (P<0.05) were determined by analysis of variance (ANOVA), followed, when pertinent, by Turkey's m니tiple comparison test.
이론/모형
An aliquot of the blood was ased to determine the hematocrit and the remainder was centrifuged at 1000xg for 10 min to obtain plasma which was then stored at -80 ℃ until analysis. Plasma component levels were determined with an automatic biochemical analyzer (CL-7100, Shimadzu Co. Ltd., Japan) using the Biuret method (total protein) and the enzymatic method (urea nitrogen, g ucose, total cholesterol and triglyceride).
The SOD activity was assayed using a method based on the formation of nitrite, as described by Oyanagui (1984) and expressed as NU/mg protein. Protein content of plasma was measured using the method of Lowry et al. (1951).
성능/효과
The results presented in this study suggest that diets supplemented with algal meal or perilla oil, particularly with both perilla oil and algal meal, improved the quality of the cultured sweet smelt. It is reeded to investigate the optimum quantity of perilla oil and algal meal in the cultured fish diets.
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